Job ID = 2009760 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,837,675 reads read : 8,837,675 reads written : 8,837,675 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR497937.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:06 8837675 reads; of these: 8837675 (100.00%) were unpaired; of these: 1351475 (15.29%) aligned 0 times 5936798 (67.18%) aligned exactly 1 time 1549402 (17.53%) aligned >1 times 84.71% overall alignment rate Time searching: 00:02:06 Overall time: 00:02:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3367153 / 7486200 = 0.4498 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:50:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:50:52: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:50:52: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:50:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:50:52: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:50:52: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:50:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:50:54: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:50:54: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:50:59: 1000000 INFO @ Fri, 05 Jul 2019 19:51:00: 1000000 INFO @ Fri, 05 Jul 2019 19:51:02: 1000000 INFO @ Fri, 05 Jul 2019 19:51:06: 2000000 INFO @ Fri, 05 Jul 2019 19:51:07: 2000000 INFO @ Fri, 05 Jul 2019 19:51:10: 2000000 INFO @ Fri, 05 Jul 2019 19:51:13: 3000000 INFO @ Fri, 05 Jul 2019 19:51:14: 3000000 INFO @ Fri, 05 Jul 2019 19:51:19: 3000000 INFO @ Fri, 05 Jul 2019 19:51:20: 4000000 INFO @ Fri, 05 Jul 2019 19:51:21: #1 tag size is determined as 30 bps INFO @ Fri, 05 Jul 2019 19:51:21: #1 tag size = 30 INFO @ Fri, 05 Jul 2019 19:51:21: #1 total tags in treatment: 4119047 INFO @ Fri, 05 Jul 2019 19:51:21: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:51:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:51:21: #1 tags after filtering in treatment: 4119047 INFO @ Fri, 05 Jul 2019 19:51:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:51:21: #1 finished! INFO @ Fri, 05 Jul 2019 19:51:21: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:51:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:51:21: 4000000 INFO @ Fri, 05 Jul 2019 19:51:21: #2 number of paired peaks: 30 WARNING @ Fri, 05 Jul 2019 19:51:21: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:51:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:51:22: #1 tag size is determined as 30 bps INFO @ Fri, 05 Jul 2019 19:51:22: #1 tag size = 30 INFO @ Fri, 05 Jul 2019 19:51:22: #1 total tags in treatment: 4119047 INFO @ Fri, 05 Jul 2019 19:51:22: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:51:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:51:22: #1 tags after filtering in treatment: 4119047 INFO @ Fri, 05 Jul 2019 19:51:22: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:51:22: #1 finished! INFO @ Fri, 05 Jul 2019 19:51:22: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:51:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:51:22: #2 number of paired peaks: 30 WARNING @ Fri, 05 Jul 2019 19:51:22: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:51:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:51:27: 4000000 INFO @ Fri, 05 Jul 2019 19:51:28: #1 tag size is determined as 30 bps INFO @ Fri, 05 Jul 2019 19:51:28: #1 tag size = 30 INFO @ Fri, 05 Jul 2019 19:51:28: #1 total tags in treatment: 4119047 INFO @ Fri, 05 Jul 2019 19:51:28: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:51:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:51:28: #1 tags after filtering in treatment: 4119047 INFO @ Fri, 05 Jul 2019 19:51:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:51:28: #1 finished! INFO @ Fri, 05 Jul 2019 19:51:28: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:51:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:51:28: #2 number of paired peaks: 30 WARNING @ Fri, 05 Jul 2019 19:51:28: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:51:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX148616/SRX148616.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。