Job ID = 2009806 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 39,793,825 reads read : 39,793,825 reads written : 39,793,825 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:15 39793825 reads; of these: 39793825 (100.00%) were unpaired; of these: 2475855 (6.22%) aligned 0 times 30137104 (75.73%) aligned exactly 1 time 7180866 (18.05%) aligned >1 times 93.78% overall alignment rate Time searching: 00:05:15 Overall time: 00:05:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 21804487 / 37317970 = 0.5843 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:28:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:28:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:28:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:28:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:28:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:28:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:28:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:28:18: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:28:18: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:28:24: 1000000 INFO @ Fri, 05 Jul 2019 20:28:25: 1000000 INFO @ Fri, 05 Jul 2019 20:28:27: 1000000 INFO @ Fri, 05 Jul 2019 20:28:32: 2000000 INFO @ Fri, 05 Jul 2019 20:28:33: 2000000 INFO @ Fri, 05 Jul 2019 20:28:36: 2000000 INFO @ Fri, 05 Jul 2019 20:28:39: 3000000 INFO @ Fri, 05 Jul 2019 20:28:40: 3000000 INFO @ Fri, 05 Jul 2019 20:28:45: 3000000 INFO @ Fri, 05 Jul 2019 20:28:47: 4000000 INFO @ Fri, 05 Jul 2019 20:28:48: 4000000 INFO @ Fri, 05 Jul 2019 20:28:53: 4000000 INFO @ Fri, 05 Jul 2019 20:28:54: 5000000 INFO @ Fri, 05 Jul 2019 20:28:55: 5000000 INFO @ Fri, 05 Jul 2019 20:29:00: 5000000 INFO @ Fri, 05 Jul 2019 20:29:02: 6000000 INFO @ Fri, 05 Jul 2019 20:29:05: 6000000 INFO @ Fri, 05 Jul 2019 20:29:08: 6000000 INFO @ Fri, 05 Jul 2019 20:29:09: 7000000 INFO @ Fri, 05 Jul 2019 20:29:12: 7000000 INFO @ Fri, 05 Jul 2019 20:29:15: 7000000 INFO @ Fri, 05 Jul 2019 20:29:17: 8000000 INFO @ Fri, 05 Jul 2019 20:29:19: 8000000 INFO @ Fri, 05 Jul 2019 20:29:22: 8000000 INFO @ Fri, 05 Jul 2019 20:29:24: 9000000 INFO @ Fri, 05 Jul 2019 20:29:27: 9000000 INFO @ Fri, 05 Jul 2019 20:29:30: 9000000 INFO @ Fri, 05 Jul 2019 20:29:31: 10000000 INFO @ Fri, 05 Jul 2019 20:29:34: 10000000 INFO @ Fri, 05 Jul 2019 20:29:37: 10000000 INFO @ Fri, 05 Jul 2019 20:29:38: 11000000 INFO @ Fri, 05 Jul 2019 20:29:41: 11000000 INFO @ Fri, 05 Jul 2019 20:29:44: 11000000 INFO @ Fri, 05 Jul 2019 20:29:46: 12000000 INFO @ Fri, 05 Jul 2019 20:29:48: 12000000 INFO @ Fri, 05 Jul 2019 20:29:51: 12000000 INFO @ Fri, 05 Jul 2019 20:29:53: 13000000 INFO @ Fri, 05 Jul 2019 20:29:56: 13000000 INFO @ Fri, 05 Jul 2019 20:29:59: 13000000 INFO @ Fri, 05 Jul 2019 20:30:00: 14000000 INFO @ Fri, 05 Jul 2019 20:30:03: 14000000 INFO @ Fri, 05 Jul 2019 20:30:06: 14000000 INFO @ Fri, 05 Jul 2019 20:30:08: 15000000 INFO @ Fri, 05 Jul 2019 20:30:10: 15000000 INFO @ Fri, 05 Jul 2019 20:30:11: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:30:11: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:30:11: #1 total tags in treatment: 15513483 INFO @ Fri, 05 Jul 2019 20:30:11: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:30:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:30:12: #1 tags after filtering in treatment: 15513483 INFO @ Fri, 05 Jul 2019 20:30:12: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:30:12: #1 finished! INFO @ Fri, 05 Jul 2019 20:30:12: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:30:12: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:30:13: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:30:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:30:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:30:13: 15000000 INFO @ Fri, 05 Jul 2019 20:30:14: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:30:14: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:30:14: #1 total tags in treatment: 15513483 INFO @ Fri, 05 Jul 2019 20:30:14: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:30:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:30:14: #1 tags after filtering in treatment: 15513483 INFO @ Fri, 05 Jul 2019 20:30:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:30:14: #1 finished! INFO @ Fri, 05 Jul 2019 20:30:14: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:30:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:30:16: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:30:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:30:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:30:17: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:30:17: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:30:17: #1 total tags in treatment: 15513483 INFO @ Fri, 05 Jul 2019 20:30:17: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:30:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:30:17: #1 tags after filtering in treatment: 15513483 INFO @ Fri, 05 Jul 2019 20:30:17: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:30:17: #1 finished! INFO @ Fri, 05 Jul 2019 20:30:17: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:30:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:30:18: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:30:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:30:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX170680/SRX170680.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。