Job ID = 2009782 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 20,822,804 reads read : 20,822,804 reads written : 20,822,804 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:18 20822804 reads; of these: 20822804 (100.00%) were unpaired; of these: 7482495 (35.93%) aligned 0 times 11519212 (55.32%) aligned exactly 1 time 1821097 (8.75%) aligned >1 times 64.07% overall alignment rate Time searching: 00:04:18 Overall time: 00:04:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 6202215 / 13340309 = 0.4649 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:06:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:06:13: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:06:13: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:06:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:06:14: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:06:14: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:06:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:06:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:06:15: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:06:24: 1000000 INFO @ Fri, 05 Jul 2019 20:06:24: 1000000 INFO @ Fri, 05 Jul 2019 20:06:26: 1000000 INFO @ Fri, 05 Jul 2019 20:06:33: 2000000 INFO @ Fri, 05 Jul 2019 20:06:35: 2000000 INFO @ Fri, 05 Jul 2019 20:06:37: 2000000 INFO @ Fri, 05 Jul 2019 20:06:42: 3000000 INFO @ Fri, 05 Jul 2019 20:06:45: 3000000 INFO @ Fri, 05 Jul 2019 20:06:47: 3000000 INFO @ Fri, 05 Jul 2019 20:06:51: 4000000 INFO @ Fri, 05 Jul 2019 20:06:55: 4000000 INFO @ Fri, 05 Jul 2019 20:06:57: 4000000 INFO @ Fri, 05 Jul 2019 20:07:00: 5000000 INFO @ Fri, 05 Jul 2019 20:07:05: 5000000 INFO @ Fri, 05 Jul 2019 20:07:07: 5000000 INFO @ Fri, 05 Jul 2019 20:07:09: 6000000 INFO @ Fri, 05 Jul 2019 20:07:16: 6000000 INFO @ Fri, 05 Jul 2019 20:07:18: 7000000 INFO @ Fri, 05 Jul 2019 20:07:18: 6000000 INFO @ Fri, 05 Jul 2019 20:07:19: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:07:19: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:07:19: #1 total tags in treatment: 7138094 INFO @ Fri, 05 Jul 2019 20:07:19: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:07:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:07:19: #1 tags after filtering in treatment: 7138094 INFO @ Fri, 05 Jul 2019 20:07:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:07:19: #1 finished! INFO @ Fri, 05 Jul 2019 20:07:19: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:07:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:07:20: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:07:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:07:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:07:26: 7000000 INFO @ Fri, 05 Jul 2019 20:07:27: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:07:27: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:07:27: #1 total tags in treatment: 7138094 INFO @ Fri, 05 Jul 2019 20:07:27: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:07:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:07:27: #1 tags after filtering in treatment: 7138094 INFO @ Fri, 05 Jul 2019 20:07:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:07:27: #1 finished! INFO @ Fri, 05 Jul 2019 20:07:27: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:07:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:07:27: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:07:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:07:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:07:28: 7000000 INFO @ Fri, 05 Jul 2019 20:07:29: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:07:29: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:07:29: #1 total tags in treatment: 7138094 INFO @ Fri, 05 Jul 2019 20:07:29: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:07:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:07:29: #1 tags after filtering in treatment: 7138094 INFO @ Fri, 05 Jul 2019 20:07:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:07:29: #1 finished! INFO @ Fri, 05 Jul 2019 20:07:29: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:07:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:07:30: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:07:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:07:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150901/SRX150901.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。