Job ID = 9161888 sra ファイルのダウンロード中... Completed: 597139K bytes transferred in 7 seconds (693350K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 29882465 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1241200/SRR2394715.sra Written 29882465 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:28 29882465 reads; of these: 29882465 (100.00%) were unpaired; of these: 1016775 (3.40%) aligned 0 times 25146649 (84.15%) aligned exactly 1 time 3719041 (12.45%) aligned >1 times 96.60% overall alignment rate Time searching: 00:05:28 Overall time: 00:05:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13091964 / 28865690 = 0.4535 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 05:04:16: # Command line: callpeak -t SRX1241200.bam -f BAM -g 12100000 -n SRX1241200.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1241200.10 # format = BAM # ChIP-seq file = ['SRX1241200.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:04:16: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:04:16: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:04:16: # Command line: callpeak -t SRX1241200.bam -f BAM -g 12100000 -n SRX1241200.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1241200.20 # format = BAM # ChIP-seq file = ['SRX1241200.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:04:16: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:04:16: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:04:16: # Command line: callpeak -t SRX1241200.bam -f BAM -g 12100000 -n SRX1241200.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1241200.05 # format = BAM # ChIP-seq file = ['SRX1241200.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:04:16: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:04:16: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:04:22: 1000000 INFO @ Wed, 28 Jun 2017 05:04:23: 1000000 INFO @ Wed, 28 Jun 2017 05:04:23: 1000000 INFO @ Wed, 28 Jun 2017 05:04:29: 2000000 INFO @ Wed, 28 Jun 2017 05:04:30: 2000000 INFO @ Wed, 28 Jun 2017 05:04:30: 2000000 INFO @ Wed, 28 Jun 2017 05:04:35: 3000000 INFO @ Wed, 28 Jun 2017 05:04:38: 3000000 INFO @ Wed, 28 Jun 2017 05:04:38: 3000000 INFO @ Wed, 28 Jun 2017 05:04:41: 4000000 INFO @ Wed, 28 Jun 2017 05:04:45: 4000000 INFO @ Wed, 28 Jun 2017 05:04:45: 4000000 INFO @ Wed, 28 Jun 2017 05:04:48: 5000000 INFO @ Wed, 28 Jun 2017 05:04:52: 5000000 INFO @ Wed, 28 Jun 2017 05:04:52: 5000000 INFO @ Wed, 28 Jun 2017 05:04:55: 6000000 INFO @ Wed, 28 Jun 2017 05:05:00: 6000000 INFO @ Wed, 28 Jun 2017 05:05:00: 6000000 INFO @ Wed, 28 Jun 2017 05:05:01: 7000000 INFO @ Wed, 28 Jun 2017 05:05:07: 7000000 INFO @ Wed, 28 Jun 2017 05:05:07: 7000000 INFO @ Wed, 28 Jun 2017 05:05:07: 8000000 INFO @ Wed, 28 Jun 2017 05:05:13: 9000000 INFO @ Wed, 28 Jun 2017 05:05:13: 8000000 INFO @ Wed, 28 Jun 2017 05:05:13: 8000000 INFO @ Wed, 28 Jun 2017 05:05:19: 9000000 INFO @ Wed, 28 Jun 2017 05:05:19: 9000000 INFO @ Wed, 28 Jun 2017 05:05:20: 10000000 INFO @ Wed, 28 Jun 2017 05:05:25: 10000000 INFO @ Wed, 28 Jun 2017 05:05:25: 10000000 INFO @ Wed, 28 Jun 2017 05:05:26: 11000000 INFO @ Wed, 28 Jun 2017 05:05:31: 11000000 INFO @ Wed, 28 Jun 2017 05:05:32: 11000000 INFO @ Wed, 28 Jun 2017 05:05:33: 12000000 INFO @ Wed, 28 Jun 2017 05:05:38: 12000000 INFO @ Wed, 28 Jun 2017 05:05:38: 12000000 INFO @ Wed, 28 Jun 2017 05:05:40: 13000000 INFO @ Wed, 28 Jun 2017 05:05:44: 13000000 INFO @ Wed, 28 Jun 2017 05:05:45: 13000000 INFO @ Wed, 28 Jun 2017 05:05:48: 14000000 INFO @ Wed, 28 Jun 2017 05:05:52: 14000000 INFO @ Wed, 28 Jun 2017 05:05:52: 14000000 INFO @ Wed, 28 Jun 2017 05:05:56: 15000000 INFO @ Wed, 28 Jun 2017 05:05:59: 15000000 INFO @ Wed, 28 Jun 2017 05:05:59: 15000000 INFO @ Wed, 28 Jun 2017 05:06:02: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:06:02: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:06:02: #1 total tags in treatment: 15773726 INFO @ Wed, 28 Jun 2017 05:06:02: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:06:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:06:02: #1 tags after filtering in treatment: 15773726 INFO @ Wed, 28 Jun 2017 05:06:02: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:06:02: #1 finished! INFO @ Wed, 28 Jun 2017 05:06:02: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:06:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:06:03: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:06:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:06:03: Process for pairing-model is terminated! cat: SRX1241200.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241200.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241200.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241200.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 05:06:04: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:06:04: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:06:04: #1 total tags in treatment: 15773726 INFO @ Wed, 28 Jun 2017 05:06:04: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:06:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:06:04: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:06:04: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:06:04: #1 total tags in treatment: 15773726 INFO @ Wed, 28 Jun 2017 05:06:04: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:06:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:06:04: #1 tags after filtering in treatment: 15773726 INFO @ Wed, 28 Jun 2017 05:06:04: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:06:04: #1 finished! INFO @ Wed, 28 Jun 2017 05:06:04: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:06:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:06:05: #1 tags after filtering in treatment: 15773726 INFO @ Wed, 28 Jun 2017 05:06:05: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:06:05: #1 finished! INFO @ Wed, 28 Jun 2017 05:06:05: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:06:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:06:05: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:06:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:06:05: Process for pairing-model is terminated! cat: SRX1241200.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241200.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241200.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241200.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 05:06:06: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:06:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:06:06: Process for pairing-model is terminated! cat: SRX1241200.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241200.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241200.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241200.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。