Job ID = 14520356 SRX = SRX11781103 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:59:51 prefetch.2.10.7: 1) Downloading 'SRR15481046'... 2022-01-15T09:59:51 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:01:43 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:01:43 prefetch.2.10.7: 1) 'SRR15481046' was downloaded successfully 2022-01-15T10:01:43 prefetch.2.10.7: 'SRR15481046' has 0 unresolved dependencies Read 22448965 spots for SRR15481046/SRR15481046.sra Written 22448965 spots for SRR15481046/SRR15481046.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:16 22448965 reads; of these: 22448965 (100.00%) were paired; of these: 5096541 (22.70%) aligned concordantly 0 times 15337248 (68.32%) aligned concordantly exactly 1 time 2015176 (8.98%) aligned concordantly >1 times ---- 5096541 pairs aligned concordantly 0 times; of these: 829782 (16.28%) aligned discordantly 1 time ---- 4266759 pairs aligned 0 times concordantly or discordantly; of these: 8533518 mates make up the pairs; of these: 4698269 (55.06%) aligned 0 times 3142668 (36.83%) aligned exactly 1 time 692581 (8.12%) aligned >1 times 89.54% overall alignment rate Time searching: 00:17:16 Overall time: 00:17:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4058572 / 18179198 = 0.2233 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:34:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:34:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:34:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:34:59: 1000000 INFO @ Sat, 15 Jan 2022 19:35:06: 2000000 INFO @ Sat, 15 Jan 2022 19:35:14: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:35:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:35:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:35:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:35:23: 4000000 INFO @ Sat, 15 Jan 2022 19:35:29: 1000000 INFO @ Sat, 15 Jan 2022 19:35:32: 5000000 INFO @ Sat, 15 Jan 2022 19:35:37: 2000000 INFO @ Sat, 15 Jan 2022 19:35:41: 6000000 INFO @ Sat, 15 Jan 2022 19:35:45: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:35:50: 7000000 INFO @ Sat, 15 Jan 2022 19:35:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:35:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:35:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:35:53: 4000000 INFO @ Sat, 15 Jan 2022 19:35:58: 8000000 INFO @ Sat, 15 Jan 2022 19:35:59: 1000000 INFO @ Sat, 15 Jan 2022 19:36:02: 5000000 INFO @ Sat, 15 Jan 2022 19:36:07: 9000000 INFO @ Sat, 15 Jan 2022 19:36:08: 2000000 INFO @ Sat, 15 Jan 2022 19:36:10: 6000000 INFO @ Sat, 15 Jan 2022 19:36:16: 10000000 INFO @ Sat, 15 Jan 2022 19:36:17: 3000000 INFO @ Sat, 15 Jan 2022 19:36:18: 7000000 INFO @ Sat, 15 Jan 2022 19:36:25: 11000000 INFO @ Sat, 15 Jan 2022 19:36:26: 4000000 INFO @ Sat, 15 Jan 2022 19:36:26: 8000000 INFO @ Sat, 15 Jan 2022 19:36:32: 12000000 INFO @ Sat, 15 Jan 2022 19:36:34: 9000000 INFO @ Sat, 15 Jan 2022 19:36:34: 5000000 INFO @ Sat, 15 Jan 2022 19:36:41: 13000000 INFO @ Sat, 15 Jan 2022 19:36:42: 10000000 INFO @ Sat, 15 Jan 2022 19:36:43: 6000000 INFO @ Sat, 15 Jan 2022 19:36:50: 14000000 INFO @ Sat, 15 Jan 2022 19:36:50: 11000000 INFO @ Sat, 15 Jan 2022 19:36:52: 7000000 INFO @ Sat, 15 Jan 2022 19:36:58: 15000000 INFO @ Sat, 15 Jan 2022 19:36:59: 12000000 INFO @ Sat, 15 Jan 2022 19:37:01: 8000000 INFO @ Sat, 15 Jan 2022 19:37:07: 13000000 INFO @ Sat, 15 Jan 2022 19:37:07: 16000000 INFO @ Sat, 15 Jan 2022 19:37:10: 9000000 INFO @ Sat, 15 Jan 2022 19:37:15: 14000000 INFO @ Sat, 15 Jan 2022 19:37:16: 17000000 INFO @ Sat, 15 Jan 2022 19:37:19: 10000000 INFO @ Sat, 15 Jan 2022 19:37:23: 15000000 INFO @ Sat, 15 Jan 2022 19:37:25: 18000000 INFO @ Sat, 15 Jan 2022 19:37:28: 11000000 INFO @ Sat, 15 Jan 2022 19:37:32: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:37:34: 19000000 INFO @ Sat, 15 Jan 2022 19:37:35: 12000000 INFO @ Sat, 15 Jan 2022 19:37:40: 17000000 INFO @ Sat, 15 Jan 2022 19:37:42: 20000000 INFO @ Sat, 15 Jan 2022 19:37:44: 13000000 INFO @ Sat, 15 Jan 2022 19:37:48: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:37:51: 21000000 INFO @ Sat, 15 Jan 2022 19:37:53: 14000000 INFO @ Sat, 15 Jan 2022 19:37:56: 19000000 INFO @ Sat, 15 Jan 2022 19:38:00: 