Job ID = 9161890 sra ファイルのダウンロード中... Completed: 557830K bytes transferred in 7 seconds (622290K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 27664468 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1241202/SRR2394717.sra Written 27664468 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:24 27664468 reads; of these: 27664468 (100.00%) were unpaired; of these: 1019669 (3.69%) aligned 0 times 23257270 (84.07%) aligned exactly 1 time 3387529 (12.25%) aligned >1 times 96.31% overall alignment rate Time searching: 00:05:24 Overall time: 00:05:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11566253 / 26644799 = 0.4341 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 05:03:58: # Command line: callpeak -t SRX1241202.bam -f BAM -g 12100000 -n SRX1241202.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1241202.10 # format = BAM # ChIP-seq file = ['SRX1241202.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:03:58: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:03:58: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:03:58: # Command line: callpeak -t SRX1241202.bam -f BAM -g 12100000 -n SRX1241202.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1241202.05 # format = BAM # ChIP-seq file = ['SRX1241202.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:03:58: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:03:58: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:03:58: # Command line: callpeak -t SRX1241202.bam -f BAM -g 12100000 -n SRX1241202.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1241202.20 # format = BAM # ChIP-seq file = ['SRX1241202.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:03:58: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:03:58: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:04:06: 1000000 INFO @ Wed, 28 Jun 2017 05:04:06: 1000000 INFO @ Wed, 28 Jun 2017 05:04:06: 1000000 INFO @ Wed, 28 Jun 2017 05:04:13: 2000000 INFO @ Wed, 28 Jun 2017 05:04:13: 2000000 INFO @ Wed, 28 Jun 2017 05:04:13: 2000000 INFO @ Wed, 28 Jun 2017 05:04:21: 3000000 INFO @ Wed, 28 Jun 2017 05:04:21: 3000000 INFO @ Wed, 28 Jun 2017 05:04:21: 3000000 INFO @ Wed, 28 Jun 2017 05:04:28: 4000000 INFO @ Wed, 28 Jun 2017 05:04:29: 4000000 INFO @ Wed, 28 Jun 2017 05:04:29: 4000000 INFO @ Wed, 28 Jun 2017 05:04:36: 5000000 INFO @ Wed, 28 Jun 2017 05:04:36: 5000000 INFO @ Wed, 28 Jun 2017 05:04:36: 5000000 INFO @ Wed, 28 Jun 2017 05:04:43: 6000000 INFO @ Wed, 28 Jun 2017 05:04:44: 6000000 INFO @ Wed, 28 Jun 2017 05:04:44: 6000000 INFO @ Wed, 28 Jun 2017 05:04:51: 7000000 INFO @ Wed, 28 Jun 2017 05:04:51: 7000000 INFO @ Wed, 28 Jun 2017 05:04:51: 7000000 INFO @ Wed, 28 Jun 2017 05:04:59: 8000000 INFO @ Wed, 28 Jun 2017 05:04:59: 8000000 INFO @ Wed, 28 Jun 2017 05:04:59: 8000000 INFO @ Wed, 28 Jun 2017 05:05:07: 9000000 INFO @ Wed, 28 Jun 2017 05:05:07: 9000000 INFO @ Wed, 28 Jun 2017 05:05:07: 9000000 INFO @ Wed, 28 Jun 2017 05:05:15: 10000000 INFO @ Wed, 28 Jun 2017 05:05:15: 10000000 INFO @ Wed, 28 Jun 2017 05:05:15: 10000000 INFO @ Wed, 28 Jun 2017 05:05:23: 11000000 INFO @ Wed, 28 Jun 2017 05:05:23: 11000000 INFO @ Wed, 28 Jun 2017 05:05:23: 11000000 INFO @ Wed, 28 Jun 2017 05:05:31: 12000000 INFO @ Wed, 28 Jun 2017 05:05:31: 12000000 INFO @ Wed, 28 Jun 2017 05:05:31: 12000000 INFO @ Wed, 28 Jun 2017 05:05:38: 13000000 INFO @ Wed, 28 Jun 2017 05:05:39: 13000000 INFO @ Wed, 28 Jun 2017 05:05:39: 13000000 INFO @ Wed, 28 Jun 2017 05:05:46: 14000000 INFO @ Wed, 28 Jun 2017 05:05:48: 14000000 INFO @ Wed, 28 Jun 2017 05:05:48: 14000000 INFO @ Wed, 28 Jun 2017 05:05:54: 15000000 INFO @ Wed, 28 Jun 2017 05:05:54: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:05:54: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:05:54: #1 total tags in treatment: 15078546 INFO @ Wed, 28 Jun 2017 05:05:54: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:05:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:05:55: #1 tags after filtering in treatment: 15078546 INFO @ Wed, 28 Jun 2017 05:05:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:05:55: #1 finished! INFO @ Wed, 28 Jun 2017 05:05:55: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:05:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:05:55: 15000000 INFO @ Wed, 28 Jun 2017 05:05:55: 15000000 INFO @ Wed, 28 Jun 2017 05:05:56: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:05:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:05:56: Process for pairing-model is terminated! cat: SRX1241202.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241202.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241202.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241202.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 05:05:56: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:05:56: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:05:56: #1 total tags in treatment: 15078546 INFO @ Wed, 28 Jun 2017 05:05:56: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:05:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:05:56: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:05:56: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:05:56: #1 total tags in treatment: 15078546 INFO @ Wed, 28 Jun 2017 05:05:56: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:05:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:05:56: #1 tags after filtering in treatment: 15078546 INFO @ Wed, 28 Jun 2017 05:05:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:05:56: #1 finished! INFO @ Wed, 28 Jun 2017 05:05:56: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:05:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:05:56: #1 tags after filtering in treatment: 15078546 INFO @ Wed, 28 Jun 2017 05:05:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:05:56: #1 finished! INFO @ Wed, 28 Jun 2017 05:05:56: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:05:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:05:57: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:05:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:05:57: Process for pairing-model is terminated! cat: SRX1241202.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241202.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241202.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241202.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 05:05:57: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:05:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:05:57: Process for pairing-model is terminated! cat: SRX1241202.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241202.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241202.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241202.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。