Job ID = 2009732 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 2,680,092 reads read : 2,680,092 reads written : 2,680,092 spots read : 2,696,818 reads read : 2,696,818 reads written : 2,696,818 spots read : 2,679,768 reads read : 2,679,768 reads written : 2,679,768 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR441510.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR441511.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:08 8056678 reads; of these: 8056678 (100.00%) were unpaired; of these: 839420 (10.42%) aligned 0 times 6508538 (80.78%) aligned exactly 1 time 708720 (8.80%) aligned >1 times 89.58% overall alignment rate Time searching: 00:02:08 Overall time: 00:02:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2256616 / 7217258 = 0.3127 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:48:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:48:20: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:48:20: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:48:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:48:21: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:48:21: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:48:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:48:22: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:48:22: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:48:28: 1000000 INFO @ Fri, 05 Jul 2019 19:48:30: 1000000 INFO @ Fri, 05 Jul 2019 19:48:32: 1000000 INFO @ Fri, 05 Jul 2019 19:48:36: 2000000 INFO @ Fri, 05 Jul 2019 19:48:39: 2000000 INFO @ Fri, 05 Jul 2019 19:48:43: 2000000 INFO @ Fri, 05 Jul 2019 19:48:44: 3000000 INFO @ Fri, 05 Jul 2019 19:48:47: 3000000 INFO @ Fri, 05 Jul 2019 19:48:51: 4000000 INFO @ Fri, 05 Jul 2019 19:48:53: 3000000 INFO @ Fri, 05 Jul 2019 19:48:55: 4000000 INFO @ Fri, 05 Jul 2019 19:48:58: #1 tag size is determined as 45 bps INFO @ Fri, 05 Jul 2019 19:48:58: #1 tag size = 45 INFO @ Fri, 05 Jul 2019 19:48:58: #1 total tags in treatment: 4960642 INFO @ Fri, 05 Jul 2019 19:48:58: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:48:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:48:59: #1 tags after filtering in treatment: 4960642 INFO @ Fri, 05 Jul 2019 19:48:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:48:59: #1 finished! INFO @ Fri, 05 Jul 2019 19:48:59: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:48:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:48:59: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:48:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:48:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:49:03: 4000000 INFO @ Fri, 05 Jul 2019 19:49:03: #1 tag size is determined as 45 bps INFO @ Fri, 05 Jul 2019 19:49:03: #1 tag size = 45 INFO @ Fri, 05 Jul 2019 19:49:03: #1 total tags in treatment: 4960642 INFO @ Fri, 05 Jul 2019 19:49:03: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:49:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:49:03: #1 tags after filtering in treatment: 4960642 INFO @ Fri, 05 Jul 2019 19:49:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:49:03: #1 finished! INFO @ Fri, 05 Jul 2019 19:49:03: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:49:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:49:04: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:49:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:49:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:49:12: #1 tag size is determined as 45 bps INFO @ Fri, 05 Jul 2019 19:49:12: #1 tag size = 45 INFO @ Fri, 05 Jul 2019 19:49:12: #1 total tags in treatment: 4960642 INFO @ Fri, 05 Jul 2019 19:49:12: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:49:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:49:12: #1 tags after filtering in treatment: 4960642 INFO @ Fri, 05 Jul 2019 19:49:12: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:49:12: #1 finished! INFO @ Fri, 05 Jul 2019 19:49:12: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:49:12: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:49:12: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:49:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:49:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127918/SRX127918.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。