22000000 INFO @ Sat, 15 Jan 2022 19:38:02: 15000000 INFO @ Sat, 15 Jan 2022 19:38:04: 20000000 INFO @ Sat, 15 Jan 2022 19:38:09: 23000000 INFO @ Sat, 15 Jan 2022 19:38:11: 16000000 INFO @ Sat, 15 Jan 2022 19:38:12: 21000000 INFO @ Sat, 15 Jan 2022 19:38:18: 24000000 INFO @ Sat, 15 Jan 2022 19:38:20: 22000000 INFO @ Sat, 15 Jan 2022 19:38:20: 17000000 INFO @ Sat, 15 Jan 2022 19:38:27: 25000000 INFO @ Sat, 15 Jan 2022 19:38:28: 23000000 INFO @ Sat, 15 Jan 2022 19:38:30: 18000000 INFO @ Sat, 15 Jan 2022 19:38:36: 26000000 INFO @ Sat, 15 Jan 2022 19:38:36: 24000000 INFO @ Sat, 15 Jan 2022 19:38:38: 19000000 INFO @ Sat, 15 Jan 2022 19:38:44: 25000000 INFO @ Sat, 15 Jan 2022 19:38:45: 27000000 INFO @ Sat, 15 Jan 2022 19:38:47: 20000000 INFO @ Sat, 15 Jan 2022 19:38:52: 26000000 INFO @ Sat, 15 Jan 2022 19:38:54: 28000000 INFO @ Sat, 15 Jan 2022 19:38:56: 21000000 INFO @ Sat, 15 Jan 2022 19:39:00: 27000000 INFO @ Sat, 15 Jan 2022 19:39:02: 29000000 INFO @ Sat, 15 Jan 2022 19:39:05: 22000000 INFO @ Sat, 15 Jan 2022 19:39:09: 28000000 INFO @ Sat, 15 Jan 2022 19:39:11: 30000000 INFO @ Sat, 15 Jan 2022 19:39:13: 23000000 INFO @ Sat, 15 Jan 2022 19:39:17: 29000000 INFO @ Sat, 15 Jan 2022 19:39:20: 31000000 INFO @ Sat, 15 Jan 2022 19:39:22: 24000000 INFO @ Sat, 15 Jan 2022 19:39:25: 30000000 INFO @ Sat, 15 Jan 2022 19:39:29: 32000000 INFO @ Sat, 15 Jan 2022 19:39:30: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:39:30: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:39:30: #1 total tags in treatment: 13406565 INFO @ Sat, 15 Jan 2022 19:39:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:39:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:39:30: #1 tags after filtering in treatment: 7788286 INFO @ Sat, 15 Jan 2022 19:39:30: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 19:39:30: #1 finished! INFO @ Sat, 15 Jan 2022 19:39:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:39:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:39:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:39:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:39:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:39:31: 25000000 INFO @ Sat, 15 Jan 2022 19:39:33: 31000000 INFO @ Sat, 15 Jan 2022 19:39:39: 26000000 INFO @ Sat, 15 Jan 2022 19:39:41: 32000000 INFO @ Sat, 15 Jan 2022 19:39:41: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:39:41: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:39:41: #1 total tags in treatment: 13406565 INFO @ Sat, 15 Jan 2022 19:39:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:39:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:39:42: #1 tags after filtering in treatment: 7788286 INFO @ Sat, 15 Jan 2022 19:39:42: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 19:39:42: #1 finished! INFO @ Sat, 15 Jan 2022 19:39:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:39:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:39:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:39:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:39:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:39:47: 27000000 INFO @ Sat, 15 Jan 2022 19:39:55: 28000000 INFO @ Sat, 15 Jan 2022 19:40:02: 29000000 INFO @ Sat, 15 Jan 2022 19:40:10: 30000000 INFO @ Sat, 15 Jan 2022 19:40:17: 31000000 INFO @ Sat, 15 Jan 2022 19:40:25: 32000000 INFO @ Sat, 15 Jan 2022 19:40:26: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:40:26: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:40:26: #1 total tags in treatment: 13406565 INFO @ Sat, 15 Jan 2022 19:40:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:40:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:40:26: #1 tags after filtering in treatment: 7788286 INFO @ Sat, 15 Jan 2022 19:40:26: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 19:40:26: #1 finished! INFO @ Sat, 15 Jan 2022 19:40:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:40:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:40:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:40:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:40:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781103/SRX11781103.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling