Job ID = 2009774 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 25,444,278 reads read : 25,444,278 reads written : 25,444,278 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR502959.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:03 25444278 reads; of these: 25444278 (100.00%) were unpaired; of these: 7209912 (28.34%) aligned 0 times 16078215 (63.19%) aligned exactly 1 time 2156151 (8.47%) aligned >1 times 71.66% overall alignment rate Time searching: 00:03:03 Overall time: 00:03:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10059925 / 18234366 = 0.5517 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:58:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:58:20: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:58:20: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:58:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:58:21: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:58:21: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:58:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:58:22: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:58:22: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:58:27: 1000000 INFO @ Fri, 05 Jul 2019 19:58:28: 1000000 INFO @ Fri, 05 Jul 2019 19:58:30: 1000000 INFO @ Fri, 05 Jul 2019 19:58:34: 2000000 INFO @ Fri, 05 Jul 2019 19:58:35: 2000000 INFO @ Fri, 05 Jul 2019 19:58:38: 2000000 INFO @ Fri, 05 Jul 2019 19:58:40: 3000000 INFO @ Fri, 05 Jul 2019 19:58:41: 3000000 INFO @ Fri, 05 Jul 2019 19:58:46: 3000000 INFO @ Fri, 05 Jul 2019 19:58:47: 4000000 INFO @ Fri, 05 Jul 2019 19:58:48: 4000000 INFO @ Fri, 05 Jul 2019 19:58:54: 4000000 INFO @ Fri, 05 Jul 2019 19:58:54: 5000000 INFO @ Fri, 05 Jul 2019 19:58:55: 5000000 INFO @ Fri, 05 Jul 2019 19:59:00: 6000000 INFO @ Fri, 05 Jul 2019 19:59:01: 5000000 INFO @ Fri, 05 Jul 2019 19:59:01: 6000000 INFO @ Fri, 05 Jul 2019 19:59:07: 7000000 INFO @ Fri, 05 Jul 2019 19:59:08: 7000000 INFO @ Fri, 05 Jul 2019 19:59:09: 6000000 INFO @ Fri, 05 Jul 2019 19:59:14: 8000000 INFO @ Fri, 05 Jul 2019 19:59:15: 8000000 INFO @ Fri, 05 Jul 2019 19:59:15: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:59:15: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:59:15: #1 total tags in treatment: 8174441 INFO @ Fri, 05 Jul 2019 19:59:15: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:59:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:59:15: #1 tags after filtering in treatment: 8174441 INFO @ Fri, 05 Jul 2019 19:59:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:59:15: #1 finished! INFO @ Fri, 05 Jul 2019 19:59:15: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:59:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:59:16: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:59:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:59:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:59:16: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:59:16: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:59:16: #1 total tags in treatment: 8174441 INFO @ Fri, 05 Jul 2019 19:59:16: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:59:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:59:16: #1 tags after filtering in treatment: 8174441 INFO @ Fri, 05 Jul 2019 19:59:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:59:16: #1 finished! INFO @ Fri, 05 Jul 2019 19:59:16: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:59:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:59:17: 7000000 INFO @ Fri, 05 Jul 2019 19:59:17: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:59:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:59:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:59:24: 8000000 INFO @ Fri, 05 Jul 2019 19:59:25: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:59:25: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:59:25: #1 total tags in treatment: 8174441 INFO @ Fri, 05 Jul 2019 19:59:25: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:59:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:59:26: #1 tags after filtering in treatment: 8174441 INFO @ Fri, 05 Jul 2019 19:59:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:59:26: #1 finished! INFO @ Fri, 05 Jul 2019 19:59:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:59:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:59:26: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:59:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:59:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX150895/SRX150895.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。