Job ID = 2009776 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 24,362,916 reads read : 24,362,916 reads written : 24,362,916 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR502960.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:55 24362916 reads; of these: 24362916 (100.00%) were unpaired; of these: 4628945 (19.00%) aligned 0 times 17559765 (72.08%) aligned exactly 1 time 2174206 (8.92%) aligned >1 times 81.00% overall alignment rate Time searching: 00:03:55 Overall time: 00:03:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10329384 / 19733971 = 0.5234 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:00:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:00:11: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:00:11: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:00:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:00:12: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:00:12: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:00:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:00:13: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:00:13: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:00:19: 1000000 INFO @ Fri, 05 Jul 2019 20:00:21: 1000000 INFO @ Fri, 05 Jul 2019 20:00:21: 1000000 INFO @ Fri, 05 Jul 2019 20:00:28: 2000000 INFO @ Fri, 05 Jul 2019 20:00:28: 2000000 INFO @ Fri, 05 Jul 2019 20:00:30: 2000000 INFO @ Fri, 05 Jul 2019 20:00:36: 3000000 INFO @ Fri, 05 Jul 2019 20:00:36: 3000000 INFO @ Fri, 05 Jul 2019 20:00:39: 3000000 INFO @ Fri, 05 Jul 2019 20:00:43: 4000000 INFO @ Fri, 05 Jul 2019 20:00:44: 4000000 INFO @ Fri, 05 Jul 2019 20:00:47: 4000000 INFO @ Fri, 05 Jul 2019 20:00:51: 5000000 INFO @ Fri, 05 Jul 2019 20:00:52: 5000000 INFO @ Fri, 05 Jul 2019 20:00:56: 5000000 INFO @ Fri, 05 Jul 2019 20:00:58: 6000000 INFO @ Fri, 05 Jul 2019 20:00:59: 6000000 INFO @ Fri, 05 Jul 2019 20:01:04: 6000000 INFO @ Fri, 05 Jul 2019 20:01:06: 7000000 INFO @ Fri, 05 Jul 2019 20:01:07: 7000000 INFO @ Fri, 05 Jul 2019 20:01:12: 7000000 INFO @ Fri, 05 Jul 2019 20:01:13: 8000000 INFO @ Fri, 05 Jul 2019 20:01:15: 8000000 INFO @ Fri, 05 Jul 2019 20:01:21: 9000000 INFO @ Fri, 05 Jul 2019 20:01:21: 8000000 INFO @ Fri, 05 Jul 2019 20:01:23: 9000000 INFO @ Fri, 05 Jul 2019 20:01:24: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:01:24: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:01:24: #1 total tags in treatment: 9404587 INFO @ Fri, 05 Jul 2019 20:01:24: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:01:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:01:24: #1 tags after filtering in treatment: 9404587 INFO @ Fri, 05 Jul 2019 20:01:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:01:24: #1 finished! INFO @ Fri, 05 Jul 2019 20:01:24: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:01:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:01:25: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:01:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:01:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:01:26: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:01:26: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:01:26: #1 total tags in treatment: 9404587 INFO @ Fri, 05 Jul 2019 20:01:26: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:01:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:01:26: #1 tags after filtering in treatment: 9404587 INFO @ Fri, 05 Jul 2019 20:01:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:01:26: #1 finished! INFO @ Fri, 05 Jul 2019 20:01:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:01:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:01:27: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:01:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:01:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:01:29: 9000000 INFO @ Fri, 05 Jul 2019 20:01:32: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:01:32: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:01:32: #1 total tags in treatment: 9404587 INFO @ Fri, 05 Jul 2019 20:01:32: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:01:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:01:33: #1 tags after filtering in treatment: 9404587 INFO @ Fri, 05 Jul 2019 20:01:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:01:33: #1 finished! INFO @ Fri, 05 Jul 2019 20:01:33: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:01:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:01:33: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:01:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:01:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150896/SRX150896.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。