Job ID = 14520412 SRX = SRX11781136 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:02:58 prefetch.2.10.7: 1) Downloading 'SRR15481079'... 2022-01-15T10:02:59 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:03:18 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:03:19 prefetch.2.10.7: 'SRR15481079' is valid 2022-01-15T10:03:19 prefetch.2.10.7: 1) 'SRR15481079' was downloaded successfully 2022-01-15T10:03:19 prefetch.2.10.7: 'SRR15481079' has 0 unresolved dependencies Read 8618452 spots for SRR15481079/SRR15481079.sra Written 8618452 spots for SRR15481079/SRR15481079.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:55 8618452 reads; of these: 8618452 (100.00%) were paired; of these: 4959583 (57.55%) aligned concordantly 0 times 3096297 (35.93%) aligned concordantly exactly 1 time 562572 (6.53%) aligned concordantly >1 times ---- 4959583 pairs aligned concordantly 0 times; of these: 32153 (0.65%) aligned discordantly 1 time ---- 4927430 pairs aligned 0 times concordantly or discordantly; of these: 9854860 mates make up the pairs; of these: 5346522 (54.25%) aligned 0 times 3775443 (38.31%) aligned exactly 1 time 732895 (7.44%) aligned >1 times 68.98% overall alignment rate Time searching: 00:08:55 Overall time: 00:08:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 152756 / 3690220 = 0.0414 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:19:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:19:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:19:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:19:18: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:19:32: 2000000 INFO @ Sat, 15 Jan 2022 19:19:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:19:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:19:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:19:44: 3000000 INFO @ Sat, 15 Jan 2022 19:19:48: 1000000 INFO @ Sat, 15 Jan 2022 19:19:54: 4000000 INFO @ Sat, 15 Jan 2022 19:19:59: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:20:05: 5000000 INFO @ Sat, 15 Jan 2022 19:20:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:20:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:20:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:20:10: 3000000 INFO @ Sat, 15 Jan 2022 19:20:15: 6000000 INFO @ Sat, 15 Jan 2022 19:20:16: 1000000 INFO @ Sat, 15 Jan 2022 19:20:22: 4000000 INFO @ Sat, 15 Jan 2022 19:20:27: 7000000 INFO @ Sat, 15 Jan 2022 19:20:28: 2000000 INFO @ Sat, 15 Jan 2022 19:20:33: 5000000 INFO @ Sat, 15 Jan 2022 19:20:40: 8000000 INFO @ Sat, 15 Jan 2022 19:20:41: 3000000 INFO @ Sat, 15 Jan 2022 19:20:47: 6000000 INFO @ Sat, 15 Jan 2022 19:20:54: 9000000 INFO @ Sat, 15 Jan 2022 19:20:55: 4000000 INFO @ Sat, 15 Jan 2022 19:21:00: 7000000 INFO @ Sat, 15 Jan 2022 19:21:07: 10000000 INFO @ Sat, 15 Jan 2022 19:21:08: 5000000 INFO @ Sat, 15 Jan 2022 19:21:13: 8000000 INFO @ Sat, 15 Jan 2022 19:21:19: 11000000 INFO @ Sat, 15 Jan 2022 19:21:19: 6000000 INFO @ Sat, 15 Jan 2022 19:21:24: 9000000 INFO @ Sat, 15 Jan 2022 19:21:26: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:21:26: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:21:26: #1 total tags in treatment: 3506886 INFO @ Sat, 15 Jan 2022 19:21:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:21:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:21:26: #1 tags after filtering in treatment: 2813546 INFO @ Sat, 15 Jan 2022 19:21:26: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 19:21:26: #1 finished! INFO @ Sat, 15 Jan 2022 19:21:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:21:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:21:26: #2 number of paired peaks: 40 WARNING @ Sat, 15 Jan 2022 19:21:26: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:21:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:21:33: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:21:36: 10000000 INFO @ Sat, 15 Jan 2022 19:21:44: 8000000 INFO @ Sat, 15 Jan 2022 19:21:48: 11000000 INFO @ Sat, 15 Jan 2022 19:21:53: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:21:53: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:21:53: #1 total tags in treatment: 3506886 INFO @ Sat, 15 Jan 2022 19:21:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:21:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:21:54: #1 tags after filtering in treatment: 2813546 INFO @ Sat, 15 Jan 2022 19:21:54: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 19:21:54: #1 finished! INFO @ Sat, 15 Jan 2022 19:21:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:21:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:21:54: #2 number of paired peaks: 40 WARNING @ Sat, 15 Jan 2022 19:21:54: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:21:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:21:54: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:22:05: 10000000 INFO @ Sat, 15 Jan 2022 19:22:15: 11000000 INFO @ Sat, 15 Jan 2022 19:22:21: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:22:21: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:22:21: #1 total tags in treatment: 3506886 INFO @ Sat, 15 Jan 2022 19:22:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:22:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:22:21: #1 tags after filtering in treatment: 2813546 INFO @ Sat, 15 Jan 2022 19:22:21: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 19:22:21: #1 finished! INFO @ Sat, 15 Jan 2022 19:22:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:22:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:22:21: #2 number of paired peaks: 40 WARNING @ Sat, 15 Jan 2022 19:22:21: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:22:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781136/SRX11781136.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling