Job ID = 14522004 SRX = SRX1829036 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 19391835 spots for SRR3644959/SRR3644959.sra Written 19391835 spots for SRR3644959/SRR3644959.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:03 19391835 reads; of these: 19391835 (100.00%) were unpaired; of these: 2774873 (14.31%) aligned 0 times 14347006 (73.98%) aligned exactly 1 time 2269956 (11.71%) aligned >1 times 85.69% overall alignment rate Time searching: 00:02:03 Overall time: 00:02:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7704703 / 16616962 = 0.4637 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:03:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:03:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:03:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:03:43: 1000000 INFO @ Sat, 15 Jan 2022 22:03:48: 2000000 INFO @ Sat, 15 Jan 2022 22:03:53: 3000000 INFO @ Sat, 15 Jan 2022 22:03:59: 4000000 INFO @ Sat, 15 Jan 2022 22:04:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:04:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:04:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:04:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:04:09: 6000000 INFO @ Sat, 15 Jan 2022 22:04:13: 1000000 INFO @ Sat, 15 Jan 2022 22:04:14: 7000000 INFO @ Sat, 15 Jan 2022 22:04:17: 2000000 INFO @ Sat, 15 Jan 2022 22:04:19: 8000000 INFO @ Sat, 15 Jan 2022 22:04:22: 3000000 INFO @ Sat, 15 Jan 2022 22:04:24: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:04:24: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:04:24: #1 total tags in treatment: 8912259 INFO @ Sat, 15 Jan 2022 22:04:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:04:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:04:24: #1 tags after filtering in treatment: 8912259 INFO @ Sat, 15 Jan 2022 22:04:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:04:24: #1 finished! INFO @ Sat, 15 Jan 2022 22:04:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:04:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:04:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:04:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:04:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:04:27: 4000000 INFO @ Sat, 15 Jan 2022 22:04:31: 5000000 INFO @ Sat, 15 Jan 2022 22:04:36: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:04:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:04:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:04:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:04:40: 7000000 INFO @ Sat, 15 Jan 2022 22:04:43: 1000000 INFO @ Sat, 15 Jan 2022 22:04:45: 8000000 INFO @ Sat, 15 Jan 2022 22:04:48: 2000000 INFO @ Sat, 15 Jan 2022 22:04:49: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:04:49: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:04:49: #1 total tags in treatment: 8912259 INFO @ Sat, 15 Jan 2022 22:04:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:04:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:04:50: #1 tags after filtering in treatment: 8912259 INFO @ Sat, 15 Jan 2022 22:04:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:04:50: #1 finished! INFO @ Sat, 15 Jan 2022 22:04:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:04:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:04:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:04:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:04:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:04:53: 3000000 INFO @ Sat, 15 Jan 2022 22:04:58: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:05:03: 5000000 INFO @ Sat, 15 Jan 2022 22:05:08: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:05:13: 7000000 INFO @ Sat, 15 Jan 2022 22:05:18: 8000000 INFO @ Sat, 15 Jan 2022 22:05:22: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 22:05:22: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 22:05:22: #1 total tags in treatment: 8912259 INFO @ Sat, 15 Jan 2022 22:05:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:05:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:05:22: #1 tags after filtering in treatment: 8912259 INFO @ Sat, 15 Jan 2022 22:05:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:05:22: #1 finished! INFO @ Sat, 15 Jan 2022 22:05:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:05:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:05:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:05:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:05:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1829036/SRX1829036.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling