Job ID = 9162241 sra ファイルのダウンロード中... Completed: 776457K bytes transferred in 9 seconds (658782K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 22387544 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1791105/SRR3567980.sra Written 22387544 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:02 22387544 reads; of these: 22387544 (100.00%) were unpaired; of these: 720105 (3.22%) aligned 0 times 18782238 (83.90%) aligned exactly 1 time 2885201 (12.89%) aligned >1 times 96.78% overall alignment rate Time searching: 00:05:02 Overall time: 00:05:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8264831 / 21667439 = 0.3814 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 07:07:43: # Command line: callpeak -t SRX1791105.bam -f BAM -g 12100000 -n SRX1791105.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1791105.20 # format = BAM # ChIP-seq file = ['SRX1791105.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:07:43: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:07:43: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:07:43: # Command line: callpeak -t SRX1791105.bam -f BAM -g 12100000 -n SRX1791105.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1791105.10 # format = BAM # ChIP-seq file = ['SRX1791105.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:07:43: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:07:43: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:07:43: # Command line: callpeak -t SRX1791105.bam -f BAM -g 12100000 -n SRX1791105.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1791105.05 # format = BAM # ChIP-seq file = ['SRX1791105.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:07:43: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:07:43: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:07:50: 1000000 INFO @ Wed, 28 Jun 2017 07:07:50: 1000000 INFO @ Wed, 28 Jun 2017 07:07:50: 1000000 INFO @ Wed, 28 Jun 2017 07:07:57: 2000000 INFO @ Wed, 28 Jun 2017 07:07:57: 2000000 INFO @ Wed, 28 Jun 2017 07:07:58: 2000000 INFO @ Wed, 28 Jun 2017 07:08:05: 3000000 INFO @ Wed, 28 Jun 2017 07:08:05: 3000000 INFO @ Wed, 28 Jun 2017 07:08:05: 3000000 INFO @ Wed, 28 Jun 2017 07:08:11: 4000000 INFO @ Wed, 28 Jun 2017 07:08:12: 4000000 INFO @ Wed, 28 Jun 2017 07:08:12: 4000000 INFO @ Wed, 28 Jun 2017 07:08:18: 5000000 INFO @ Wed, 28 Jun 2017 07:08:20: 5000000 INFO @ Wed, 28 Jun 2017 07:08:20: 5000000 INFO @ Wed, 28 Jun 2017 07:08:26: 6000000 INFO @ Wed, 28 Jun 2017 07:08:28: 6000000 INFO @ Wed, 28 Jun 2017 07:08:28: 6000000 INFO @ Wed, 28 Jun 2017 07:08:34: 7000000 INFO @ Wed, 28 Jun 2017 07:08:35: 7000000 INFO @ Wed, 28 Jun 2017 07:08:36: 7000000 INFO @ Wed, 28 Jun 2017 07:08:41: 8000000 INFO @ Wed, 28 Jun 2017 07:08:42: 8000000 INFO @ Wed, 28 Jun 2017 07:08:44: 8000000 INFO @ Wed, 28 Jun 2017 07:08:49: 9000000 INFO @ Wed, 28 Jun 2017 07:08:49: 9000000 INFO @ Wed, 28 Jun 2017 07:08:52: 9000000 INFO @ Wed, 28 Jun 2017 07:08:56: 10000000 INFO @ Wed, 28 Jun 2017 07:08:56: 10000000 INFO @ Wed, 28 Jun 2017 07:09:00: 10000000 INFO @ Wed, 28 Jun 2017 07:09:04: 11000000 INFO @ Wed, 28 Jun 2017 07:09:04: 11000000 INFO @ Wed, 28 Jun 2017 07:09:07: 11000000 INFO @ Wed, 28 Jun 2017 07:09:11: 12000000 INFO @ Wed, 28 Jun 2017 07:09:11: 12000000 INFO @ Wed, 28 Jun 2017 07:09:15: 12000000 INFO @ Wed, 28 Jun 2017 07:09:18: 13000000 INFO @ Wed, 28 Jun 2017 07:09:19: 13000000 INFO @ Wed, 28 Jun 2017 07:09:21: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:09:21: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:09:21: #1 total tags in treatment: 13402608 INFO @ Wed, 28 Jun 2017 07:09:21: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:09:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:09:22: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:09:22: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:09:22: #1 total tags in treatment: 13402608 INFO @ Wed, 28 Jun 2017 07:09:22: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:09:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:09:22: #1 tags after filtering in treatment: 13402608 INFO @ Wed, 28 Jun 2017 07:09:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:09:22: #1 finished! INFO @ Wed, 28 Jun 2017 07:09:22: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:09:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:09:22: #1 tags after filtering in treatment: 13402608 INFO @ Wed, 28 Jun 2017 07:09:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:09:22: #1 finished! INFO @ Wed, 28 Jun 2017 07:09:22: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:09:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:09:22: 13000000 INFO @ Wed, 28 Jun 2017 07:09:22: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:09:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:09:22: Process for pairing-model is terminated! cat: SRX1791105.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1791105.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791105.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791105.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:09:23: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:09:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:09:23: Process for pairing-model is terminated! cat: SRX1791105.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1791105.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791105.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791105.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:09:25: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:09:25: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:09:25: #1 total tags in treatment: 13402608 INFO @ Wed, 28 Jun 2017 07:09:25: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:09:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:09:25: #1 tags after filtering in treatment: 13402608 INFO @ Wed, 28 Jun 2017 07:09:25: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:09:25: #1 finished! INFO @ Wed, 28 Jun 2017 07:09:25: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:09:25: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:09:26: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:09:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:09:26: Process for pairing-model is terminated! cat: SRX1791105.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1791105.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791105.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1791105.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。