Job ID = 2009821 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,365,346 reads read : 3,365,346 reads written : 3,365,346 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR3471157.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:27 3365346 reads; of these: 3365346 (100.00%) were unpaired; of these: 2045478 (60.78%) aligned 0 times 1189795 (35.35%) aligned exactly 1 time 130073 (3.87%) aligned >1 times 39.22% overall alignment rate Time searching: 00:00:27 Overall time: 00:00:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 81384 / 1319868 = 0.0617 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:03:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:03:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:03:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:03:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:03:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:03:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:03:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:03:18: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:03:18: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:03:25: 1000000 INFO @ Fri, 05 Jul 2019 20:03:25: 1000000 INFO @ Fri, 05 Jul 2019 20:03:25: 1000000 INFO @ Fri, 05 Jul 2019 20:03:26: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:03:26: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:03:26: #1 total tags in treatment: 1238484 INFO @ Fri, 05 Jul 2019 20:03:26: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:03:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:03:26: #1 tags after filtering in treatment: 1238484 INFO @ Fri, 05 Jul 2019 20:03:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:03:26: #1 finished! INFO @ Fri, 05 Jul 2019 20:03:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:03:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:03:27: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:03:27: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:03:27: #1 total tags in treatment: 1238484 INFO @ Fri, 05 Jul 2019 20:03:27: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:03:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:03:27: #2 number of paired peaks: 29 WARNING @ Fri, 05 Jul 2019 20:03:27: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:03:27: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 20:03:27: #1 tags after filtering in treatment: 1238484 INFO @ Fri, 05 Jul 2019 20:03:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:03:27: #1 finished! INFO @ Fri, 05 Jul 2019 20:03:27: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:03:27: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:03:27: #2 number of paired peaks: 29 WARNING @ Fri, 05 Jul 2019 20:03:27: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:03:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:03:27: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:03:27: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:03:27: #1 total tags in treatment: 1238484 INFO @ Fri, 05 Jul 2019 20:03:27: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:03:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:03:27: #1 tags after filtering in treatment: 1238484 INFO @ Fri, 05 Jul 2019 20:03:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:03:27: #1 finished! INFO @ Fri, 05 Jul 2019 20:03:27: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:03:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:03:27: #2 number of paired peaks: 29 WARNING @ Fri, 05 Jul 2019 20:03:27: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:03:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1738626/SRX1738626.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。