Job ID = 9161879 sra ファイルのダウンロード中... Completed: 306189K bytes transferred in 5 seconds (477285K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 15508486 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1241191/SRR2394706.sra Written 15508486 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:52 15508486 reads; of these: 15508486 (100.00%) were unpaired; of these: 685803 (4.42%) aligned 0 times 12366389 (79.74%) aligned exactly 1 time 2456294 (15.84%) aligned >1 times 95.58% overall alignment rate Time searching: 00:02:52 Overall time: 00:02:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4903183 / 14822683 = 0.3308 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:57:30: # Command line: callpeak -t SRX1241191.bam -f BAM -g 12100000 -n SRX1241191.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1241191.10 # format = BAM # ChIP-seq file = ['SRX1241191.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:57:30: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:57:30: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:57:30: # Command line: callpeak -t SRX1241191.bam -f BAM -g 12100000 -n SRX1241191.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1241191.05 # format = BAM # ChIP-seq file = ['SRX1241191.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:57:30: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:57:30: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:57:30: # Command line: callpeak -t SRX1241191.bam -f BAM -g 12100000 -n SRX1241191.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1241191.20 # format = BAM # ChIP-seq file = ['SRX1241191.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:57:30: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:57:30: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:57:37: 1000000 INFO @ Wed, 28 Jun 2017 04:57:37: 1000000 INFO @ Wed, 28 Jun 2017 04:57:37: 1000000 INFO @ Wed, 28 Jun 2017 04:57:44: 2000000 INFO @ Wed, 28 Jun 2017 04:57:44: 2000000 INFO @ Wed, 28 Jun 2017 04:57:44: 2000000 INFO @ Wed, 28 Jun 2017 04:57:50: 3000000 INFO @ Wed, 28 Jun 2017 04:57:51: 3000000 INFO @ Wed, 28 Jun 2017 04:57:51: 3000000 INFO @ Wed, 28 Jun 2017 04:57:56: 4000000 INFO @ Wed, 28 Jun 2017 04:57:57: 4000000 INFO @ Wed, 28 Jun 2017 04:57:57: 4000000 INFO @ Wed, 28 Jun 2017 04:58:02: 5000000 INFO @ Wed, 28 Jun 2017 04:58:03: 5000000 INFO @ Wed, 28 Jun 2017 04:58:03: 5000000 INFO @ Wed, 28 Jun 2017 04:58:09: 6000000 INFO @ Wed, 28 Jun 2017 04:58:10: 6000000 INFO @ Wed, 28 Jun 2017 04:58:10: 6000000 INFO @ Wed, 28 Jun 2017 04:58:15: 7000000 INFO @ Wed, 28 Jun 2017 04:58:16: 7000000 INFO @ Wed, 28 Jun 2017 04:58:16: 7000000 INFO @ Wed, 28 Jun 2017 04:58:21: 8000000 INFO @ Wed, 28 Jun 2017 04:58:22: 8000000 INFO @ Wed, 28 Jun 2017 04:58:23: 8000000 INFO @ Wed, 28 Jun 2017 04:58:27: 9000000 INFO @ Wed, 28 Jun 2017 04:58:29: 9000000 INFO @ Wed, 28 Jun 2017 04:58:29: 9000000 INFO @ Wed, 28 Jun 2017 04:58:33: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:58:33: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:58:33: #1 total tags in treatment: 9919500 INFO @ Wed, 28 Jun 2017 04:58:33: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:58:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:58:33: #1 tags after filtering in treatment: 9919500 INFO @ Wed, 28 Jun 2017 04:58:33: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:58:33: #1 finished! INFO @ Wed, 28 Jun 2017 04:58:33: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:58:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:58:34: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:58:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:58:34: Process for pairing-model is terminated! cat: SRX1241191.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241191.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241191.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241191.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:58:34: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:58:34: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:58:34: #1 total tags in treatment: 9919500 INFO @ Wed, 28 Jun 2017 04:58:34: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:58:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:58:35: #1 tags after filtering in treatment: 9919500 INFO @ Wed, 28 Jun 2017 04:58:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:58:35: #1 finished! INFO @ Wed, 28 Jun 2017 04:58:35: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:58:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:58:35: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:58:35: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:58:35: #1 total tags in treatment: 9919500 INFO @ Wed, 28 Jun 2017 04:58:35: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:58:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:58:35: #1 tags after filtering in treatment: 9919500 INFO @ Wed, 28 Jun 2017 04:58:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:58:35: #1 finished! INFO @ Wed, 28 Jun 2017 04:58:35: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:58:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:58:35: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:58:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:58:35: Process for pairing-model is terminated! cat: SRX1241191.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241191.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241191.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241191.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:58:36: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:58:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:58:36: Process for pairing-model is terminated! cat: SRX1241191.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241191.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241191.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241191.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。