Job ID = 9161930 sra ファイルのダウンロード中... Completed: 225213K bytes transferred in 4 seconds (369953K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Written 3461539 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1419781/SRR2927530.sra Written 3461539 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:55 3461539 reads; of these: 3461539 (100.00%) were paired; of these: 219678 (6.35%) aligned concordantly 0 times 2863709 (82.73%) aligned concordantly exactly 1 time 378152 (10.92%) aligned concordantly >1 times ---- 219678 pairs aligned concordantly 0 times; of these: 55406 (25.22%) aligned discordantly 1 time ---- 164272 pairs aligned 0 times concordantly or discordantly; of these: 328544 mates make up the pairs; of these: 284737 (86.67%) aligned 0 times 20326 (6.19%) aligned exactly 1 time 23481 (7.15%) aligned >1 times 95.89% overall alignment rate Time searching: 00:03:55 Overall time: 00:03:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 176983 / 3287782 = 0.0538 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 05:06:33: # Command line: callpeak -t SRX1419781.bam -f BAM -g 12100000 -n SRX1419781.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1419781.10 # format = BAM # ChIP-seq file = ['SRX1419781.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:06:33: # Command line: callpeak -t SRX1419781.bam -f BAM -g 12100000 -n SRX1419781.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1419781.05 # format = BAM # ChIP-seq file = ['SRX1419781.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:06:33: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:06:33: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:06:33: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:06:33: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:06:33: # Command line: callpeak -t SRX1419781.bam -f BAM -g 12100000 -n SRX1419781.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1419781.20 # format = BAM # ChIP-seq file = ['SRX1419781.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:06:33: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:06:33: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:06:41: 1000000 INFO @ Wed, 28 Jun 2017 05:06:42: 1000000 INFO @ Wed, 28 Jun 2017 05:06:42: 1000000 INFO @ Wed, 28 Jun 2017 05:06:48: 2000000 INFO @ Wed, 28 Jun 2017 05:06:51: 2000000 INFO @ Wed, 28 Jun 2017 05:06:51: 2000000 INFO @ Wed, 28 Jun 2017 05:06:56: 3000000 INFO @ Wed, 28 Jun 2017 05:06:58: 3000000 INFO @ Wed, 28 Jun 2017 05:06:58: 3000000 INFO @ Wed, 28 Jun 2017 05:07:03: 4000000 INFO @ Wed, 28 Jun 2017 05:07:05: 4000000 INFO @ Wed, 28 Jun 2017 05:07:05: 4000000 INFO @ Wed, 28 Jun 2017 05:07:10: 5000000 INFO @ Wed, 28 Jun 2017 05:07:12: 5000000 INFO @ Wed, 28 Jun 2017 05:07:12: 5000000 INFO @ Wed, 28 Jun 2017 05:07:17: 6000000 INFO @ Wed, 28 Jun 2017 05:07:19: #1 tag size is determined as 94 bps INFO @ Wed, 28 Jun 2017 05:07:19: #1 tag size = 94 INFO @ Wed, 28 Jun 2017 05:07:19: #1 total tags in treatment: 3066504 INFO @ Wed, 28 Jun 2017 05:07:19: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:07:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:07:19: 6000000 INFO @ Wed, 28 Jun 2017 05:07:19: 6000000 INFO @ Wed, 28 Jun 2017 05:07:19: #1 tags after filtering in treatment: 2691184 INFO @ Wed, 28 Jun 2017 05:07:19: #1 Redundant rate of treatment: 0.12 INFO @ Wed, 28 Jun 2017 05:07:19: #1 finished! INFO @ Wed, 28 Jun 2017 05:07:19: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:07:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:07:19: #2 number of paired peaks: 31 WARNING @ Wed, 28 Jun 2017 05:07:19: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:07:19: Process for pairing-model is terminated! cat: SRX1419781.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1419781.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1419781.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1419781.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 05:07:21: #1 tag size is determined as 94 bps INFO @ Wed, 28 Jun 2017 05:07:21: #1 tag size = 94 INFO @ Wed, 28 Jun 2017 05:07:21: #1 total tags in treatment: 3066504 INFO @ Wed, 28 Jun 2017 05:07:21: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:07:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:07:21: #1 tags after filtering in treatment: 2691184 INFO @ Wed, 28 Jun 2017 05:07:21: #1 Redundant rate of treatment: 0.12 INFO @ Wed, 28 Jun 2017 05:07:21: #1 finished! INFO @ Wed, 28 Jun 2017 05:07:21: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:07:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:07:21: #1 tag size is determined as 94 bps INFO @ Wed, 28 Jun 2017 05:07:21: #1 tag size = 94 INFO @ Wed, 28 Jun 2017 05:07:21: #1 total tags in treatment: 3066504 INFO @ Wed, 28 Jun 2017 05:07:21: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:07:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:07:21: #1 tags after filtering in treatment: 2691184 INFO @ Wed, 28 Jun 2017 05:07:21: #1 Redundant rate of treatment: 0.12 INFO @ Wed, 28 Jun 2017 05:07:21: #1 finished! INFO @ Wed, 28 Jun 2017 05:07:21: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:07:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:07:21: #2 number of paired peaks: 31 WARNING @ Wed, 28 Jun 2017 05:07:21: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:07:21: Process for pairing-model is terminated! cat: SRX1419781.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1419781.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1419781.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1419781.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 05:07:21: #2 number of paired peaks: 31 WARNING @ Wed, 28 Jun 2017 05:07:21: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:07:21: Process for pairing-model is terminated! cat: SRX1419781.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1419781.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1419781.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1419781.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。