Job ID = 14520351 SRX = SRX11781102 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:59:51 prefetch.2.10.7: 1) Downloading 'SRR15481045'... 2022-01-15T09:59:51 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:00:56 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:00:56 prefetch.2.10.7: 1) 'SRR15481045' was downloaded successfully 2022-01-15T10:00:56 prefetch.2.10.7: 'SRR15481045' has 0 unresolved dependencies Read 14729781 spots for SRR15481045/SRR15481045.sra Written 14729781 spots for SRR15481045/SRR15481045.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:33 14729781 reads; of these: 14729781 (100.00%) were paired; of these: 2319782 (15.75%) aligned concordantly 0 times 10501158 (71.29%) aligned concordantly exactly 1 time 1908841 (12.96%) aligned concordantly >1 times ---- 2319782 pairs aligned concordantly 0 times; of these: 126803 (5.47%) aligned discordantly 1 time ---- 2192979 pairs aligned 0 times concordantly or discordantly; of these: 4385958 mates make up the pairs; of these: 2667295 (60.81%) aligned 0 times 1401431 (31.95%) aligned exactly 1 time 317232 (7.23%) aligned >1 times 90.95% overall alignment rate Time searching: 00:14:33 Overall time: 00:14:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 879162 / 12535340 = 0.0701 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:33:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:33:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:33:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:33:42: 1000000 INFO @ Sat, 15 Jan 2022 19:33:47: 2000000 INFO @ Sat, 15 Jan 2022 19:33:52: 3000000 INFO @ Sat, 15 Jan 2022 19:33:56: 4000000 INFO @ Sat, 15 Jan 2022 19:34:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:34:06: 6000000 INFO @ Sat, 15 Jan 2022 19:34:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:34:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:34:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:34:11: 7000000 INFO @ Sat, 15 Jan 2022 19:34:13: 1000000 INFO @ Sat, 15 Jan 2022 19:34:16: 8000000 INFO @ Sat, 15 Jan 2022 19:34:19: 2000000 INFO @ Sat, 15 Jan 2022 19:34:21: 9000000 INFO @ Sat, 15 Jan 2022 19:34:25: 3000000 INFO @ Sat, 15 Jan 2022 19:34:26: 10000000 INFO @ Sat, 15 Jan 2022 19:34:31: 11000000 INFO @ Sat, 15 Jan 2022 19:34:31: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:34:35: 12000000 INFO @ Sat, 15 Jan 2022 19:34:37: 5000000 INFO @ Sat, 15 Jan 2022 19:34:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:34:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:34:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:34:40: 13000000 INFO @ Sat, 15 Jan 2022 19:34:42: 1000000 INFO @ Sat, 15 Jan 2022 19:34:43: 6000000 INFO @ Sat, 15 Jan 2022 19:34:45: 14000000 INFO @ Sat, 15 Jan 2022 19:34:47: 2000000 INFO @ Sat, 15 Jan 2022 19:34:48: 7000000 INFO @ Sat, 15 Jan 2022 19:34:50: 15000000 INFO @ Sat, 15 Jan 2022 19:34:52: 3000000 INFO @ Sat, 15 Jan 2022 19:34:54: 8000000 INFO @ Sat, 15 Jan 2022 19:34:55: 16000000 INFO @ Sat, 15 Jan 2022 19:34:57: 4000000 INFO @ Sat, 15 Jan 2022 19:34:59: 17000000 INFO @ Sat, 15 Jan 2022 19:35:00: 9000000 INFO @ Sat, 15 Jan 2022 19:35:02: 5000000 INFO @ Sat, 15 Jan 2022 19:35:04: 18000000 INFO @ Sat, 15 Jan 2022 19:35:06: 10000000 INFO @ Sat, 15 Jan 2022 19:35:06: 6000000 INFO @ Sat, 15 Jan 2022 19:35:09: 19000000 INFO @ Sat, 15 Jan 2022 19:35:11: 7000000 INFO @ Sat, 15 Jan 2022 19:35:11: 11000000 INFO @ Sat, 15 Jan 2022 19:35:14: 20000000 INFO @ Sat, 15 Jan 2022 19:35:16: 8000000 INFO @ Sat, 15 Jan 2022 19:35:17: 12000000 INFO @ Sat, 15 Jan 2022 19:35:19: 21000000 INFO @ Sat, 15 Jan 2022 19:35:21: 9000000 INFO @ Sat, 15 Jan 2022 19:35:23: 13000000 INFO @ Sat, 15 Jan 2022 19:35:23: 22000000 INFO @ Sat, 15 Jan 2022 19:35:26: 10000000 