Job ID = 2009735 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,061,436 reads read : 3,061,436 reads written : 3,061,436 spots read : 3,073,658 reads read : 3,073,658 reads written : 3,073,658 spots read : 3,066,760 reads read : 3,066,760 reads written : 3,066,760 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR441512.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR441513.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:35 9201854 reads; of these: 9201854 (100.00%) were unpaired; of these: 612451 (6.66%) aligned 0 times 7381100 (80.21%) aligned exactly 1 time 1208303 (13.13%) aligned >1 times 93.34% overall alignment rate Time searching: 00:01:35 Overall time: 00:01:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2534912 / 8589403 = 0.2951 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 05 Jul 2019 19:47:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:47:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:47:30: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:47:30: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:47:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:47:30: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:47:30: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:47:30: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:47:30: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:47:39: 1000000 INFO @ Fri, 05 Jul 2019 19:47:40: 1000000 INFO @ Fri, 05 Jul 2019 19:47:40: 1000000 INFO @ Fri, 05 Jul 2019 19:47:51: 2000000 INFO @ Fri, 05 Jul 2019 19:47:51: 2000000 INFO @ Fri, 05 Jul 2019 19:47:52: 2000000 INFO @ Fri, 05 Jul 2019 19:47:59: 3000000 INFO @ Fri, 05 Jul 2019 19:48:05: 3000000 INFO @ Fri, 05 Jul 2019 19:48:07: 3000000 INFO @ Fri, 05 Jul 2019 19:48:08: 4000000 INFO @ Fri, 05 Jul 2019 19:48:15: 4000000 INFO @ Fri, 05 Jul 2019 19:48:17: 4000000 INFO @ Fri, 05 Jul 2019 19:48:17: 5000000 INFO @ Fri, 05 Jul 2019 19:48:24: 5000000 INFO @ Fri, 05 Jul 2019 19:48:26: 6000000 INFO @ Fri, 05 Jul 2019 19:48:26: 5000000 INFO @ Fri, 05 Jul 2019 19:48:26: #1 tag size is determined as 45 bps INFO @ Fri, 05 Jul 2019 19:48:29: #1 tag size = 45 INFO @ Fri, 05 Jul 2019 19:48:29: #1 total tags in treatment: 6054491 INFO @ Fri, 05 Jul 2019 19:48:29: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:48:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:48:29: #1 tags after filtering in treatment: 6054491 INFO @ Fri, 05 Jul 2019 19:48:31: 6000000 INFO @ Fri, 05 Jul 2019 19:48:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:48:32: #1 finished! INFO @ Fri, 05 Jul 2019 19:48:32: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:48:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:48:33: #1 tag size is determined as 45 bps INFO @ Fri, 05 Jul 2019 19:48:33: #2 number of paired peaks: 0 INFO @ Fri, 05 Jul 2019 19:48:34: #1 tag size = 45 WARNING @ Fri, 05 Jul 2019 19:48:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. INFO @ Fri, 05 Jul 2019 19:48:36: #1 total tags in treatment: 6054491 WARNING @ Fri, 05 Jul 2019 19:48:36: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:48:36: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:48:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cut: /home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.10_peaks.narrowPeakINFO @ Fri, 05 Jul 2019 19:48:36: #1 tags after filtering in treatment: 6054491 : No such file or directory INFO @ Fri, 05 Jul 2019 19:48:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:48:36: #1 finished! INFO @ Fri, 05 Jul 2019 19:48:36: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:48:36: #2 looking for paired plus/minus strand peaks... pass1 - making usageList (0 chroms)INFO @ Fri, 05 Jul 2019 19:48:37: #2 number of paired peaks: 0 : 1 millis INFO @ Fri, 05 Jul 2019 19:48:37: 6000000 WARNING @ Fri, 05 Jul 2019 19:48:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:48:38: Process for pairing-model is terminated! needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.10_*.xls’cut: : No such file or directory/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 19:48:38: #1 tag size is determined as 45 bps : No such file or directory INFO @ Fri, 05 Jul 2019 19:48:41: #1 tag size = 45 INFO @ Fri, 05 Jul 2019 19:48:41: #1 total tags in treatment: 6054491 INFO @ Fri, 05 Jul 2019 19:48:41: #1 user defined the maximum tags... rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.10_peaks.narrowPeak’: No such file or directory INFO @ Fri, 05 Jul 2019 19:48:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) CompletedMACS2peakCalling pass1 - making usageList (0 chroms)INFO @ Fri, 05 Jul 2019 19:48:41: #1 tags after filtering in treatment: 6054491 : 2 millis INFO @ Fri, 05 Jul 2019 19:48:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:48:42: #1 finished! INFO @ Fri, 05 Jul 2019 19:48:42: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:48:42: #2 looking for paired plus/minus strand peaks... needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.20_model.r’INFO @ Fri, 05 Jul 2019 19:48:42: #2 number of paired peaks: 0 : No such file or directory WARNING @ Fri, 05 Jul 2019 19:48:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:48:44: Process for pairing-model is terminated! rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling cut: /home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127919/SRX127919.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。