Job ID = 9162210 sra ファイルのダウンロード中... Completed: 1139173K bytes transferred in 12 seconds (729451K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 37079416 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1738682/SRR3471213.sra Written 37079416 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:51 37079416 reads; of these: 37079416 (100.00%) were unpaired; of these: 1561273 (4.21%) aligned 0 times 29537733 (79.66%) aligned exactly 1 time 5980410 (16.13%) aligned >1 times 95.79% overall alignment rate Time searching: 00:07:51 Overall time: 00:07:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18806108 / 35518143 = 0.5295 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 07:08:00: # Command line: callpeak -t SRX1738682.bam -f BAM -g 12100000 -n SRX1738682.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1738682.05 # format = BAM # ChIP-seq file = ['SRX1738682.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:08:00: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:08:00: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:08:00: # Command line: callpeak -t SRX1738682.bam -f BAM -g 12100000 -n SRX1738682.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1738682.20 # format = BAM # ChIP-seq file = ['SRX1738682.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:08:00: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:08:00: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:08:00: # Command line: callpeak -t SRX1738682.bam -f BAM -g 12100000 -n SRX1738682.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1738682.10 # format = BAM # ChIP-seq file = ['SRX1738682.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:08:00: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:08:00: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:08:07: 1000000 INFO @ Wed, 28 Jun 2017 07:08:07: 1000000 INFO @ Wed, 28 Jun 2017 07:08:07: 1000000 INFO @ Wed, 28 Jun 2017 07:08:14: 2000000 INFO @ Wed, 28 Jun 2017 07:08:14: 2000000 INFO @ Wed, 28 Jun 2017 07:08:14: 2000000 INFO @ Wed, 28 Jun 2017 07:08:21: 3000000 INFO @ Wed, 28 Jun 2017 07:08:21: 3000000 INFO @ Wed, 28 Jun 2017 07:08:21: 3000000 INFO @ Wed, 28 Jun 2017 07:08:27: 4000000 INFO @ Wed, 28 Jun 2017 07:08:29: 4000000 INFO @ Wed, 28 Jun 2017 07:08:29: 4000000 INFO @ Wed, 28 Jun 2017 07:08:34: 5000000 INFO @ Wed, 28 Jun 2017 07:08:36: 5000000 INFO @ Wed, 28 Jun 2017 07:08:36: 5000000 INFO @ Wed, 28 Jun 2017 07:08:41: 6000000 INFO @ Wed, 28 Jun 2017 07:08:43: 6000000 INFO @ Wed, 28 Jun 2017 07:08:43: 6000000 INFO @ Wed, 28 Jun 2017 07:08:48: 7000000 INFO @ Wed, 28 Jun 2017 07:08:50: 7000000 INFO @ Wed, 28 Jun 2017 07:08:50: 7000000 INFO @ Wed, 28 Jun 2017 07:08:55: 8000000 INFO @ Wed, 28 Jun 2017 07:08:57: 8000000 INFO @ Wed, 28 Jun 2017 07:08:57: 8000000 INFO @ Wed, 28 Jun 2017 07:09:02: 9000000 INFO @ Wed, 28 Jun 2017 07:09:05: 9000000 INFO @ Wed, 28 Jun 2017 07:09:05: 9000000 INFO @ Wed, 28 Jun 2017 07:09:09: 10000000 INFO @ Wed, 28 Jun 2017 07:09:12: 10000000 INFO @ Wed, 28 Jun 2017 07:09:12: 10000000 INFO @ Wed, 28 Jun 2017 07:09:16: 11000000 INFO @ Wed, 28 Jun 2017 07:09:20: 11000000 INFO @ Wed, 28 Jun 2017 07:09:20: 11000000 INFO @ Wed, 28 Jun 2017 07:09:23: 12000000 INFO @ Wed, 28 Jun 2017 07:09:27: 12000000 INFO @ Wed, 28 Jun 2017 07:09:27: 12000000 INFO @ Wed, 28 Jun 2017 07:09:30: 13000000 INFO @ Wed, 28 Jun 2017 07:09:34: 13000000 INFO @ Wed, 28 Jun 2017 07:09:34: 13000000 INFO @ Wed, 28 Jun 2017 07:09:37: 14000000 INFO @ Wed, 28 Jun 2017 07:09:41: 14000000 INFO @ Wed, 28 Jun 2017 07:09:41: 14000000 INFO @ Wed, 28 Jun 2017 07:09:43: 15000000 INFO @ Wed, 28 Jun 2017 07:09:48: 15000000 INFO @ Wed, 28 Jun 2017 07:09:48: 15000000 INFO @ Wed, 28 Jun 2017 07:09:50: 16000000 INFO @ Wed, 28 Jun 2017 07:09:55: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:09:55: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:09:55: #1 total tags in treatment: 16712035 INFO @ Wed, 28 Jun 2017 07:09:55: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:09:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:09:55: 16000000 INFO @ Wed, 28 Jun 2017 07:09:55: 16000000 INFO @ Wed, 28 Jun 2017 07:09:56: #1 tags after filtering in treatment: 16712035 INFO @ Wed, 28 Jun 2017 07:09:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:09:56: #1 finished! INFO @ Wed, 28 Jun 2017 07:09:56: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:09:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:09:56: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:09:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:09:56: Process for pairing-model is terminated! cat: SRX1738682.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738682.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738682.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738682.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:10:00: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:10:00: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:10:00: #1 total tags in treatment: 16712035 INFO @ Wed, 28 Jun 2017 07:10:00: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:10:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:10:00: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:10:00: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:10:00: #1 total tags in treatment: 16712035 INFO @ Wed, 28 Jun 2017 07:10:00: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:10:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:10:01: #1 tags after filtering in treatment: 16712035 INFO @ Wed, 28 Jun 2017 07:10:01: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:10:01: #1 finished! INFO @ Wed, 28 Jun 2017 07:10:01: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:10:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:10:01: #1 tags after filtering in treatment: 16712035 INFO @ Wed, 28 Jun 2017 07:10:01: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:10:01: #1 finished! INFO @ Wed, 28 Jun 2017 07:10:01: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:10:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:10:02: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:10:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:10:02: Process for pairing-model is terminated! cat: SRX1738682.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Wed, 28 Jun 2017 07:10:02: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:10:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:10:02: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738682.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738682.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738682.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX1738682.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738682.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738682.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738682.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。