Job ID = 2009835 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 39,044,739 reads read : 39,044,739 reads written : 39,044,739 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR546144.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:47 39044739 reads; of these: 39044739 (100.00%) were unpaired; of these: 4855381 (12.44%) aligned 0 times 22696610 (58.13%) aligned exactly 1 time 11492748 (29.43%) aligned >1 times 87.56% overall alignment rate Time searching: 00:12:47 Overall time: 00:12:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 25052097 / 34189358 = 0.7327 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:39:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:39:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:39:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:39:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:39:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:39:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:39:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:39:18: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:39:18: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:39:26: 1000000 INFO @ Fri, 05 Jul 2019 20:39:29: 1000000 INFO @ Fri, 05 Jul 2019 20:39:29: 1000000 INFO @ Fri, 05 Jul 2019 20:39:36: 2000000 INFO @ Fri, 05 Jul 2019 20:39:40: 2000000 INFO @ Fri, 05 Jul 2019 20:39:41: 2000000 INFO @ Fri, 05 Jul 2019 20:39:45: 3000000 INFO @ Fri, 05 Jul 2019 20:39:51: 3000000 INFO @ Fri, 05 Jul 2019 20:39:51: 3000000 INFO @ Fri, 05 Jul 2019 20:39:55: 4000000 INFO @ Fri, 05 Jul 2019 20:40:01: 4000000 INFO @ Fri, 05 Jul 2019 20:40:02: 4000000 INFO @ Fri, 05 Jul 2019 20:40:05: 5000000 INFO @ Fri, 05 Jul 2019 20:40:12: 5000000 INFO @ Fri, 05 Jul 2019 20:40:13: 5000000 INFO @ Fri, 05 Jul 2019 20:40:14: 6000000 INFO @ Fri, 05 Jul 2019 20:40:24: 6000000 INFO @ Fri, 05 Jul 2019 20:40:24: 7000000 INFO @ Fri, 05 Jul 2019 20:40:24: 6000000 INFO @ Fri, 05 Jul 2019 20:40:34: 8000000 INFO @ Fri, 05 Jul 2019 20:40:35: 7000000 INFO @ Fri, 05 Jul 2019 20:40:35: 7000000 INFO @ Fri, 05 Jul 2019 20:40:43: 9000000 INFO @ Fri, 05 Jul 2019 20:40:44: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 20:40:44: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 20:40:44: #1 total tags in treatment: 9137261 INFO @ Fri, 05 Jul 2019 20:40:44: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:40:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:40:45: #1 tags after filtering in treatment: 9137261 INFO @ Fri, 05 Jul 2019 20:40:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:40:45: #1 finished! INFO @ Fri, 05 Jul 2019 20:40:45: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:40:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:40:45: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:40:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:40:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:40:46: 8000000 INFO @ Fri, 05 Jul 2019 20:40:46: 8000000 INFO @ Fri, 05 Jul 2019 20:40:57: 9000000 INFO @ Fri, 05 Jul 2019 20:40:57: 9000000 INFO @ Fri, 05 Jul 2019 20:40:58: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 20:40:58: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 20:40:58: #1 total tags in treatment: 9137261 INFO @ Fri, 05 Jul 2019 20:40:58: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:40:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:40:59: #1 tags after filtering in treatment: 9137261 INFO @ Fri, 05 Jul 2019 20:40:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:40:59: #1 finished! INFO @ Fri, 05 Jul 2019 20:40:59: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:40:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:40:59: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 20:40:59: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 20:40:59: #1 total tags in treatment: 9137261 INFO @ Fri, 05 Jul 2019 20:40:59: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:40:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:40:59: #1 tags after filtering in treatment: 9137261 INFO @ Fri, 05 Jul 2019 20:40:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:40:59: #1 finished! INFO @ Fri, 05 Jul 2019 20:40:59: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:40:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:40:59: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:40:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:40:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:41:00: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:41:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:41:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX180132/SRX180132.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。