Job ID = 2009729 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 2,623,968 reads read : 2,623,968 reads written : 2,623,968 spots read : 2,638,874 reads read : 2,638,874 reads written : 2,638,874 spots read : 2,636,197 reads read : 2,636,197 reads written : 2,636,197 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR441504.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR441505.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:19 7899039 reads; of these: 7899039 (100.00%) were unpaired; of these: 392005 (4.96%) aligned 0 times 6390713 (80.90%) aligned exactly 1 time 1116321 (14.13%) aligned >1 times 95.04% overall alignment rate Time searching: 00:01:19 Overall time: 00:01:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2611385 / 7507034 = 0.3479 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:48:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:48:23: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:48:23: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:48:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:48:24: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:48:24: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:48:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:48:25: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:48:25: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:48:30: 1000000 INFO @ Fri, 05 Jul 2019 19:48:32: 1000000 INFO @ Fri, 05 Jul 2019 19:48:33: 1000000 INFO @ Fri, 05 Jul 2019 19:48:36: 2000000 INFO @ Fri, 05 Jul 2019 19:48:40: 2000000 INFO @ Fri, 05 Jul 2019 19:48:41: 2000000 INFO @ Fri, 05 Jul 2019 19:48:43: 3000000 INFO @ Fri, 05 Jul 2019 19:48:48: 3000000 INFO @ Fri, 05 Jul 2019 19:48:48: 3000000 INFO @ Fri, 05 Jul 2019 19:48:49: 4000000 INFO @ Fri, 05 Jul 2019 19:48:55: #1 tag size is determined as 45 bps INFO @ Fri, 05 Jul 2019 19:48:55: #1 tag size = 45 INFO @ Fri, 05 Jul 2019 19:48:55: #1 total tags in treatment: 4895649 INFO @ Fri, 05 Jul 2019 19:48:55: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:48:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:48:55: #1 tags after filtering in treatment: 4895649 INFO @ Fri, 05 Jul 2019 19:48:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:48:55: #1 finished! INFO @ Fri, 05 Jul 2019 19:48:55: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:48:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:48:55: 4000000 INFO @ Fri, 05 Jul 2019 19:48:55: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:48:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:48:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:48:56: 4000000 INFO @ Fri, 05 Jul 2019 19:49:01: #1 tag size is determined as 45 bps INFO @ Fri, 05 Jul 2019 19:49:01: #1 tag size = 45 INFO @ Fri, 05 Jul 2019 19:49:01: #1 total tags in treatment: 4895649 INFO @ Fri, 05 Jul 2019 19:49:01: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:49:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:49:02: #1 tags after filtering in treatment: 4895649 INFO @ Fri, 05 Jul 2019 19:49:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:49:02: #1 finished! INFO @ Fri, 05 Jul 2019 19:49:02: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:49:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:49:02: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:49:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:49:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:49:03: #1 tag size is determined as 45 bps INFO @ Fri, 05 Jul 2019 19:49:03: #1 tag size = 45 INFO @ Fri, 05 Jul 2019 19:49:03: #1 total tags in treatment: 4895649 INFO @ Fri, 05 Jul 2019 19:49:03: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:49:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:49:03: #1 tags after filtering in treatment: 4895649 INFO @ Fri, 05 Jul 2019 19:49:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:49:03: #1 finished! INFO @ Fri, 05 Jul 2019 19:49:03: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:49:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:49:03: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:49:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:49:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX127916/SRX127916.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。