Job ID = 9162020 sra ファイルのダウンロード中... Completed: 1368736K bytes transferred in 15 seconds (701104K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 56845905 spots for /home/okishinya/chipatlas/results/sacCer3/SRX149455/SRR500618.sra Written 56845905 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:53 56845905 reads; of these: 56845905 (100.00%) were unpaired; of these: 22453696 (39.50%) aligned 0 times 28887263 (50.82%) aligned exactly 1 time 5504946 (9.68%) aligned >1 times 60.50% overall alignment rate Time searching: 00:10:53 Overall time: 00:10:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 21069344 / 34392209 = 0.6126 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 05:48:07: # Command line: callpeak -t SRX149455.bam -f BAM -g 12100000 -n SRX149455.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX149455.20 # format = BAM # ChIP-seq file = ['SRX149455.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:48:07: # Command line: callpeak -t SRX149455.bam -f BAM -g 12100000 -n SRX149455.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX149455.05 # format = BAM # ChIP-seq file = ['SRX149455.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:48:07: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:48:07: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:48:07: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:48:07: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:48:07: # Command line: callpeak -t SRX149455.bam -f BAM -g 12100000 -n SRX149455.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX149455.10 # format = BAM # ChIP-seq file = ['SRX149455.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:48:07: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:48:07: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:48:14: 1000000 INFO @ Wed, 28 Jun 2017 05:48:14: 1000000 INFO @ Wed, 28 Jun 2017 05:48:14: 1000000 INFO @ Wed, 28 Jun 2017 05:48:20: 2000000 INFO @ Wed, 28 Jun 2017 05:48:21: 2000000 INFO @ Wed, 28 Jun 2017 05:48:21: 2000000 INFO @ Wed, 28 Jun 2017 05:48:27: 3000000 INFO @ Wed, 28 Jun 2017 05:48:28: 3000000 INFO @ Wed, 28 Jun 2017 05:48:29: 3000000 INFO @ Wed, 28 Jun 2017 05:48:33: 4000000 INFO @ Wed, 28 Jun 2017 05:48:35: 4000000 INFO @ Wed, 28 Jun 2017 05:48:36: 4000000 INFO @ Wed, 28 Jun 2017 05:48:39: 5000000 INFO @ Wed, 28 Jun 2017 05:48:43: 5000000 INFO @ Wed, 28 Jun 2017 05:48:44: 5000000 INFO @ Wed, 28 Jun 2017 05:48:46: 6000000 INFO @ Wed, 28 Jun 2017 05:48:50: 6000000 INFO @ Wed, 28 Jun 2017 05:48:51: 6000000 INFO @ Wed, 28 Jun 2017 05:48:53: 7000000 INFO @ Wed, 28 Jun 2017 05:48:58: 7000000 INFO @ Wed, 28 Jun 2017 05:48:59: 7000000 INFO @ Wed, 28 Jun 2017 05:49:00: 8000000 INFO @ Wed, 28 Jun 2017 05:49:05: 8000000 INFO @ Wed, 28 Jun 2017 05:49:07: 8000000 INFO @ Wed, 28 Jun 2017 05:49:07: 9000000 INFO @ Wed, 28 Jun 2017 05:49:12: 9000000 INFO @ Wed, 28 Jun 2017 05:49:14: 9000000 INFO @ Wed, 28 Jun 2017 05:49:14: 10000000 INFO @ Wed, 28 Jun 2017 05:49:19: 10000000 INFO @ Wed, 28 Jun 2017 05:49:21: 10000000 INFO @ Wed, 28 Jun 2017 05:49:22: 11000000 INFO @ Wed, 28 Jun 2017 05:49:26: 11000000 INFO @ Wed, 28 Jun 2017 05:49:29: 11000000 INFO @ Wed, 28 Jun 2017 05:49:29: 12000000 INFO @ Wed, 28 Jun 2017 05:49:34: 12000000 INFO @ Wed, 28 Jun 2017 05:49:36: 12000000 INFO @ Wed, 28 Jun 2017 05:49:37: 13000000 INFO @ Wed, 28 Jun 2017 05:49:39: #1 tag size is determined as 39 bps INFO @ Wed, 28 Jun 2017 05:49:39: #1 tag size = 39 INFO @ Wed, 28 Jun 2017 05:49:39: #1 total tags in treatment: 13322865 INFO @ Wed, 28 Jun 2017 05:49:39: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:49:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:49:39: #1 tags after filtering in treatment: 13322865 INFO @ Wed, 28 Jun 2017 05:49:39: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:49:39: #1 finished! INFO @ Wed, 28 Jun 2017 05:49:39: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:49:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:49:40: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:49:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:49:40: Process for pairing-model is terminated! cat: SRX149455.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX149455.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149455.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149455.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 05:49:41: 13000000 INFO @ Wed, 28 Jun 2017 05:49:43: #1 tag size is determined as 39 bps INFO @ Wed, 28 Jun 2017 05:49:43: #1 tag size = 39 INFO @ Wed, 28 Jun 2017 05:49:43: #1 total tags in treatment: 13322865 INFO @ Wed, 28 Jun 2017 05:49:43: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:49:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:49:43: 13000000 INFO @ Wed, 28 Jun 2017 05:49:43: #1 tags after filtering in treatment: 13322865 INFO @ Wed, 28 Jun 2017 05:49:43: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:49:43: #1 finished! INFO @ Wed, 28 Jun 2017 05:49:43: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:49:43: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:49:44: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:49:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:49:44: Process for pairing-model is terminated! cat: SRX149455.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX149455.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149455.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149455.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 05:49:45: #1 tag size is determined as 39 bps INFO @ Wed, 28 Jun 2017 05:49:45: #1 tag size = 39 INFO @ Wed, 28 Jun 2017 05:49:45: #1 total tags in treatment: 13322865 INFO @ Wed, 28 Jun 2017 05:49:45: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:49:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:49:46: #1 tags after filtering in treatment: 13322865 INFO @ Wed, 28 Jun 2017 05:49:46: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:49:46: #1 finished! INFO @ Wed, 28 Jun 2017 05:49:46: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:49:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:49:46: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:49:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:49:46: Process for pairing-model is terminated! cat: SRX149455.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX149455.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149455.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149455.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。