Job ID = 14520394 SRX = SRX11781124 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:01:37 prefetch.2.10.7: 1) Downloading 'SRR15481067'... 2022-01-15T10:01:37 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:02:11 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:02:12 prefetch.2.10.7: 'SRR15481067' is valid 2022-01-15T10:02:12 prefetch.2.10.7: 1) 'SRR15481067' was downloaded successfully 2022-01-15T10:02:12 prefetch.2.10.7: 'SRR15481067' has 0 unresolved dependencies Read 8810324 spots for SRR15481067/SRR15481067.sra Written 8810324 spots for SRR15481067/SRR15481067.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:44 8810324 reads; of these: 8810324 (100.00%) were paired; of these: 5465870 (62.04%) aligned concordantly 0 times 2868275 (32.56%) aligned concordantly exactly 1 time 476179 (5.40%) aligned concordantly >1 times ---- 5465870 pairs aligned concordantly 0 times; of these: 18723 (0.34%) aligned discordantly 1 time ---- 5447147 pairs aligned 0 times concordantly or discordantly; of these: 10894294 mates make up the pairs; of these: 5822624 (53.45%) aligned 0 times 4313459 (39.59%) aligned exactly 1 time 758211 (6.96%) aligned >1 times 66.96% overall alignment rate Time searching: 00:06:44 Overall time: 00:06:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 143786 / 3362225 = 0.0428 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:13:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:13:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:13:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:13:24: 1000000 INFO @ Sat, 15 Jan 2022 19:13:30: 2000000 INFO @ Sat, 15 Jan 2022 19:13:35: 3000000 INFO @ Sat, 15 Jan 2022 19:13:40: 4000000 INFO @ Sat, 15 Jan 2022 19:13:45: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:13:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:13:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:13:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:13:51: 6000000 INFO @ Sat, 15 Jan 2022 19:13:54: 1000000 INFO @ Sat, 15 Jan 2022 19:13:56: 7000000 INFO @ Sat, 15 Jan 2022 19:14:00: 2000000 INFO @ Sat, 15 Jan 2022 19:14:02: 8000000 INFO @ Sat, 15 Jan 2022 19:14:06: 3000000 INFO @ Sat, 15 Jan 2022 19:14:08: 9000000 INFO @ Sat, 15 Jan 2022 19:14:11: 4000000 INFO @ Sat, 15 Jan 2022 19:14:13: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:14:17: 5000000 INFO @ Sat, 15 Jan 2022 19:14:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:14:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:14:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:14:19: 11000000 INFO @ Sat, 15 Jan 2022 19:14:22: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:14:22: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:14:22: #1 total tags in treatment: 3201116 INFO @ Sat, 15 Jan 2022 19:14:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:14:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:14:22: #1 tags after filtering in treatment: 2630890 INFO @ Sat, 15 Jan 2022 19:14:22: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 19:14:22: #1 finished! INFO @ Sat, 15 Jan 2022 19:14:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:14:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:14:22: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 19:14:22: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:14:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:14:23: 6000000 INFO @ Sat, 15 Jan 2022 19:14:24: 1000000 INFO @ Sat, 15 Jan 2022 19:14:29: 7000000 INFO @ Sat, 15 Jan 2022 19:14:30: 2000000 INFO @ Sat, 15 Jan 2022 19:14:34: 8000000 INFO @ Sat, 15 Jan 2022 19:14:36: 3000000 INFO @ Sat, 15 Jan 2022 19:14:40: 9000000 INFO @ Sat, 15 Jan 2022 19:14:41: 4000000 INFO @ Sat, 15 Jan 2022 19:14:46: 10000000 INFO @ Sat, 15 Jan 2022 19:14:47: 5000000 INFO @ Sat, 15 Jan 2022 19:14:52: 11000000 INFO @ Sat, 15 Jan 2022 19:14:53: 6000000 INFO @ Sat, 15 Jan 2022 19:14:54: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:14:54: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:14:54: #1 total tags in treatment: 3201116 INFO @ Sat, 15 Jan 2022 19:14:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:14:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:14:54: #1 tags after filtering in treatment: 2630890 INFO @ Sat, 15 Jan 2022 19:14:54: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 19:14:54: #1 finished! INFO @ Sat, 15 Jan 2022 19:14:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:14:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:14:55: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 19:14:55: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:14:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:14:58: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:15:03: 8000000 INFO @ Sat, 15 Jan 2022 19:15:08: 9000000 INFO @ Sat, 15 Jan 2022 19:15:14: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:15:19: 11000000 INFO @ Sat, 15 Jan 2022 19:15:21: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 19:15:21: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 19:15:21: #1 total tags in treatment: 3201116 INFO @ Sat, 15 Jan 2022 19:15:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:15:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:15:21: #1 tags after filtering in treatment: 2630890 INFO @ Sat, 15 Jan 2022 19:15:21: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 19:15:21: #1 finished! INFO @ Sat, 15 Jan 2022 19:15:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:15:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:15:21: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 19:15:21: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:15:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781124/SRX11781124.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling