Job ID = 14522016 SRX = SRX1829040 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13761714 spots for SRR3644963/SRR3644963.sra Written 13761714 spots for SRR3644963/SRR3644963.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:17 13761714 reads; of these: 13761714 (100.00%) were unpaired; of these: 1426467 (10.37%) aligned 0 times 10054948 (73.06%) aligned exactly 1 time 2280299 (16.57%) aligned >1 times 89.63% overall alignment rate Time searching: 00:01:17 Overall time: 00:01:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 4254945 / 12335247 = 0.3449 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:02:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:02:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:02:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:02:51: 1000000 INFO @ Sat, 15 Jan 2022 22:02:56: 2000000 INFO @ Sat, 15 Jan 2022 22:03:01: 3000000 INFO @ Sat, 15 Jan 2022 22:03:06: 4000000 INFO @ Sat, 15 Jan 2022 22:03:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:03:16: 6000000 INFO @ Sat, 15 Jan 2022 22:03:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:03:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:03:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:03:21: 7000000 INFO @ Sat, 15 Jan 2022 22:03:22: 1000000 INFO @ Sat, 15 Jan 2022 22:03:26: 8000000 INFO @ Sat, 15 Jan 2022 22:03:27: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:03:27: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:03:27: #1 total tags in treatment: 8080302 INFO @ Sat, 15 Jan 2022 22:03:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:27: #1 tags after filtering in treatment: 8080302 INFO @ Sat, 15 Jan 2022 22:03:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:03:27: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:03:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 22:03:28: 2000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:03:33: 3000000 INFO @ Sat, 15 Jan 2022 22:03:38: 4000000 INFO @ Sat, 15 Jan 2022 22:03:43: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:03:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:03:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:03:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:03:49: 6000000 INFO @ Sat, 15 Jan 2022 22:03:52: 1000000 INFO @ Sat, 15 Jan 2022 22:03:54: 7000000 INFO @ Sat, 15 Jan 2022 22:03:57: 2000000 INFO @ Sat, 15 Jan 2022 22:04:00: 8000000 INFO @ Sat, 15 Jan 2022 22:04:00: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:04:00: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:04:00: #1 total tags in treatment: 8080302 INFO @ Sat, 15 Jan 2022 22:04:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:04:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:04:00: #1 tags after filtering in treatment: 8080302 INFO @ Sat, 15 Jan 2022 22:04:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:04:00: #1 finished! INFO @ Sat, 15 Jan 2022 22:04:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:04:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:04:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:04:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:04:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:04:03: 3000000 INFO @ Sat, 15 Jan 2022 22:04:08: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:04:13: 5000000 INFO @ Sat, 15 Jan 2022 22:04:19: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:04:24: 7000000 INFO @ Sat, 15 Jan 2022 22:04:29: 8000000 INFO @ Sat, 15 Jan 2022 22:04:29: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:04:29: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:04:29: #1 total tags in treatment: 8080302 INFO @ Sat, 15 Jan 2022 22:04:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:04:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:04:30: #1 tags after filtering in treatment: 8080302 INFO @ Sat, 15 Jan 2022 22:04:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:04:30: #1 finished! INFO @ Sat, 15 Jan 2022 22:04:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:04:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:04:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:04:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:04:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829040/SRX1829040.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling