Job ID = 9161876 sra ファイルのダウンロード中... Completed: 834566K bytes transferred in 9 seconds (709868K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 29194707 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1182002/SRR2239570.sra Written 29194707 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:26 29194707 reads; of these: 29194707 (100.00%) were unpaired; of these: 197001 (0.67%) aligned 0 times 21249595 (72.79%) aligned exactly 1 time 7748111 (26.54%) aligned >1 times 99.33% overall alignment rate Time searching: 00:05:26 Overall time: 00:05:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 21575463 / 28997706 = 0.7440 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 05:02:46: # Command line: callpeak -t SRX1182002.bam -f BAM -g 12100000 -n SRX1182002.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1182002.10 # format = BAM # ChIP-seq file = ['SRX1182002.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:02:46: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:02:46: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:02:46: # Command line: callpeak -t SRX1182002.bam -f BAM -g 12100000 -n SRX1182002.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1182002.20 # format = BAM # ChIP-seq file = ['SRX1182002.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:02:46: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:02:46: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:02:47: # Command line: callpeak -t SRX1182002.bam -f BAM -g 12100000 -n SRX1182002.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1182002.05 # format = BAM # ChIP-seq file = ['SRX1182002.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 05:02:47: #1 read tag files... INFO @ Wed, 28 Jun 2017 05:02:47: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 05:02:53: 1000000 INFO @ Wed, 28 Jun 2017 05:02:54: 1000000 INFO @ Wed, 28 Jun 2017 05:02:54: 1000000 INFO @ Wed, 28 Jun 2017 05:03:00: 2000000 INFO @ Wed, 28 Jun 2017 05:03:01: 2000000 INFO @ Wed, 28 Jun 2017 05:03:01: 2000000 INFO @ Wed, 28 Jun 2017 05:03:06: 3000000 INFO @ Wed, 28 Jun 2017 05:03:09: 3000000 INFO @ Wed, 28 Jun 2017 05:03:09: 3000000 INFO @ Wed, 28 Jun 2017 05:03:13: 4000000 INFO @ Wed, 28 Jun 2017 05:03:16: 4000000 INFO @ Wed, 28 Jun 2017 05:03:16: 4000000 INFO @ Wed, 28 Jun 2017 05:03:19: 5000000 INFO @ Wed, 28 Jun 2017 05:03:24: 5000000 INFO @ Wed, 28 Jun 2017 05:03:24: 5000000 INFO @ Wed, 28 Jun 2017 05:03:26: 6000000 INFO @ Wed, 28 Jun 2017 05:03:31: 6000000 INFO @ Wed, 28 Jun 2017 05:03:31: 6000000 INFO @ Wed, 28 Jun 2017 05:03:33: 7000000 INFO @ Wed, 28 Jun 2017 05:03:36: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 05:03:36: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 05:03:36: #1 total tags in treatment: 7422243 INFO @ Wed, 28 Jun 2017 05:03:36: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:03:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:03:36: #1 tags after filtering in treatment: 7422243 INFO @ Wed, 28 Jun 2017 05:03:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:03:36: #1 finished! INFO @ Wed, 28 Jun 2017 05:03:36: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:03:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:03:36: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:03:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:03:36: Process for pairing-model is terminated! cat: SRX1182002.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1182002.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1182002.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1182002.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 05:03:38: 7000000 INFO @ Wed, 28 Jun 2017 05:03:38: 7000000 INFO @ Wed, 28 Jun 2017 05:03:40: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 05:03:40: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 05:03:40: #1 total tags in treatment: 7422243 INFO @ Wed, 28 Jun 2017 05:03:40: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:03:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:03:40: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 05:03:40: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 05:03:40: #1 total tags in treatment: 7422243 INFO @ Wed, 28 Jun 2017 05:03:40: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 05:03:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 05:03:41: #1 tags after filtering in treatment: 7422243 INFO @ Wed, 28 Jun 2017 05:03:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:03:41: #1 finished! INFO @ Wed, 28 Jun 2017 05:03:41: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:03:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:03:41: #1 tags after filtering in treatment: 7422243 INFO @ Wed, 28 Jun 2017 05:03:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 05:03:41: #1 finished! INFO @ Wed, 28 Jun 2017 05:03:41: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 05:03:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 05:03:41: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:03:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:03:41: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 05:03:41: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 05:03:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 05:03:41: Process for pairing-model is terminated! cat: SRX1182002.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis cat: SRX1182002.05_peaks.narrowPeakneedLargeMem: trying to allocate 0 bytes (limit: 17179869184) : そのようなファイルやディレクトリはありません rm: cannot remove `SRX1182002.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1182002.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1182002.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1182002.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1182002.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1182002.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。