Job ID = 9162151 sra ファイルのダウンロード中... Completed: 1750268K bytes transferred in 16 seconds (878151K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 28485376 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1702430/SRR3375895.sra Written 28485376 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:04 28485376 reads; of these: 28485376 (100.00%) were unpaired; of these: 1661521 (5.83%) aligned 0 times 24517632 (86.07%) aligned exactly 1 time 2306223 (8.10%) aligned >1 times 94.17% overall alignment rate Time searching: 00:12:04 Overall time: 00:12:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 19220469 / 26823855 = 0.7165 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 06:47:58: # Command line: callpeak -t SRX1702430.bam -f BAM -g 12100000 -n SRX1702430.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1702430.20 # format = BAM # ChIP-seq file = ['SRX1702430.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:47:58: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:47:58: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:47:58: # Command line: callpeak -t SRX1702430.bam -f BAM -g 12100000 -n SRX1702430.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1702430.05 # format = BAM # ChIP-seq file = ['SRX1702430.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:47:58: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:47:58: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:47:58: # Command line: callpeak -t SRX1702430.bam -f BAM -g 12100000 -n SRX1702430.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1702430.10 # format = BAM # ChIP-seq file = ['SRX1702430.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:47:58: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:47:58: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:48:07: 1000000 INFO @ Wed, 28 Jun 2017 06:48:07: 1000000 INFO @ Wed, 28 Jun 2017 06:48:07: 1000000 INFO @ Wed, 28 Jun 2017 06:48:18: 2000000 INFO @ Wed, 28 Jun 2017 06:48:18: 2000000 INFO @ Wed, 28 Jun 2017 06:48:18: 2000000 INFO @ Wed, 28 Jun 2017 06:48:30: 3000000 INFO @ Wed, 28 Jun 2017 06:48:30: 3000000 INFO @ Wed, 28 Jun 2017 06:48:30: 3000000 INFO @ Wed, 28 Jun 2017 06:48:40: 4000000 INFO @ Wed, 28 Jun 2017 06:48:40: 4000000 INFO @ Wed, 28 Jun 2017 06:48:42: 4000000 INFO @ Wed, 28 Jun 2017 06:48:49: 5000000 INFO @ Wed, 28 Jun 2017 06:48:49: 5000000 INFO @ Wed, 28 Jun 2017 06:48:53: 5000000 INFO @ Wed, 28 Jun 2017 06:48:57: 6000000 INFO @ Wed, 28 Jun 2017 06:48:57: 6000000 INFO @ Wed, 28 Jun 2017 06:49:04: 6000000 INFO @ Wed, 28 Jun 2017 06:49:05: 7000000 INFO @ Wed, 28 Jun 2017 06:49:06: 7000000 INFO @ Wed, 28 Jun 2017 06:49:10: #1 tag size is determined as 147 bps INFO @ Wed, 28 Jun 2017 06:49:10: #1 tag size = 147 INFO @ Wed, 28 Jun 2017 06:49:10: #1 total tags in treatment: 7603386 INFO @ Wed, 28 Jun 2017 06:49:10: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:49:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:49:10: #1 tags after filtering in treatment: 7603386 INFO @ Wed, 28 Jun 2017 06:49:10: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 06:49:10: #1 finished! INFO @ Wed, 28 Jun 2017 06:49:10: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:49:10: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:49:11: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 06:49:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:49:11: Process for pairing-model is terminated! cat: SRX1702430.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1702430.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1702430.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1702430.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 06:49:11: #1 tag size is determined as 147 bps INFO @ Wed, 28 Jun 2017 06:49:11: #1 tag size = 147 INFO @ Wed, 28 Jun 2017 06:49:11: #1 total tags in treatment: 7603386 INFO @ Wed, 28 Jun 2017 06:49:11: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:49:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:49:11: #1 tags after filtering in treatment: 7603386 INFO @ Wed, 28 Jun 2017 06:49:11: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 06:49:11: #1 finished! INFO @ Wed, 28 Jun 2017 06:49:11: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:49:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:49:12: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 06:49:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:49:12: Process for pairing-model is terminated! cat: SRX1702430.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1702430.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1702430.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1702430.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 06:49:14: 7000000 INFO @ Wed, 28 Jun 2017 06:49:19: #1 tag size is determined as 147 bps INFO @ Wed, 28 Jun 2017 06:49:19: #1 tag size = 147 INFO @ Wed, 28 Jun 2017 06:49:19: #1 total tags in treatment: 7603386 INFO @ Wed, 28 Jun 2017 06:49:19: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:49:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:49:19: #1 tags after filtering in treatment: 7603386 INFO @ Wed, 28 Jun 2017 06:49:19: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 06:49:19: #1 finished! INFO @ Wed, 28 Jun 2017 06:49:19: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:49:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:49:20: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 06:49:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:49:20: Process for pairing-model is terminated! cat: SRX1702430.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1702430.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1702430.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1702430.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。