Job ID = 9161878 sra ファイルのダウンロード中... Completed: 626497K bytes transferred in 8 seconds (631430K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 31328516 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1241190/SRR2394705.sra Written 31328516 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:33 31328516 reads; of these: 31328516 (100.00%) were unpaired; of these: 1034022 (3.30%) aligned 0 times 26386785 (84.23%) aligned exactly 1 time 3907709 (12.47%) aligned >1 times 96.70% overall alignment rate Time searching: 00:05:33 Overall time: 00:05:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 14075646 / 30294494 = 0.4646 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 05:03:19: # Command line: callpeak -t SRX1241190.bam -f BAM -g 12100000 -n SRX1241190.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1241190.10 # format = BAM # ChIP-seq file = ['SRX1241190.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:03:19: # Command line: callpeak -t SRX1241190.bam -f BAM -g 12100000 -n SRX1241190.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1241190.05 # format = BAM # ChIP-seq file = ['SRX1241190.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:03:19: # Command line: callpeak -t SRX1241190.bam -f BAM -g 12100000 -n SRX1241190.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1241190.20 # format = BAM # ChIP-seq file = ['SRX1241190.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:03:19: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:03:19: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:03:19: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:03:19: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:03:19: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:03:19: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:03:25: 1000000 INFO @ Wed, 28 Jun 2017 05:03:25: 1000000 INFO @ Wed, 28 Jun 2017 05:03:25: 1000000 INFO @ Wed, 28 Jun 2017 05:03:31: 2000000 INFO @ Wed, 28 Jun 2017 05:03:31: 2000000 INFO @ Wed, 28 Jun 2017 05:03:31: 2000000 INFO @ Wed, 28 Jun 2017 05:03:37: 3000000 INFO @ Wed, 28 Jun 2017 05:03:37: 3000000 INFO @ Wed, 28 Jun 2017 05:03:37: 3000000 INFO @ Wed, 28 Jun 2017 05:03:43: 4000000 INFO @ Wed, 28 Jun 2017 05:03:44: 4000000 INFO @ Wed, 28 Jun 2017 05:03:44: 4000000 INFO @ Wed, 28 Jun 2017 05:03:49: 5000000 INFO @ Wed, 28 Jun 2017 05:03:50: 5000000 INFO @ Wed, 28 Jun 2017 05:03:50: 5000000 INFO @ Wed, 28 Jun 2017 05:03:54: 6000000 INFO @ Wed, 28 Jun 2017 05:03:56: 6000000 INFO @ Wed, 28 Jun 2017 05:03:56: 6000000 INFO @ Wed, 28 Jun 2017 05:04:00: 7000000 INFO @ Wed, 28 Jun 2017 05:04:02: 7000000 INFO @ Wed, 28 Jun 2017 05:04:02: 7000000 INFO @ Wed, 28 Jun 2017 05:04:06: 8000000 INFO @ Wed, 28 Jun 2017 05:04:09: 8000000 INFO @ Wed, 28 Jun 2017 05:04:09: 8000000 INFO @ Wed, 28 Jun 2017 05:04:12: 9000000 INFO @ Wed, 28 Jun 2017 05:04:15: 9000000 INFO @ Wed, 28 Jun 2017 05:04:15: 9000000 INFO @ Wed, 28 Jun 2017 05:04:19: 10000000 INFO @ Wed, 28 Jun 2017 05:04:22: 10000000 INFO @ Wed, 28 Jun 2017 05:04:22: 10000000 INFO @ Wed, 28 Jun 2017 05:04:25: 11000000 INFO @ Wed, 28 Jun 2017 05:04:28: 11000000 INFO @ Wed, 28 Jun 2017 05:04:28: 11000000 INFO @ Wed, 28 Jun 2017 05:04:30: 12000000 INFO @ Wed, 28 Jun 2017 05:04:34: 12000000 INFO @ Wed, 28 Jun 2017 05:04:34: 12000000 INFO @ Wed, 28 Jun 2017 05:04:36: 13000000 INFO @ Wed, 28 Jun 2017 05:04:40: 13000000 INFO @ Wed, 28 Jun 2017 05:04:40: 13000000 INFO @ Wed, 28 Jun 2017 05:04:42: 14000000 INFO @ Wed, 28 Jun 2017 05:04:47: 14000000 INFO @ Wed, 28 Jun 2017 05:04:47: 14000000 INFO @ Wed, 28 Jun 2017 05:04:48: 15000000 INFO @ Wed, 28 Jun 2017 05:04:53: 15000000 INFO @ Wed, 28 Jun 2017 05:04:53: 15000000 INFO @ Wed, 28 Jun 2017 05:04:54: 16000000 INFO @ Wed, 28 Jun 2017 05:04:55: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:04:55: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:04:55: #1 total tags in treatment: 16218848 INFO @ Wed, 28 Jun 2017 05:04:55: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:04:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:04:55: #1 tags after filtering in treatment: 16218848 INFO @ Wed, 28 Jun 2017 05:04:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:04:55: #1 finished! INFO @ Wed, 28 Jun 2017 05:04:55: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:04:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:04:56: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:04:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:04:56: Process for pairing-model is terminated! cat: SRX1241190.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241190.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241190.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241190.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 05:05:00: 16000000 INFO @ Wed, 28 Jun 2017 05:05:00: 16000000 INFO @ Wed, 28 Jun 2017 05:05:01: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:05:01: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:05:01: #1 total tags in treatment: 16218848 INFO @ Wed, 28 Jun 2017 05:05:01: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:05:01: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 05:05:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:05:01: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 05:05:01: #1 total tags in treatment: 16218848 INFO @ Wed, 28 Jun 2017 05:05:01: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:05:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:05:01: #1 tags after filtering in treatment: 16218848 INFO @ Wed, 28 Jun 2017 05:05:01: #1 tags after filtering in treatment: 16218848 INFO @ Wed, 28 Jun 2017 05:05:01: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:05:01: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:05:01: #1 finished! INFO @ Wed, 28 Jun 2017 05:05:01: #1 finished! INFO @ Wed, 28 Jun 2017 05:05:01: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:05:01: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:05:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:05:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:05:02: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:05:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:05:02: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 05:05:02: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:05:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:05:02: Process for pairing-model is terminated! cat: SRX1241190.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis cat: SRX1241190.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241190.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241190.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241190.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1241190.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241190.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1241190.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。