INFO @ Sat, 15 Jan 2022 19:35:28: 23000000 INFO @ Sat, 15 Jan 2022 19:35:29: 14000000 INFO @ Sat, 15 Jan 2022 19:35:31: 11000000 INFO @ Sat, 15 Jan 2022 19:35:33: 24000000 INFO @ Sat, 15 Jan 2022 19:35:34: 15000000 INFO @ Sat, 15 Jan 2022 19:35:36: 12000000 INFO @ Sat, 15 Jan 2022 19:35:38: 25000000 INFO @ Sat, 15 Jan 2022 19:35:38: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:35:38: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:35:38: #1 total tags in treatment: 11535448 INFO @ Sat, 15 Jan 2022 19:35:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:35:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:35:38: #1 tags after filtering in treatment: 7539955 INFO @ Sat, 15 Jan 2022 19:35:38: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 19:35:38: #1 finished! INFO @ Sat, 15 Jan 2022 19:35:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:35:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:35:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:35:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:35:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:35:40: 16000000 INFO @ Sat, 15 Jan 2022 19:35:41: 13000000 INFO @ Sat, 15 Jan 2022 19:35:45: 17000000 INFO @ Sat, 15 Jan 2022 19:35:45: 14000000 INFO @ Sat, 15 Jan 2022 19:35:50: 15000000 INFO @ Sat, 15 Jan 2022 19:35:51: 18000000 INFO @ Sat, 15 Jan 2022 19:35:55: 16000000 INFO @ Sat, 15 Jan 2022 19:35:57: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:36:00: 17000000 INFO @ Sat, 15 Jan 2022 19:36:02: 20000000 INFO @ Sat, 15 Jan 2022 19:36:04: 18000000 INFO @ Sat, 15 Jan 2022 19:36:08: 21000000 INFO @ Sat, 15 Jan 2022 19:36:09: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:36:13: 22000000 INFO @ Sat, 15 Jan 2022 19:36:14: 20000000 INFO @ Sat, 15 Jan 2022 19:36:19: 21000000 INFO @ Sat, 15 Jan 2022 19:36:19: 23000000 INFO @ Sat, 15 Jan 2022 19:36:23: 22000000 INFO @ Sat, 15 Jan 2022 19:36:25: 24000000 INFO @ Sat, 15 Jan 2022 19:36:28: 23000000 INFO @ Sat, 15 Jan 2022 19:36:30: 25000000 INFO @ Sat, 15 Jan 2022 19:36:31: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:36:31: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:36:31: #1 total tags in treatment: 11535448 INFO @ Sat, 15 Jan 2022 19:36:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:36:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:36:31: #1 tags after filtering in treatment: 7539955 INFO @ Sat, 15 Jan 2022 19:36:31: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 19:36:31: #1 finished! INFO @ Sat, 15 Jan 2022 19:36:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:36:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:36:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:36:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:36:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:36:33: 24000000 INFO @ Sat, 15 Jan 2022 19:36:38: 25000000 INFO @ Sat, 15 Jan 2022 19:36:38: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:36:38: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:36:38: #1 total tags in treatment: 11535448 INFO @ Sat, 15 Jan 2022 19:36:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:36:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:36:38: #1 tags after filtering in treatment: 7539955 INFO @ Sat, 15 Jan 2022 19:36:38: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 19:36:38: #1 finished! INFO @ Sat, 15 Jan 2022 19:36:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:36:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:36:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:36:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:36:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781102/SRX11781102.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling