Job ID = 9162216 sra ファイルのダウンロード中... Completed: 731507K bytes transferred in 7 seconds (792136K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 24879371 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1738688/SRR3471219.sra Written 24879371 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:31 24879371 reads; of these: 24879371 (100.00%) were unpaired; of these: 3417265 (13.74%) aligned 0 times 19107355 (76.80%) aligned exactly 1 time 2354751 (9.46%) aligned >1 times 86.26% overall alignment rate Time searching: 00:05:31 Overall time: 00:05:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8589835 / 21462106 = 0.4002 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 07:02:51: # Command line: callpeak -t SRX1738688.bam -f BAM -g 12100000 -n SRX1738688.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1738688.20 # format = BAM # ChIP-seq file = ['SRX1738688.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:02:51: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:02:51: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:02:51: # Command line: callpeak -t SRX1738688.bam -f BAM -g 12100000 -n SRX1738688.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1738688.10 # format = BAM # ChIP-seq file = ['SRX1738688.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:02:51: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:02:51: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:02:51: # Command line: callpeak -t SRX1738688.bam -f BAM -g 12100000 -n SRX1738688.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1738688.05 # format = BAM # ChIP-seq file = ['SRX1738688.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:02:51: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:02:51: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:02:57: 1000000 INFO @ Wed, 28 Jun 2017 07:02:57: 1000000 INFO @ Wed, 28 Jun 2017 07:02:57: 1000000 INFO @ Wed, 28 Jun 2017 07:03:03: 2000000 INFO @ Wed, 28 Jun 2017 07:03:03: 2000000 INFO @ Wed, 28 Jun 2017 07:03:04: 2000000 INFO @ Wed, 28 Jun 2017 07:03:09: 3000000 INFO @ Wed, 28 Jun 2017 07:03:10: 3000000 INFO @ Wed, 28 Jun 2017 07:03:10: 3000000 INFO @ Wed, 28 Jun 2017 07:03:14: 4000000 INFO @ Wed, 28 Jun 2017 07:03:16: 4000000 INFO @ Wed, 28 Jun 2017 07:03:16: 4000000 INFO @ Wed, 28 Jun 2017 07:03:20: 5000000 INFO @ Wed, 28 Jun 2017 07:03:22: 5000000 INFO @ Wed, 28 Jun 2017 07:03:23: 5000000 INFO @ Wed, 28 Jun 2017 07:03:26: 6000000 INFO @ Wed, 28 Jun 2017 07:03:29: 6000000 INFO @ Wed, 28 Jun 2017 07:03:29: 6000000 INFO @ Wed, 28 Jun 2017 07:03:32: 7000000 INFO @ Wed, 28 Jun 2017 07:03:35: 7000000 INFO @ Wed, 28 Jun 2017 07:03:36: 7000000 INFO @ Wed, 28 Jun 2017 07:03:38: 8000000 INFO @ Wed, 28 Jun 2017 07:03:42: 8000000 INFO @ Wed, 28 Jun 2017 07:03:43: 8000000 INFO @ Wed, 28 Jun 2017 07:03:44: 9000000 INFO @ Wed, 28 Jun 2017 07:03:48: 9000000 INFO @ Wed, 28 Jun 2017 07:03:49: 9000000 INFO @ Wed, 28 Jun 2017 07:03:49: 10000000 INFO @ Wed, 28 Jun 2017 07:03:55: 10000000 INFO @ Wed, 28 Jun 2017 07:03:55: 11000000 INFO @ Wed, 28 Jun 2017 07:03:56: 10000000 INFO @ Wed, 28 Jun 2017 07:04:01: 12000000 INFO @ Wed, 28 Jun 2017 07:04:01: 11000000 INFO @ Wed, 28 Jun 2017 07:04:03: 11000000 INFO @ Wed, 28 Jun 2017 07:04:06: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:04:06: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:04:06: #1 total tags in treatment: 12872271 INFO @ Wed, 28 Jun 2017 07:04:06: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:04:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:04:06: #1 tags after filtering in treatment: 12872271 INFO @ Wed, 28 Jun 2017 07:04:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:04:06: #1 finished! INFO @ Wed, 28 Jun 2017 07:04:06: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:04:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:04:07: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:04:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:04:07: Process for pairing-model is terminated! cat: SRX1738688.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738688.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738688.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738688.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:04:08: 12000000 INFO @ Wed, 28 Jun 2017 07:04:09: 12000000 INFO @ Wed, 28 Jun 2017 07:04:14: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:04:14: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:04:14: #1 total tags in treatment: 12872271 INFO @ Wed, 28 Jun 2017 07:04:14: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:04:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:04:14: #1 tags after filtering in treatment: 12872271 INFO @ Wed, 28 Jun 2017 07:04:14: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:04:14: #1 finished! INFO @ Wed, 28 Jun 2017 07:04:14: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:04:14: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:04:15: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:04:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:04:15: Process for pairing-model is terminated! cat: SRX1738688.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738688.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738688.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738688.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:04:15: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:04:15: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:04:15: #1 total tags in treatment: 12872271 INFO @ Wed, 28 Jun 2017 07:04:15: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:04:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:04:15: #1 tags after filtering in treatment: 12872271 INFO @ Wed, 28 Jun 2017 07:04:15: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:04:15: #1 finished! INFO @ Wed, 28 Jun 2017 07:04:15: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:04:15: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:04:16: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:04:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:04:16: Process for pairing-model is terminated! cat: SRX1738688.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738688.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738688.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738688.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。