Job ID = 11192777 sra ファイルのダウンロード中... Completed: 27013K bytes transferred in 3 seconds (67692K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 887728 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1924894/SRR3824925.sra Written 887728 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1924894/SRR3824925.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:31 887728 reads; of these: 887728 (100.00%) were paired; of these: 86351 (9.73%) aligned concordantly 0 times 767553 (86.46%) aligned concordantly exactly 1 time 33824 (3.81%) aligned concordantly >1 times ---- 86351 pairs aligned concordantly 0 times; of these: 25159 (29.14%) aligned discordantly 1 time ---- 61192 pairs aligned 0 times concordantly or discordantly; of these: 122384 mates make up the pairs; of these: 87777 (71.72%) aligned 0 times 30509 (24.93%) aligned exactly 1 time 4098 (3.35%) aligned >1 times 95.06% overall alignment rate Time searching: 00:00:31 Overall time: 00:00:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 215746 / 819834 = 0.2632 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 09:54:31: # Command line: callpeak -t SRX1924894.bam -f BAM -g 12100000 -n SRX1924894.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1924894.10 # format = BAM # ChIP-seq file = ['SRX1924894.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 09:54:31: #1 read tag files... INFO @ Sat, 15 Sep 2018 09:54:31: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 09:54:31: # Command line: callpeak -t SRX1924894.bam -f BAM -g 12100000 -n SRX1924894.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1924894.20 # format = BAM # ChIP-seq file = ['SRX1924894.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 09:54:31: #1 read tag files... INFO @ Sat, 15 Sep 2018 09:54:31: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 09:54:31: # Command line: callpeak -t SRX1924894.bam -f BAM -g 12100000 -n SRX1924894.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1924894.05 # format = BAM # ChIP-seq file = ['SRX1924894.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 09:54:31: #1 read tag files... INFO @ Sat, 15 Sep 2018 09:54:31: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 09:54:36: 1000000 INFO @ Sat, 15 Sep 2018 09:54:36: 1000000 INFO @ Sat, 15 Sep 2018 09:54:36: 1000000 INFO @ Sat, 15 Sep 2018 09:54:37: #1 tag size is determined as 38 bps INFO @ Sat, 15 Sep 2018 09:54:37: #1 tag size = 38 INFO @ Sat, 15 Sep 2018 09:54:37: #1 total tags in treatment: 588534 INFO @ Sat, 15 Sep 2018 09:54:37: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 09:54:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 09:54:37: #1 tags after filtering in treatment: 522033 INFO @ Sat, 15 Sep 2018 09:54:37: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 15 Sep 2018 09:54:37: #1 finished! INFO @ Sat, 15 Sep 2018 09:54:37: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 09:54:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 09:54:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 09:54:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 09:54:37: Process for pairing-model is terminated! cat: SRX1924894.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1924894.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1924894.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1924894.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 09:54:38: #1 tag size is determined as 38 bps INFO @ Sat, 15 Sep 2018 09:54:38: #1 tag size = 38 INFO @ Sat, 15 Sep 2018 09:54:38: #1 total tags in treatment: 588534 INFO @ Sat, 15 Sep 2018 09:54:38: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 09:54:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 09:54:38: #1 tags after filtering in treatment: 522033 INFO @ Sat, 15 Sep 2018 09:54:38: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 15 Sep 2018 09:54:38: #1 finished! INFO @ Sat, 15 Sep 2018 09:54:38: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 09:54:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 09:54:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 09:54:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 09:54:38: Process for pairing-model is terminated! INFO @ Sat, 15 Sep 2018 09:54:38: #1 tag size is determined as 38 bps INFO @ Sat, 15 Sep 2018 09:54:38: #1 tag size = 38 INFO @ Sat, 15 Sep 2018 09:54:38: #1 total tags in treatment: 588534 INFO @ Sat, 15 Sep 2018 09:54:38: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 09:54:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cat: SRX1924894.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1924894.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1924894.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1924894.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 09:54:38: #1 tags after filtering in treatment: 522033 INFO @ Sat, 15 Sep 2018 09:54:38: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 15 Sep 2018 09:54:38: #1 finished! INFO @ Sat, 15 Sep 2018 09:54:38: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 09:54:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 09:54:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 09:54:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 09:54:38: Process for pairing-model is terminated! cat: SRX1924894.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1924894.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1924894.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1924894.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。