Job ID = 2009766 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 125,250,963 reads read : 125,250,963 reads written : 125,250,963 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR500629.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:46 125250963 reads; of these: 125250963 (100.00%) were unpaired; of these: 57470141 (45.88%) aligned 0 times 52136229 (41.63%) aligned exactly 1 time 15644593 (12.49%) aligned >1 times 54.12% overall alignment rate Time searching: 00:19:46 Overall time: 00:19:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 55277277 / 67780822 = 0.8155 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:38:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:38:19: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:38:19: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:38:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:38:20: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:38:20: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:38:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:38:21: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:38:21: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:38:30: 1000000 INFO @ Fri, 05 Jul 2019 20:38:32: 1000000 INFO @ Fri, 05 Jul 2019 20:38:33: 1000000 INFO @ Fri, 05 Jul 2019 20:38:42: 2000000 INFO @ Fri, 05 Jul 2019 20:38:43: 2000000 INFO @ Fri, 05 Jul 2019 20:38:44: 2000000 INFO @ Fri, 05 Jul 2019 20:38:53: 3000000 INFO @ Fri, 05 Jul 2019 20:38:54: 3000000 INFO @ Fri, 05 Jul 2019 20:38:55: 3000000 INFO @ Fri, 05 Jul 2019 20:39:03: 4000000 INFO @ Fri, 05 Jul 2019 20:39:05: 4000000 INFO @ Fri, 05 Jul 2019 20:39:06: 4000000 INFO @ Fri, 05 Jul 2019 20:39:14: 5000000 INFO @ Fri, 05 Jul 2019 20:39:16: 5000000 INFO @ Fri, 05 Jul 2019 20:39:17: 5000000 INFO @ Fri, 05 Jul 2019 20:39:25: 6000000 INFO @ Fri, 05 Jul 2019 20:39:27: 6000000 INFO @ Fri, 05 Jul 2019 20:39:27: 6000000 INFO @ Fri, 05 Jul 2019 20:39:36: 7000000 INFO @ Fri, 05 Jul 2019 20:39:38: 7000000 INFO @ Fri, 05 Jul 2019 20:39:38: 7000000 INFO @ Fri, 05 Jul 2019 20:39:46: 8000000 INFO @ Fri, 05 Jul 2019 20:39:49: 8000000 INFO @ Fri, 05 Jul 2019 20:39:49: 8000000 INFO @ Fri, 05 Jul 2019 20:39:57: 9000000 INFO @ Fri, 05 Jul 2019 20:40:00: 9000000 INFO @ Fri, 05 Jul 2019 20:40:00: 9000000 INFO @ Fri, 05 Jul 2019 20:40:08: 10000000 INFO @ Fri, 05 Jul 2019 20:40:11: 10000000 INFO @ Fri, 05 Jul 2019 20:40:11: 10000000 INFO @ Fri, 05 Jul 2019 20:40:18: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 20:40:22: 11000000 INFO @ Fri, 05 Jul 2019 20:40:22: 11000000 INFO @ Fri, 05 Jul 2019 20:40:29: 12000000 INFO @ Fri, 05 Jul 2019 20:40:33: 12000000 INFO @ Fri, 05 Jul 2019 20:40:33: 12000000 BigWig に変換しました。 INFO @ Fri, 05 Jul 2019 20:40:34: #1 tag size is determined as 59 bps INFO @ Fri, 05 Jul 2019 20:40:34: #1 tag size = 59 INFO @ Fri, 05 Jul 2019 20:40:34: #1 total tags in treatment: 12503545 INFO @ Fri, 05 Jul 2019 20:40:34: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:40:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:40:35: #1 tags after filtering in treatment: 12503545 INFO @ Fri, 05 Jul 2019 20:40:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:40:35: #1 finished! INFO @ Fri, 05 Jul 2019 20:40:35: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:40:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:40:36: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:40:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:40:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:40:38: #1 tag size is determined as 59 bps INFO @ Fri, 05 Jul 2019 20:40:38: #1 tag size = 59 INFO @ Fri, 05 Jul 2019 20:40:38: #1 total tags in treatment: 12503545 INFO @ Fri, 05 Jul 2019 20:40:38: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:40:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:40:38: #1 tags after filtering in treatment: 12503545 INFO @ Fri, 05 Jul 2019 20:40:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:40:38: #1 finished! INFO @ Fri, 05 Jul 2019 20:40:38: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:40:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:40:38: #1 tag size is determined as 59 bps INFO @ Fri, 05 Jul 2019 20:40:38: #1 tag size = 59 INFO @ Fri, 05 Jul 2019 20:40:38: #1 total tags in treatment: 12503545 INFO @ Fri, 05 Jul 2019 20:40:38: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:40:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:40:38: #1 tags after filtering in treatment: 12503545 INFO @ Fri, 05 Jul 2019 20:40:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:40:38: #1 finished! INFO @ Fri, 05 Jul 2019 20:40:38: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:40:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:40:39: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:40:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:40:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:40:39: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:40:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:40:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX149466/SRX149466.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling