Job ID = 9162016 sra ファイルのダウンロード中... Completed: 4885853K bytes transferred in 43 seconds (915905K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 129534801 spots for /home/okishinya/chipatlas/results/sacCer3/SRX149453/SRR500616.sra Written 129534801 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:49 129534801 reads; of these: 129534801 (100.00%) were unpaired; of these: 16463219 (12.71%) aligned 0 times 90858061 (70.14%) aligned exactly 1 time 22213521 (17.15%) aligned >1 times 87.29% overall alignment rate Time searching: 00:29:49 Overall time: 00:29:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 96018254 / 113071582 = 0.8492 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 06:27:18: # Command line: callpeak -t SRX149453.bam -f BAM -g 12100000 -n SRX149453.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX149453.10 # format = BAM # ChIP-seq file = ['SRX149453.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:27:18: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:27:18: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:27:18: # Command line: callpeak -t SRX149453.bam -f BAM -g 12100000 -n SRX149453.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX149453.20 # format = BAM # ChIP-seq file = ['SRX149453.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:27:18: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:27:18: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:27:18: # Command line: callpeak -t SRX149453.bam -f BAM -g 12100000 -n SRX149453.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX149453.05 # format = BAM # ChIP-seq file = ['SRX149453.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 06:27:18: #1 read tag files... INFO @ Wed, 28 Jun 2017 06:27:18: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 06:27:27: 1000000 INFO @ Wed, 28 Jun 2017 06:27:27: 1000000 INFO @ Wed, 28 Jun 2017 06:27:27: 1000000 INFO @ Wed, 28 Jun 2017 06:27:37: 2000000 INFO @ Wed, 28 Jun 2017 06:27:37: 2000000 INFO @ Wed, 28 Jun 2017 06:27:37: 2000000 INFO @ Wed, 28 Jun 2017 06:27:47: 3000000 INFO @ Wed, 28 Jun 2017 06:27:47: 3000000 INFO @ Wed, 28 Jun 2017 06:27:47: 3000000 INFO @ Wed, 28 Jun 2017 06:27:56: 4000000 INFO @ Wed, 28 Jun 2017 06:27:56: 4000000 INFO @ Wed, 28 Jun 2017 06:27:56: 4000000 INFO @ Wed, 28 Jun 2017 06:28:06: 5000000 INFO @ Wed, 28 Jun 2017 06:28:06: 5000000 INFO @ Wed, 28 Jun 2017 06:28:06: 5000000 INFO @ Wed, 28 Jun 2017 06:28:16: 6000000 INFO @ Wed, 28 Jun 2017 06:28:16: 6000000 INFO @ Wed, 28 Jun 2017 06:28:16: 6000000 INFO @ Wed, 28 Jun 2017 06:28:25: 7000000 INFO @ Wed, 28 Jun 2017 06:28:26: 7000000 INFO @ Wed, 28 Jun 2017 06:28:26: 7000000 INFO @ Wed, 28 Jun 2017 06:28:35: 8000000 INFO @ Wed, 28 Jun 2017 06:28:35: 8000000 INFO @ Wed, 28 Jun 2017 06:28:36: 8000000 INFO @ Wed, 28 Jun 2017 06:28:45: 9000000 INFO @ Wed, 28 Jun 2017 06:28:46: 9000000 INFO @ Wed, 28 Jun 2017 06:28:46: 9000000 INFO @ Wed, 28 Jun 2017 06:28:56: 10000000 INFO @ Wed, 28 Jun 2017 06:28:56: 10000000 INFO @ Wed, 28 Jun 2017 06:28:56: 10000000 INFO @ Wed, 28 Jun 2017 06:29:06: 11000000 INFO @ Wed, 28 Jun 2017 06:29:06: 11000000 INFO @ Wed, 28 Jun 2017 06:29:06: 11000000 INFO @ Wed, 28 Jun 2017 06:29:16: 12000000 INFO @ Wed, 28 Jun 2017 06:29:16: 12000000 INFO @ Wed, 28 Jun 2017 06:29:16: 12000000 INFO @ Wed, 28 Jun 2017 06:29:26: 13000000 INFO @ Wed, 28 Jun 2017 06:29:26: 13000000 INFO @ Wed, 28 Jun 2017 06:29:26: 13000000 INFO @ Wed, 28 Jun 2017 06:29:36: 14000000 INFO @ Wed, 28 Jun 2017 06:29:36: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 28 Jun 2017 06:29:36: 14000000 INFO @ Wed, 28 Jun 2017 06:29:46: 15000000 INFO @ Wed, 28 Jun 2017 06:29:46: 15000000 INFO @ Wed, 28 Jun 2017 06:29:47: 15000000 BigWig に変換しました。 INFO @ Wed, 28 Jun 2017 06:29:55: 16000000 INFO @ Wed, 28 Jun 2017 06:29:55: 16000000 INFO @ Wed, 28 Jun 2017 06:29:57: 16000000 INFO @ Wed, 28 Jun 2017 06:30:04: 17000000 INFO @ Wed, 28 Jun 2017 06:30:04: 17000000 INFO @ Wed, 28 Jun 2017 06:30:05: #1 tag size is determined as 59 bps INFO @ Wed, 28 Jun 2017 06:30:05: #1 tag size = 59 INFO @ Wed, 28 Jun 2017 06:30:05: #1 total tags in treatment: 17053328 INFO @ Wed, 28 Jun 2017 06:30:05: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:30:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:30:05: #1 tag size is determined as 59 bps INFO @ Wed, 28 Jun 2017 06:30:05: #1 tag size = 59 INFO @ Wed, 28 Jun 2017 06:30:05: #1 total tags in treatment: 17053328 INFO @ Wed, 28 Jun 2017 06:30:05: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:30:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:30:05: #1 tags after filtering in treatment: 17053328 INFO @ Wed, 28 Jun 2017 06:30:05: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 06:30:05: #1 finished! INFO @ Wed, 28 Jun 2017 06:30:05: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:30:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:30:05: #1 tags after filtering in treatment: 17053328 INFO @ Wed, 28 Jun 2017 06:30:05: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 06:30:05: #1 finished! INFO @ Wed, 28 Jun 2017 06:30:05: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:30:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:30:06: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 06:30:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:30:06: Process for pairing-model is terminated! cat: SRX149453.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Wed, 28 Jun 2017 06:30:06: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 06:30:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:30:06: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 1 millis cat: SRX149453.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX149453.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149453.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149453.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX149453.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149453.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149453.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 06:30:07: 17000000 INFO @ Wed, 28 Jun 2017 06:30:07: #1 tag size is determined as 59 bps INFO @ Wed, 28 Jun 2017 06:30:07: #1 tag size = 59 INFO @ Wed, 28 Jun 2017 06:30:07: #1 total tags in treatment: 17053328 INFO @ Wed, 28 Jun 2017 06:30:07: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 06:30:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 06:30:08: #1 tags after filtering in treatment: 17053328 INFO @ Wed, 28 Jun 2017 06:30:08: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 06:30:08: #1 finished! INFO @ Wed, 28 Jun 2017 06:30:08: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 06:30:08: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 06:30:09: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 06:30:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 06:30:09: Process for pairing-model is terminated! cat: SRX149453.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX149453.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149453.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX149453.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling