Job ID = 14522005 SRX = SRX1829037 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 17713714 spots for SRR3644960/SRR3644960.sra Written 17713714 spots for SRR3644960/SRR3644960.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:12 17713714 reads; of these: 17713714 (100.00%) were unpaired; of these: 1376931 (7.77%) aligned 0 times 14201278 (80.17%) aligned exactly 1 time 2135505 (12.06%) aligned >1 times 92.23% overall alignment rate Time searching: 00:02:12 Overall time: 00:02:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6671415 / 16336783 = 0.4084 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:05:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:05:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:05:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:05:15: 1000000 INFO @ Sat, 15 Jan 2022 22:05:23: 2000000 INFO @ Sat, 15 Jan 2022 22:05:29: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:05:37: 4000000 INFO @ Sat, 15 Jan 2022 22:05:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:05:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:05:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:05:44: 5000000 INFO @ Sat, 15 Jan 2022 22:05:46: 1000000 INFO @ Sat, 15 Jan 2022 22:05:52: 6000000 INFO @ Sat, 15 Jan 2022 22:05:53: 2000000 INFO @ Sat, 15 Jan 2022 22:05:59: 7000000 INFO @ Sat, 15 Jan 2022 22:06:00: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:06:06: 8000000 INFO @ Sat, 15 Jan 2022 22:06:06: 4000000 INFO @ Sat, 15 Jan 2022 22:06:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:06:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:06:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:06:13: 9000000 INFO @ Sat, 15 Jan 2022 22:06:13: 5000000 INFO @ Sat, 15 Jan 2022 22:06:15: 1000000 INFO @ Sat, 15 Jan 2022 22:06:17: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:06:17: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:06:17: #1 total tags in treatment: 9665368 INFO @ Sat, 15 Jan 2022 22:06:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:06:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:06:17: #1 tags after filtering in treatment: 9665368 INFO @ Sat, 15 Jan 2022 22:06:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:06:17: #1 finished! INFO @ Sat, 15 Jan 2022 22:06:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:06:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:06:18: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:06:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:06:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:06:20: 6000000 INFO @ Sat, 15 Jan 2022 22:06:22: 2000000 INFO @ Sat, 15 Jan 2022 22:06:27: 7000000 INFO @ Sat, 15 Jan 2022 22:06:29: 3000000 INFO @ Sat, 15 Jan 2022 22:06:34: 8000000 INFO @ Sat, 15 Jan 2022 22:06:36: 4000000 INFO @ Sat, 15 Jan 2022 22:06:40: 9000000 INFO @ Sat, 15 Jan 2022 22:06:42: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:06:44: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:06:44: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:06:44: #1 total tags in treatment: 9665368 INFO @ Sat, 15 Jan 2022 22:06:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:06:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:06:45: #1 tags after filtering in treatment: 9665368 INFO @ Sat, 15 Jan 2022 22:06:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:06:45: #1 finished! INFO @ Sat, 15 Jan 2022 22:06:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:06:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:06:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:06:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:06:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:06:49: 6000000 INFO @ Sat, 15 Jan 2022 22:06:55: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:07:01: 8000000 INFO @ Sat, 15 Jan 2022 22:07:07: 9000000 INFO @ Sat, 15 Jan 2022 22:07:11: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:07:11: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:07:11: #1 total tags in treatment: 9665368 INFO @ Sat, 15 Jan 2022 22:07:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:07:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:07:11: #1 tags after filtering in treatment: 9665368 INFO @ Sat, 15 Jan 2022 22:07:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:07:11: #1 finished! INFO @ Sat, 15 Jan 2022 22:07:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:07:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:07:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:07:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:07:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829037/SRX1829037.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling