Job ID = 9162252 sra ファイルのダウンロード中... Completed: 733933K bytes transferred in 9 seconds (636958K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 21716255 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1791115/SRR3567990.sra Written 21716255 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:09 21716255 reads; of these: 21716255 (100.00%) were unpaired; of these: 778723 (3.59%) aligned 0 times 18377894 (84.63%) aligned exactly 1 time 2559638 (11.79%) aligned >1 times 96.41% overall alignment rate Time searching: 00:04:09 Overall time: 00:04:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7791845 / 20937532 = 0.3721 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 07:11:47: # Command line: callpeak -t SRX1791115.bam -f BAM -g 12100000 -n SRX1791115.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1791115.10 # format = BAM # ChIP-seq file = ['SRX1791115.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:11:47: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:11:47: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:11:47: # Command line: callpeak -t SRX1791115.bam -f BAM -g 12100000 -n SRX1791115.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1791115.20 # format = BAM # ChIP-seq file = ['SRX1791115.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:11:47: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:11:47: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:11:47: # Command line: callpeak -t SRX1791115.bam -f BAM -g 12100000 -n SRX1791115.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1791115.05 # format = BAM # ChIP-seq file = ['SRX1791115.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:11:47: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:11:47: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:11:55: 1000000 INFO @ Wed, 28 Jun 2017 07:11:55: 1000000 INFO @ Wed, 28 Jun 2017 07:11:55: 1000000 INFO @ Wed, 28 Jun 2017 07:12:03: 2000000 INFO @ Wed, 28 Jun 2017 07:12:04: 2000000 INFO @ Wed, 28 Jun 2017 07:12:04: 2000000 INFO @ Wed, 28 Jun 2017 07:12:12: 3000000 INFO @ Wed, 28 Jun 2017 07:12:13: 3000000 INFO @ Wed, 28 Jun 2017 07:12:14: 3000000 INFO @ Wed, 28 Jun 2017 07:12:21: 4000000 INFO @ Wed, 28 Jun 2017 07:12:21: 4000000 INFO @ Wed, 28 Jun 2017 07:12:23: 4000000 INFO @ Wed, 28 Jun 2017 07:12:30: 5000000 INFO @ Wed, 28 Jun 2017 07:12:30: 5000000 INFO @ Wed, 28 Jun 2017 07:12:32: 5000000 INFO @ Wed, 28 Jun 2017 07:12:39: 6000000 INFO @ Wed, 28 Jun 2017 07:12:39: 6000000 INFO @ Wed, 28 Jun 2017 07:12:40: 6000000 INFO @ Wed, 28 Jun 2017 07:12:47: 7000000 INFO @ Wed, 28 Jun 2017 07:12:48: 7000000 INFO @ Wed, 28 Jun 2017 07:12:48: 7000000 INFO @ Wed, 28 Jun 2017 07:12:56: 8000000 INFO @ Wed, 28 Jun 2017 07:12:56: 8000000 INFO @ Wed, 28 Jun 2017 07:12:57: 8000000 INFO @ Wed, 28 Jun 2017 07:13:04: 9000000 INFO @ Wed, 28 Jun 2017 07:13:05: 9000000 INFO @ Wed, 28 Jun 2017 07:13:06: 9000000 INFO @ Wed, 28 Jun 2017 07:13:11: 10000000 INFO @ Wed, 28 Jun 2017 07:13:14: 10000000 INFO @ Wed, 28 Jun 2017 07:13:15: 10000000 INFO @ Wed, 28 Jun 2017 07:13:20: 11000000 INFO @ Wed, 28 Jun 2017 07:13:23: 11000000 INFO @ Wed, 28 Jun 2017 07:13:24: 11000000 INFO @ Wed, 28 Jun 2017 07:13:28: 12000000 INFO @ Wed, 28 Jun 2017 07:13:32: 12000000 INFO @ Wed, 28 Jun 2017 07:13:33: 12000000 INFO @ Wed, 28 Jun 2017 07:13:35: 13000000 INFO @ Wed, 28 Jun 2017 07:13:36: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:13:36: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:13:36: #1 total tags in treatment: 13145687 INFO @ Wed, 28 Jun 2017 07:13:36: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:13:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:13:36: #1 tags after filtering in treatment: 13145687 INFO @ Wed, 28 Jun 2017 07:13:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:13:36: #1 finished! INFO @ Wed, 28 Jun 2017 07:13:36: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:13:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:13:37: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:13:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:13:37: Process for pairing-model is terminated! cat: SRX1791115.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1791115.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791115.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791115.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:13:41: 13000000 INFO @ Wed, 28 Jun 2017 07:13:42: 13000000 INFO @ Wed, 28 Jun 2017 07:13:42: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:13:42: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:13:42: #1 total tags in treatment: 13145687 INFO @ Wed, 28 Jun 2017 07:13:42: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:13:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:13:42: #1 tags after filtering in treatment: 13145687 INFO @ Wed, 28 Jun 2017 07:13:42: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:13:42: #1 finished! INFO @ Wed, 28 Jun 2017 07:13:42: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:13:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:13:43: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:13:43: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:13:43: #1 total tags in treatment: 13145687 INFO @ Wed, 28 Jun 2017 07:13:43: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:13:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:13:43: #1 tags after filtering in treatment: 13145687 INFO @ Wed, 28 Jun 2017 07:13:43: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:13:43: #1 finished! INFO @ Wed, 28 Jun 2017 07:13:43: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:13:43: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:13:43: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:13:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:13:43: Process for pairing-model is terminated! cat: SRX1791115.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1791115.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791115.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791115.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:13:44: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:13:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:13:44: Process for pairing-model is terminated! cat: SRX1791115.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1791115.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791115.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791115.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。