Job ID = 14520466 SRX = SRX11781156 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:10:06 prefetch.2.10.7: 1) Downloading 'SRR15481099'... 2022-01-15T10:10:06 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:10:22 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:10:22 prefetch.2.10.7: 'SRR15481099' is valid 2022-01-15T10:10:22 prefetch.2.10.7: 1) 'SRR15481099' was downloaded successfully 2022-01-15T10:10:22 prefetch.2.10.7: 'SRR15481099' has 0 unresolved dependencies Read 7757533 spots for SRR15481099/SRR15481099.sra Written 7757533 spots for SRR15481099/SRR15481099.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:15 7757533 reads; of these: 7757533 (100.00%) were paired; of these: 4725644 (60.92%) aligned concordantly 0 times 2477936 (31.94%) aligned concordantly exactly 1 time 553953 (7.14%) aligned concordantly >1 times ---- 4725644 pairs aligned concordantly 0 times; of these: 20360 (0.43%) aligned discordantly 1 time ---- 4705284 pairs aligned 0 times concordantly or discordantly; of these: 9410568 mates make up the pairs; of these: 5122953 (54.44%) aligned 0 times 3510835 (37.31%) aligned exactly 1 time 776780 (8.25%) aligned >1 times 66.98% overall alignment rate Time searching: 00:04:15 Overall time: 00:04:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 158467 / 3051506 = 0.0519 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:18:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:18:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:18:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:18:12: 1000000 INFO @ Sat, 15 Jan 2022 19:18:16: 2000000 INFO @ Sat, 15 Jan 2022 19:18:21: 3000000 INFO @ Sat, 15 Jan 2022 19:18:25: 4000000 INFO @ Sat, 15 Jan 2022 19:18:30: 5000000 INFO @ Sat, 15 Jan 2022 19:18:34: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:18:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:18:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:18:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:18:39: 7000000 INFO @ Sat, 15 Jan 2022 19:18:43: 1000000 INFO @ Sat, 15 Jan 2022 19:18:45: 8000000 INFO @ Sat, 15 Jan 2022 19:18:49: 2000000 INFO @ Sat, 15 Jan 2022 19:18:50: 9000000 INFO @ Sat, 15 Jan 2022 19:18:54: 3000000 INFO @ Sat, 15 Jan 2022 19:18:56: 10000000 INFO @ Sat, 15 Jan 2022 19:18:56: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:18:56: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:18:56: #1 total tags in treatment: 2873931 INFO @ Sat, 15 Jan 2022 19:18:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:18:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:18:56: #1 tags after filtering in treatment: 2324668 INFO @ Sat, 15 Jan 2022 19:18:56: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 19:18:56: #1 finished! INFO @ Sat, 15 Jan 2022 19:18:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:18:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:18:56: #2 number of paired peaks: 59 WARNING @ Sat, 15 Jan 2022 19:18:56: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:18:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:19:00: 4000000 INFO @ Sat, 15 Jan 2022 19:19:05: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:19:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:19:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:19:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:19:10: 6000000 INFO @ Sat, 15 Jan 2022 19:19:13: 1000000 INFO @ Sat, 15 Jan 2022 19:19:16: 7000000 INFO @ Sat, 15 Jan 2022 19:19:18: 2000000 INFO @ Sat, 15 Jan 2022 19:19:21: 8000000 INFO @ Sat, 15 Jan 2022 19:19:23: 3000000 INFO @ Sat, 15 Jan 2022 19:19:26: 9000000 INFO @ Sat, 15 Jan 2022 19:19:29: 4000000 INFO @ Sat, 15 Jan 2022 19:19:32: 10000000 INFO @ Sat, 15 Jan 2022 19:19:32: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:19:32: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:19:32: #1 total tags in treatment: 2873931 INFO @ Sat, 15 Jan 2022 19:19:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:19:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:19:32: #1 tags after filtering in treatment: 2324668 INFO @ Sat, 15 Jan 2022 19:19:32: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 19:19:32: #1 finished! INFO @ Sat, 15 Jan 2022 19:19:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:19:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:19:32: #2 number of paired peaks: 59 WARNING @ Sat, 15 Jan 2022 19:19:32: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:19:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:19:34: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:19:39: 6000000 INFO @ Sat, 15 Jan 2022 19:19:44: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:19:49: 8000000 INFO @ Sat, 15 Jan 2022 19:19:54: 9000000 INFO @ Sat, 15 Jan 2022 19:19:59: 10000000 INFO @ Sat, 15 Jan 2022 19:19:59: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:19:59: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:19:59: #1 total tags in treatment: 2873931 INFO @ Sat, 15 Jan 2022 19:19:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:19:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:19:59: #1 tags after filtering in treatment: 2324668 INFO @ Sat, 15 Jan 2022 19:19:59: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 19:19:59: #1 finished! INFO @ Sat, 15 Jan 2022 19:19:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:19:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:20:00: #2 number of paired peaks: 59 WARNING @ Sat, 15 Jan 2022 19:20:00: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:20:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781156/SRX11781156.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling