Job ID = 14522008 SRX = SRX1829039 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 23555547 spots for SRR3644962/SRR3644962.sra Written 23555547 spots for SRR3644962/SRR3644962.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:43 23555547 reads; of these: 23555547 (100.00%) were unpaired; of these: 2165316 (9.19%) aligned 0 times 18867409 (80.10%) aligned exactly 1 time 2522822 (10.71%) aligned >1 times 90.81% overall alignment rate Time searching: 00:02:43 Overall time: 00:02:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 9358167 / 21390231 = 0.4375 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:05:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:05:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:05:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:05:47: 1000000 INFO @ Sat, 15 Jan 2022 22:05:53: 2000000 INFO @ Sat, 15 Jan 2022 22:06:00: 3000000 INFO @ Sat, 15 Jan 2022 22:06:06: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:06:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:06:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:06:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:06:12: 5000000 INFO @ Sat, 15 Jan 2022 22:06:17: 1000000 INFO @ Sat, 15 Jan 2022 22:06:19: 6000000 INFO @ Sat, 15 Jan 2022 22:06:24: 2000000 INFO @ Sat, 15 Jan 2022 22:06:26: 7000000 INFO @ Sat, 15 Jan 2022 22:06:31: 3000000 INFO @ Sat, 15 Jan 2022 22:06:32: 8000000 INFO @ Sat, 15 Jan 2022 22:06:37: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:06:39: 9000000 INFO @ Sat, 15 Jan 2022 22:06:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:06:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:06:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:06:44: 5000000 INFO @ Sat, 15 Jan 2022 22:06:46: 10000000 INFO @ Sat, 15 Jan 2022 22:06:47: 1000000 INFO @ Sat, 15 Jan 2022 22:06:51: 6000000 INFO @ Sat, 15 Jan 2022 22:06:53: 11000000 INFO @ Sat, 15 Jan 2022 22:06:54: 2000000 INFO @ Sat, 15 Jan 2022 22:06:58: 7000000 INFO @ Sat, 15 Jan 2022 22:07:00: 12000000 INFO @ Sat, 15 Jan 2022 22:07:00: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:07:00: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:07:00: #1 total tags in treatment: 12032064 INFO @ Sat, 15 Jan 2022 22:07:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:07:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:07:00: #1 tags after filtering in treatment: 12032064 INFO @ Sat, 15 Jan 2022 22:07:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:07:00: #1 finished! INFO @ Sat, 15 Jan 2022 22:07:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:07:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:07:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:07:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:07:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 22:07:01: 3000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:07:04: 8000000 INFO @ Sat, 15 Jan 2022 22:07:08: 4000000 INFO @ Sat, 15 Jan 2022 22:07:11: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:07:15: 5000000 INFO @ Sat, 15 Jan 2022 22:07:18: 10000000 INFO @ Sat, 15 Jan 2022 22:07:21: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:07:25: 11000000 INFO @ Sat, 15 Jan 2022 22:07:28: 7000000 INFO @ Sat, 15 Jan 2022 22:07:31: 12000000 INFO @ Sat, 15 Jan 2022 22:07:32: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:07:32: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:07:32: #1 total tags in treatment: 12032064 INFO @ Sat, 15 Jan 2022 22:07:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:07:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:07:32: #1 tags after filtering in treatment: 12032064 INFO @ Sat, 15 Jan 2022 22:07:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:07:32: #1 finished! INFO @ Sat, 15 Jan 2022 22:07:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:07:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:07:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:07:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:07:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:07:35: 8000000 INFO @ Sat, 15 Jan 2022 22:07:41: 9000000 INFO @ Sat, 15 Jan 2022 22:07:48: 10000000 INFO @ Sat, 15 Jan 2022 22:07:54: 11000000 INFO @ Sat, 15 Jan 2022 22:08:00: 12000000 INFO @ Sat, 15 Jan 2022 22:08:00: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:08:00: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:08:00: #1 total tags in treatment: 12032064 INFO @ Sat, 15 Jan 2022 22:08:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:08:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:08:00: #1 tags after filtering in treatment: 12032064 INFO @ Sat, 15 Jan 2022 22:08:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:08:00: #1 finished! INFO @ Sat, 15 Jan 2022 22:08:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:08:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:08:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:08:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:08:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829039/SRX1829039.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling