Job ID = 9162019 sra ファイルのダウンロード中... Completed: 4496047K bytes transferred in 42 seconds (874832K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 123076523 spots for /home/okishinya/chipatlas/results/sacCer3/SRX149454/SRR500617.sra Written 123076523 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:06 123076523 reads; of these: 123076523 (100.00%) were unpaired; of these: 14874824 (12.09%) aligned 0 times 88896743 (72.23%) aligned exactly 1 time 19304956 (15.69%) aligned >1 times 87.91% overall alignment rate Time searching: 00:26:06 Overall time: 00:26:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 92825695 / 108201699 = 0.8579 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 06:21:21: # Command line: callpeak -t SRX149454.bam -f BAM -g 12100000 -n SRX149454.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX149454.05 # format = BAM # ChIP-seq file = ['SRX149454.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:21:21: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:21:21: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:21:21: # Command line: callpeak -t SRX149454.bam -f BAM -g 12100000 -n SRX149454.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX149454.20 # format = BAM # ChIP-seq file = ['SRX149454.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:21:21: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:21:21: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:21:21: # Command line: callpeak -t SRX149454.bam -f BAM -g 12100000 -n SRX149454.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX149454.10 # format = BAM # ChIP-seq file = ['SRX149454.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:21:21: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:21:21: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:21:29: 1000000 INFO @ Wed, 28 Jun 2017 06:21:29: 1000000 INFO @ Wed, 28 Jun 2017 06:21:29: 1000000 INFO @ Wed, 28 Jun 2017 06:21:37: 2000000 INFO @ Wed, 28 Jun 2017 06:21:37: 2000000 INFO @ Wed, 28 Jun 2017 06:21:37: 2000000 INFO @ Wed, 28 Jun 2017 06:21:44: 3000000 INFO @ Wed, 28 Jun 2017 06:21:44: 3000000 INFO @ Wed, 28 Jun 2017 06:21:44: 3000000 INFO @ Wed, 28 Jun 2017 06:21:51: 4000000 INFO @ Wed, 28 Jun 2017 06:21:51: 4000000 INFO @ Wed, 28 Jun 2017 06:21:51: 4000000 INFO @ Wed, 28 Jun 2017 06:21:59: 5000000 INFO @ Wed, 28 Jun 2017 06:21:59: 5000000 INFO @ Wed, 28 Jun 2017 06:21:59: 5000000 INFO @ Wed, 28 Jun 2017 06:22:06: 6000000 INFO @ Wed, 28 Jun 2017 06:22:06: 6000000 INFO @ Wed, 28 Jun 2017 06:22:06: 6000000 INFO @ Wed, 28 Jun 2017 06:22:14: 7000000 INFO @ Wed, 28 Jun 2017 06:22:14: 7000000 INFO @ Wed, 28 Jun 2017 06:22:14: 7000000 INFO @ Wed, 28 Jun 2017 06:22:21: 8000000 INFO @ Wed, 28 Jun 2017 06:22:21: 8000000 INFO @ Wed, 28 Jun 2017 06:22:21: 8000000 INFO @ Wed, 28 Jun 2017 06:22:29: 9000000 INFO @ Wed, 28 Jun 2017 06:22:29: 9000000 INFO @ Wed, 28 Jun 2017 06:22:29: 9000000 INFO @ Wed, 28 Jun 2017 06:22:36: 10000000 INFO @ Wed, 28 Jun 2017 06:22:36: 10000000 INFO @ Wed, 28 Jun 2017 06:22:36: 10000000 INFO @ Wed, 28 Jun 2017 06:22:43: 11000000 INFO @ Wed, 28 Jun 2017 06:22:43: 11000000 INFO @ Wed, 28 Jun 2017 06:22:43: 11000000 INFO @ Wed, 28 Jun 2017 06:22:50: 12000000 INFO @ Wed, 28 Jun 2017 06:22:51: 12000000 INFO @ Wed, 28 Jun 2017 06:22:51: 12000000 INFO @ Wed, 28 Jun 2017 06:22:58: 13000000 INFO @ Wed, 28 Jun 2017 06:22:58: 13000000 INFO @ Wed, 28 Jun 2017 06:22:58: 13000000 INFO @ Wed, 28 Jun 2017 06:23:05: 14000000 INFO @ Wed, 28 Jun 2017 06:23:06: 14000000 INFO @ Wed, 28 Jun 2017 06:23:06: 14000000 INFO @ Wed, 28 Jun 2017 06:23:13: 15000000 INFO @ Wed, 28 Jun 2017 06:23:13: 15000000 INFO @ Wed, 28 Jun 2017 06:23:14: 15000000 INFO @ Wed, 28 Jun 2017 06:23:16: #1 tag size is determined as 59 bps INFO @ Wed, 28 Jun 2017 06:23:16: #1 tag size = 59 INFO @ Wed, 28 Jun 2017 06:23:16: #1 total tags in treatment: 15376004 INFO @ Wed, 28 Jun 2017 06:23:16: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:23:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:23:16: #1 tags after filtering in treatment: 15376004 INFO @ Wed, 28 Jun 2017 06:23:16: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 06:23:16: #1 finished! INFO @ Wed, 28 Jun 2017 06:23:16: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:23:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:23:16: #1 tag size is determined as 59 bps INFO @ Wed, 28 Jun 2017 06:23:16: #1 tag size = 59 INFO @ Wed, 28 Jun 2017 06:23:16: #1 total tags in treatment: 15376004 INFO @ Wed, 28 Jun 2017 06:23:16: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:23:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:23:16: #1 tags after filtering in treatment: 15376004 INFO @ Wed, 28 Jun 2017 06:23:16: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 06:23:16: #1 finished! INFO @ Wed, 28 Jun 2017 06:23:16: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:23:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:23:17: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 06:23:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:23:17: Process for pairing-model is terminated! cat: SRX149454.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX149454.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149454.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149454.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 06:23:17: #1 tag size is determined as 59 bps INFO @ Wed, 28 Jun 2017 06:23:17: #1 tag size = 59 INFO @ Wed, 28 Jun 2017 06:23:17: #1 total tags in treatment: 15376004 INFO @ Wed, 28 Jun 2017 06:23:17: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:23:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:23:17: #1 tags after filtering in treatment: 15376004 INFO @ Wed, 28 Jun 2017 06:23:17: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 06:23:17: #1 finished! INFO @ Wed, 28 Jun 2017 06:23:17: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:23:17: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:23:17: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 06:23:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:23:17: Process for pairing-model is terminated! cat: SRX149454.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX149454.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149454.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149454.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 06:23:18: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 06:23:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:23:18: Process for pairing-model is terminated! cat: SRX149454.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX149454.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149454.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149454.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。