Job ID = 9162123 sra ファイルのダウンロード中... Completed: 612663K bytes transferred in 7 seconds (652789K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Written 4159097 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1639662/SRR3234025.sra Written 4159097 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:56 4159097 reads; of these: 4159097 (100.00%) were paired; of these: 776390 (18.67%) aligned concordantly 0 times 2787977 (67.03%) aligned concordantly exactly 1 time 594730 (14.30%) aligned concordantly >1 times ---- 776390 pairs aligned concordantly 0 times; of these: 40623 (5.23%) aligned discordantly 1 time ---- 735767 pairs aligned 0 times concordantly or discordantly; of these: 1471534 mates make up the pairs; of these: 1417738 (96.34%) aligned 0 times 30417 (2.07%) aligned exactly 1 time 23379 (1.59%) aligned >1 times 82.96% overall alignment rate Time searching: 00:05:56 Overall time: 00:05:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 499412 / 1323873 = 0.3772 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 06:06:40: # Command line: callpeak -t SRX1639662.bam -f BAM -g 12100000 -n SRX1639662.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1639662.20 # format = BAM # ChIP-seq file = ['SRX1639662.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:06:40: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:06:40: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:06:40: # Command line: callpeak -t SRX1639662.bam -f BAM -g 12100000 -n SRX1639662.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1639662.10 # format = BAM # ChIP-seq file = ['SRX1639662.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:06:40: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:06:40: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:06:40: # Command line: callpeak -t SRX1639662.bam -f BAM -g 12100000 -n SRX1639662.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1639662.05 # format = BAM # ChIP-seq file = ['SRX1639662.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:06:40: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:06:40: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:06:48: 1000000 INFO @ Wed, 28 Jun 2017 06:06:48: 1000000 INFO @ Wed, 28 Jun 2017 06:06:48: 1000000 INFO @ Wed, 28 Jun 2017 06:06:56: 2000000 INFO @ Wed, 28 Jun 2017 06:06:56: 2000000 INFO @ Wed, 28 Jun 2017 06:06:57: 2000000 INFO @ Wed, 28 Jun 2017 06:07:03: 3000000 INFO @ Wed, 28 Jun 2017 06:07:05: 3000000 INFO @ Wed, 28 Jun 2017 06:07:06: 3000000 INFO @ Wed, 28 Jun 2017 06:07:11: 4000000 INFO @ Wed, 28 Jun 2017 06:07:13: 4000000 INFO @ Wed, 28 Jun 2017 06:07:15: 4000000 INFO @ Wed, 28 Jun 2017 06:07:18: 5000000 INFO @ Wed, 28 Jun 2017 06:07:22: 5000000 INFO @ Wed, 28 Jun 2017 06:07:24: 5000000 INFO @ Wed, 28 Jun 2017 06:07:25: #1 tag size is determined as 129 bps INFO @ Wed, 28 Jun 2017 06:07:25: #1 tag size = 129 INFO @ Wed, 28 Jun 2017 06:07:25: #1 total tags in treatment: 2886450 INFO @ Wed, 28 Jun 2017 06:07:25: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:07:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:07:25: #1 tags after filtering in treatment: 1768046 INFO @ Wed, 28 Jun 2017 06:07:25: #1 Redundant rate of treatment: 0.39 INFO @ Wed, 28 Jun 2017 06:07:25: #1 finished! INFO @ Wed, 28 Jun 2017 06:07:25: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:07:25: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:07:25: #2 number of paired peaks: 49 WARNING @ Wed, 28 Jun 2017 06:07:25: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:07:25: Process for pairing-model is terminated! cat: SRX1639662.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1639662.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1639662.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1639662.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 06:07:30: #1 tag size is determined as 129 bps INFO @ Wed, 28 Jun 2017 06:07:30: #1 tag size = 129 INFO @ Wed, 28 Jun 2017 06:07:30: #1 total tags in treatment: 2886450 INFO @ Wed, 28 Jun 2017 06:07:30: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:07:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:07:30: #1 tags after filtering in treatment: 1768046 INFO @ Wed, 28 Jun 2017 06:07:30: #1 Redundant rate of treatment: 0.39 INFO @ Wed, 28 Jun 2017 06:07:30: #1 finished! INFO @ Wed, 28 Jun 2017 06:07:30: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:07:30: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:07:30: #2 number of paired peaks: 49 WARNING @ Wed, 28 Jun 2017 06:07:30: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:07:30: Process for pairing-model is terminated! cat: SRX1639662.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1639662.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1639662.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1639662.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 06:07:31: #1 tag size is determined as 129 bps INFO @ Wed, 28 Jun 2017 06:07:31: #1 tag size = 129 INFO @ Wed, 28 Jun 2017 06:07:31: #1 total tags in treatment: 2886450 INFO @ Wed, 28 Jun 2017 06:07:31: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:07:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:07:31: #1 tags after filtering in treatment: 1768046 INFO @ Wed, 28 Jun 2017 06:07:31: #1 Redundant rate of treatment: 0.39 INFO @ Wed, 28 Jun 2017 06:07:31: #1 finished! INFO @ Wed, 28 Jun 2017 06:07:31: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:07:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:07:32: #2 number of paired peaks: 49 WARNING @ Wed, 28 Jun 2017 06:07:32: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:07:32: Process for pairing-model is terminated! cat: SRX1639662.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1639662.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1639662.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1639662.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。