Job ID = 9162215 sra ファイルのダウンロード中... Completed: 1171958K bytes transferred in 12 seconds (781850K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 40474935 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1738687/SRR3471218.sra Written 40474935 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:21 40474935 reads; of these: 40474935 (100.00%) were unpaired; of these: 1472453 (3.64%) aligned 0 times 31119390 (76.89%) aligned exactly 1 time 7883092 (19.48%) aligned >1 times 96.36% overall alignment rate Time searching: 00:08:21 Overall time: 00:08:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 22022671 / 39002482 = 0.5646 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 07:09:32: # Command line: callpeak -t SRX1738687.bam -f BAM -g 12100000 -n SRX1738687.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1738687.20 # format = BAM # ChIP-seq file = ['SRX1738687.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:09:32: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:09:32: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:09:32: # Command line: callpeak -t SRX1738687.bam -f BAM -g 12100000 -n SRX1738687.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1738687.10 # format = BAM # ChIP-seq file = ['SRX1738687.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:09:32: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:09:32: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:09:32: # Command line: callpeak -t SRX1738687.bam -f BAM -g 12100000 -n SRX1738687.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1738687.05 # format = BAM # ChIP-seq file = ['SRX1738687.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:09:32: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:09:32: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:09:40: 1000000 INFO @ Wed, 28 Jun 2017 07:09:40: 1000000 INFO @ Wed, 28 Jun 2017 07:09:40: 1000000 INFO @ Wed, 28 Jun 2017 07:09:47: 2000000 INFO @ Wed, 28 Jun 2017 07:09:48: 2000000 INFO @ Wed, 28 Jun 2017 07:09:48: 2000000 INFO @ Wed, 28 Jun 2017 07:09:54: 3000000 INFO @ Wed, 28 Jun 2017 07:09:55: 3000000 INFO @ Wed, 28 Jun 2017 07:09:55: 3000000 INFO @ Wed, 28 Jun 2017 07:10:01: 4000000 INFO @ Wed, 28 Jun 2017 07:10:02: 4000000 INFO @ Wed, 28 Jun 2017 07:10:02: 4000000 INFO @ Wed, 28 Jun 2017 07:10:09: 5000000 INFO @ Wed, 28 Jun 2017 07:10:10: 5000000 INFO @ Wed, 28 Jun 2017 07:10:10: 5000000 INFO @ Wed, 28 Jun 2017 07:10:16: 6000000 INFO @ Wed, 28 Jun 2017 07:10:17: 6000000 INFO @ Wed, 28 Jun 2017 07:10:17: 6000000 INFO @ Wed, 28 Jun 2017 07:10:23: 7000000 INFO @ Wed, 28 Jun 2017 07:10:25: 7000000 INFO @ Wed, 28 Jun 2017 07:10:25: 7000000 INFO @ Wed, 28 Jun 2017 07:10:30: 8000000 INFO @ Wed, 28 Jun 2017 07:10:33: 8000000 INFO @ Wed, 28 Jun 2017 07:10:33: 8000000 INFO @ Wed, 28 Jun 2017 07:10:37: 9000000 INFO @ Wed, 28 Jun 2017 07:10:41: 9000000 INFO @ Wed, 28 Jun 2017 07:10:41: 9000000 INFO @ Wed, 28 Jun 2017 07:10:44: 10000000 INFO @ Wed, 28 Jun 2017 07:10:49: 10000000 INFO @ Wed, 28 Jun 2017 07:10:49: 10000000 INFO @ Wed, 28 Jun 2017 07:10:51: 11000000 INFO @ Wed, 28 Jun 2017 07:10:57: 11000000 INFO @ Wed, 28 Jun 2017 07:10:57: 11000000 INFO @ Wed, 28 Jun 2017 07:10:58: 12000000 INFO @ Wed, 28 Jun 2017 07:11:04: 12000000 INFO @ Wed, 28 Jun 2017 07:11:04: 12000000 INFO @ Wed, 28 Jun 2017 07:11:06: 13000000 INFO @ Wed, 28 Jun 2017 07:11:11: 13000000 INFO @ Wed, 28 Jun 2017 07:11:12: 13000000 INFO @ Wed, 28 Jun 2017 07:11:13: 14000000 INFO @ Wed, 28 Jun 2017 07:11:18: 14000000 INFO @ Wed, 28 Jun 2017 07:11:20: 14000000 INFO @ Wed, 28 Jun 2017 07:11:21: 15000000 INFO @ Wed, 28 Jun 2017 07:11:26: 15000000 INFO @ Wed, 28 Jun 2017 07:11:27: 15000000 INFO @ Wed, 28 Jun 2017 07:11:29: 16000000 INFO @ Wed, 28 Jun 2017 07:11:33: 16000000 INFO @ Wed, 28 Jun 2017 07:11:35: 16000000 INFO @ Wed, 28 Jun 2017 07:11:36: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:11:36: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:11:36: #1 total tags in treatment: 16979811 INFO @ Wed, 28 Jun 2017 07:11:36: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:11:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:11:36: #1 tags after filtering in treatment: 16979811 INFO @ Wed, 28 Jun 2017 07:11:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:11:36: #1 finished! INFO @ Wed, 28 Jun 2017 07:11:36: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:11:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:11:37: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:11:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:11:37: Process for pairing-model is terminated! cat: SRX1738687.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738687.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738687.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738687.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:11:40: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:11:40: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:11:40: #1 total tags in treatment: 16979811 INFO @ Wed, 28 Jun 2017 07:11:40: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:11:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:11:40: #1 tags after filtering in treatment: 16979811 INFO @ Wed, 28 Jun 2017 07:11:40: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:11:40: #1 finished! INFO @ Wed, 28 Jun 2017 07:11:40: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:11:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:11:42: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:11:42: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:11:42: #1 total tags in treatment: 16979811 INFO @ Wed, 28 Jun 2017 07:11:42: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:11:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:11:42: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:11:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:11:42: Process for pairing-model is terminated! cat: SRX1738687.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738687.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738687.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738687.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:11:42: #1 tags after filtering in treatment: 16979811 INFO @ Wed, 28 Jun 2017 07:11:42: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:11:42: #1 finished! INFO @ Wed, 28 Jun 2017 07:11:42: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:11:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:11:43: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:11:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:11:43: Process for pairing-model is terminated! cat: SRX1738687.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738687.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738687.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738687.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。