Job ID = 11192747 sra ファイルのダウンロード中... Completed: 5853K bytes transferred in 2 seconds (17935K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 184655 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1924862/SRR3824893.sra Written 184655 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1924862/SRR3824893.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:07 184655 reads; of these: 184655 (100.00%) were paired; of these: 64470 (34.91%) aligned concordantly 0 times 113575 (61.51%) aligned concordantly exactly 1 time 6610 (3.58%) aligned concordantly >1 times ---- 64470 pairs aligned concordantly 0 times; of these: 10816 (16.78%) aligned discordantly 1 time ---- 53654 pairs aligned 0 times concordantly or discordantly; of these: 107308 mates make up the pairs; of these: 57386 (53.48%) aligned 0 times 45651 (42.54%) aligned exactly 1 time 4271 (3.98%) aligned >1 times 84.46% overall alignment rate Time searching: 00:00:07 Overall time: 00:00:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 31376 / 124946 = 0.2511 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 09:51:02: # Command line: callpeak -t SRX1924862.bam -f BAM -g 12100000 -n SRX1924862.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1924862.10 # format = BAM # ChIP-seq file = ['SRX1924862.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 09:51:02: #1 read tag files... INFO @ Sat, 15 Sep 2018 09:51:02: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 09:51:02: # Command line: callpeak -t SRX1924862.bam -f BAM -g 12100000 -n SRX1924862.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1924862.05 # format = BAM # ChIP-seq file = ['SRX1924862.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 09:51:02: #1 read tag files... INFO @ Sat, 15 Sep 2018 09:51:02: # Command line: callpeak -t SRX1924862.bam -f BAM -g 12100000 -n SRX1924862.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1924862.20 # format = BAM # ChIP-seq file = ['SRX1924862.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 09:51:02: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 09:51:02: #1 read tag files... INFO @ Sat, 15 Sep 2018 09:51:02: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 09:51:03: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 09:51:03: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 09:51:03: #1 total tags in treatment: 89570 INFO @ Sat, 15 Sep 2018 09:51:03: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 09:51:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 09:51:03: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 09:51:03: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 09:51:03: #1 total tags in treatment: 89570 INFO @ Sat, 15 Sep 2018 09:51:03: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 09:51:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 09:51:03: #1 tags after filtering in treatment: 88275 INFO @ Sat, 15 Sep 2018 09:51:03: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 15 Sep 2018 09:51:03: #1 finished! INFO @ Sat, 15 Sep 2018 09:51:03: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 09:51:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 09:51:03: #1 tags after filtering in treatment: 88275 INFO @ Sat, 15 Sep 2018 09:51:03: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 15 Sep 2018 09:51:03: #1 finished! INFO @ Sat, 15 Sep 2018 09:51:03: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 09:51:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 09:51:03: #2 number of paired peaks: 9 WARNING @ Sat, 15 Sep 2018 09:51:03: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 09:51:03: Process for pairing-model is terminated! INFO @ Sat, 15 Sep 2018 09:51:03: #2 number of paired peaks: 9 WARNING @ Sat, 15 Sep 2018 09:51:03: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 09:51:03: Process for pairing-model is terminated! cat: SRX1924862.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX1924862.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Sat, 15 Sep 2018 09:51:03: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 09:51:03: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 09:51:03: #1 total tags in treatment: 89570 INFO @ Sat, 15 Sep 2018 09:51:03: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 09:51:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1924862.20_model.r'rm: cannot remove `SRX1924862.05_model.r'INFO @ Sat, 15 Sep 2018 09:51:03: #1 tags after filtering in treatment: 88275 : そのようなファイルやディレクトリはありません INFO @ Sat, 15 Sep 2018 09:51:03: #1 Redundant rate of treatment: 0.01 : そのようなファイルやディレクトリはありませんINFO @ Sat, 15 Sep 2018 09:51:03: #1 finished! INFO @ Sat, 15 Sep 2018 09:51:03: #2 Build Peak Model... rm: cannot remove `SRX1924862.20_*.xls': そのようなファイルやディレクトリはありませんINFO @ Sat, 15 Sep 2018 09:51:03: #2 looking for paired plus/minus strand peaks... rm: cannot remove `SRX1924862.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1924862.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1924862.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 09:51:03: #2 number of paired peaks: 9 WARNING @ Sat, 15 Sep 2018 09:51:03: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 09:51:03: Process for pairing-model is terminated! cat: SRX1924862.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1924862.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1924862.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1924862.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。