Job ID = 9161892 sra ファイルのダウンロード中... Completed: 762474K bytes transferred in 8 seconds (759467K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 38221421 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1241204/SRR2394719.sra Written 38221421 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:51 38221421 reads; of these: 38221421 (100.00%) were unpaired; of these: 1578116 (4.13%) aligned 0 times 31595314 (82.66%) aligned exactly 1 time 5047991 (13.21%) aligned >1 times 95.87% overall alignment rate Time searching: 00:06:51 Overall time: 00:06:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18995989 / 36643305 = 0.5184 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 05:07:38: # Command line: callpeak -t SRX1241204.bam -f BAM -g 12100000 -n SRX1241204.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1241204.20 # format = BAM # ChIP-seq file = ['SRX1241204.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:07:38: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:07:38: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:07:38: # Command line: callpeak -t SRX1241204.bam -f BAM -g 12100000 -n SRX1241204.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1241204.05 # format = BAM # ChIP-seq file = ['SRX1241204.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:07:38: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:07:38: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:07:38: # Command line: callpeak -t SRX1241204.bam -f BAM -g 12100000 -n SRX1241204.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1241204.10 # format = BAM # ChIP-seq file = ['SRX1241204.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:07:38: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:07:38: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:07:45: 1000000 INFO @ Wed, 28 Jun 2017 05:07:45: 1000000 INFO @ Wed, 28 Jun 2017 05:07:45: 1000000 INFO @ Wed, 28 Jun 2017 05:07:51: 2000000 INFO @ Wed, 28 Jun 2017 05:07:52: 2000000 INFO @ Wed, 28 Jun 2017 05:07:52: 2000000 INFO @ Wed, 28 Jun 2017 05:07:57: 3000000 INFO @ Wed, 28 Jun 2017 05:07:59: 3000000 INFO @ Wed, 28 Jun 2017 05:07:59: 3000000 INFO @ Wed, 28 Jun 2017 05:08:03: 4000000 INFO @ Wed, 28 Jun 2017 05:08:06: 4000000 INFO @ Wed, 28 Jun 2017 05:08:06: 4000000 INFO @ Wed, 28 Jun 2017 05:08:09: 5000000 INFO @ Wed, 28 Jun 2017 05:08:14: 5000000 INFO @ Wed, 28 Jun 2017 05:08:14: 5000000 INFO @ Wed, 28 Jun 2017 05:08:16: 6000000 INFO @ Wed, 28 Jun 2017 05:08:21: 6000000 INFO @ Wed, 28 Jun 2017 05:08:21: 6000000 INFO @ Wed, 28 Jun 2017 05:08:22: 7000000 INFO @ Wed, 28 Jun 2017 05:08:28: 7000000 INFO @ Wed, 28 Jun 2017 05:08:28: 7000000 INFO @ Wed, 28 Jun 2017 05:08:29: 8000000 INFO @ Wed, 28 Jun 2017 05:08:35: 8000000 INFO @ Wed, 28 Jun 2017 05:08:35: 8000000 INFO @ Wed, 28 Jun 2017 05:08:35: 9000000 INFO @ Wed, 28 Jun 2017 05:08:42: 10000000 INFO @ Wed, 28 Jun 2017 05:08:42: 9000000 INFO @ Wed, 28 Jun 2017 05:08:42: 9000000 INFO @ Wed, 28 Jun 2017 05:08:48: 11000000 INFO @ Wed, 28 Jun 2017 05:08:49: 10000000 INFO @ Wed, 28 Jun 2017 05:08:49: 10000000 INFO @ Wed, 28 Jun 2017 05:08:54: 12000000 INFO @ Wed, 28 Jun 2017 05:08:57: 11000000 INFO @ Wed, 28 Jun 2017 05:08:57: 11000000 INFO @ Wed, 28 Jun 2017 05:09:01: 13000000 INFO @ Wed, 28 Jun 2017 05:09:04: 12000000 INFO @ Wed, 28 Jun 2017 05:09:04: 12000000 INFO @ Wed, 28 Jun 2017 05:09:07: 14000000 INFO @ Wed, 28 Jun 2017 05:09:12: 13000000 INFO @ Wed, 28 Jun 2017 05:09:12: 13000000 INFO @ Wed, 28 Jun 2017 05:09:14: 15000000 INFO @ Wed, 28 Jun 2017 05:09:19: 14000000 INFO @ Wed, 28 Jun 2017 05:09:19: 14000000 INFO @ Wed, 28 Jun 2017 05:09:20: 16000000 INFO @ Wed, 28 Jun 2017 05:09:26: 15000000 INFO @ Wed, 28 Jun 2017 05:09:26: 15000000 INFO @ Wed, 28 Jun 2017 05:09:26: 17000000 INFO @ Wed, 28 Jun 2017 05:09:31: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:09:31: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:09:31: #1 total tags in treatment: 17647316 INFO @ Wed, 28 Jun 2017 05:09:31: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:09:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:09:31: #1 tags after filtering in treatment: 17647316 INFO @ Wed, 28 Jun 2017 05:09:31: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:09:31: #1 finished! INFO @ Wed, 28 Jun 2017 05:09:31: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:09:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:09:32: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:09:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:09:32: Process for pairing-model is terminated! cat: SRX1241204.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241204.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241204.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241204.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 05:09:33: 16000000 INFO @ Wed, 28 Jun 2017 05:09:33: 16000000 INFO @ Wed, 28 Jun 2017 05:09:40: 17000000 INFO @ Wed, 28 Jun 2017 05:09:40: 17000000 INFO @ Wed, 28 Jun 2017 05:09:45: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:09:45: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:09:45: #1 total tags in treatment: 17647316 INFO @ Wed, 28 Jun 2017 05:09:45: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:09:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:09:45: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:09:45: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:09:45: #1 total tags in treatment: 17647316 INFO @ Wed, 28 Jun 2017 05:09:45: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:09:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:09:45: #1 tags after filtering in treatment: 17647316 INFO @ Wed, 28 Jun 2017 05:09:45: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:09:45: #1 finished! INFO @ Wed, 28 Jun 2017 05:09:45: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:09:45: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:09:45: #1 tags after filtering in treatment: 17647316 INFO @ Wed, 28 Jun 2017 05:09:45: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:09:45: #1 finished! INFO @ Wed, 28 Jun 2017 05:09:45: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:09:45: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:09:46: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:09:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:09:46: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 05:09:46: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:09:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:09:46: Process for pairing-model is terminated! cat: SRX1241204.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX1241204.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis rm: cannot remove `SRX1241204.20_model.r'needLargeMem: trying to allocate 0 bytes (limit: 17179869184) : そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241204.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241204.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling rm: cannot remove `SRX1241204.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241204.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241204.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。