Job ID = 14520350 SRX = SRX11781101 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:59:51 prefetch.2.10.7: 1) Downloading 'SRR15481044'... 2022-01-15T09:59:51 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:01:27 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:01:27 prefetch.2.10.7: 1) 'SRR15481044' was downloaded successfully 2022-01-15T10:01:27 prefetch.2.10.7: 'SRR15481044' has 0 unresolved dependencies Read 16674861 spots for SRR15481044/SRR15481044.sra Written 16674861 spots for SRR15481044/SRR15481044.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:08 16674861 reads; of these: 16674861 (100.00%) were paired; of these: 2981474 (17.88%) aligned concordantly 0 times 12434935 (74.57%) aligned concordantly exactly 1 time 1258452 (7.55%) aligned concordantly >1 times ---- 2981474 pairs aligned concordantly 0 times; of these: 405322 (13.59%) aligned discordantly 1 time ---- 2576152 pairs aligned 0 times concordantly or discordantly; of these: 5152304 mates make up the pairs; of these: 2992671 (58.08%) aligned 0 times 1853654 (35.98%) aligned exactly 1 time 305979 (5.94%) aligned >1 times 91.03% overall alignment rate Time searching: 00:13:08 Overall time: 00:13:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3278554 / 14097129 = 0.2326 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:27:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:27:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:27:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:28:03: 1000000 INFO @ Sat, 15 Jan 2022 19:28:10: 2000000 INFO @ Sat, 15 Jan 2022 19:28:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:28:25: 4000000 INFO @ Sat, 15 Jan 2022 19:28:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:28:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:28:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:28:33: 1000000 INFO @ Sat, 15 Jan 2022 19:28:34: 5000000 INFO @ Sat, 15 Jan 2022 19:28:41: 2000000 INFO @ Sat, 15 Jan 2022 19:28:42: 6000000 INFO @ Sat, 15 Jan 2022 19:28:48: 3000000 INFO @ Sat, 15 Jan 2022 19:28:50: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:28:55: 4000000 INFO @ Sat, 15 Jan 2022 19:28:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:28:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:28:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:28:58: 8000000 INFO @ Sat, 15 Jan 2022 19:29:03: 5000000 INFO @ Sat, 15 Jan 2022 19:29:04: 1000000 INFO @ Sat, 15 Jan 2022 19:29:07: 9000000 INFO @ Sat, 15 Jan 2022 19:29:10: 6000000 INFO @ Sat, 15 Jan 2022 19:29:12: 2000000 INFO @ Sat, 15 Jan 2022 19:29:15: 10000000 INFO @ Sat, 15 Jan 2022 19:29:18: 7000000 INFO @ Sat, 15 Jan 2022 19:29:21: 3000000 INFO @ Sat, 15 Jan 2022 19:29:23: 11000000 INFO @ Sat, 15 Jan 2022 19:29:25: 8000000 INFO @ Sat, 15 Jan 2022 19:29:29: 4000000 INFO @ Sat, 15 Jan 2022 19:29:32: 12000000 INFO @ Sat, 15 Jan 2022 19:29:33: 9000000 INFO @ Sat, 15 Jan 2022 19:29:37: 5000000 INFO @ Sat, 15 Jan 2022 19:29:40: 13000000 INFO @ Sat, 15 Jan 2022 19:29:40: 10000000 INFO @ Sat, 15 Jan 2022 19:29:45: 6000000 INFO @ Sat, 15 Jan 2022 19:29:48: 11000000 INFO @ Sat, 15 Jan 2022 19:29:48: 14000000 INFO @ Sat, 15 Jan 2022 19:29:52: 7000000 INFO @ Sat, 15 Jan 2022 19:29:55: 12000000 INFO @ Sat, 15 Jan 2022 19:29:57: 15000000 INFO @ Sat, 15 Jan 2022 19:30:00: 8000000 INFO @ Sat, 15 Jan 2022 19:30:03: 13000000 INFO @ Sat, 15 Jan 2022 19:30:05: 16000000 INFO @ Sat, 15 Jan 2022 19:30:08: 9000000 INFO @ Sat, 15 Jan 2022 19:30:11: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:30:14: 17000000 INFO @ Sat, 15 Jan 2022 19:30:15: 10000000 INFO @ Sat, 15 Jan 2022 19:30:18: 15000000 INFO @ Sat, 15 Jan 2022 19:30:22: 18000000 INFO @ Sat, 15 Jan 2022 19:30:23: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:30:26: 16000000 INFO @ Sat, 15 Jan 2022 19:30:30: 19000000 INFO @ Sat, 15 Jan 2022 19:30:30: 12000000 INFO @ Sat, 15 Jan 2022 19:30:34: 17000000 INFO @ Sat, 15 Jan 2022 19:30:38: 13000000 INFO @ Sat, 15 Jan 2022 19:30:39: 20000000 INFO @ Sat, 15 Jan 2022 19:30:41: 18000000 INFO @ Sat, 15 Jan 2022 19:30:46: 14000000 INFO @ Sat, 15 Jan 2022 19:30:47: 21000000 INFO @ Sat, 15 Jan 2022 19:30:49: 19000000 INFO @ Sat, 15 Jan 2022 19:30:54: 15000000 INFO @ Sat, 15 Jan 2022 19:30:55: 22000000 INFO @ Sat, 15 Jan 2022 19:30:57: 20000000 INFO @ Sat, 15 Jan 2022 19:31:01: 16000000 INFO @ Sat, 15 Jan 2022 19:31:04: 23000000 INFO @ Sat, 15 Jan 2022 19:31:05: 21000000 INFO @ Sat, 15 Jan 2022 19:31:09: 17000000 INFO @ Sat, 15 Jan 2022 19:31:10: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:31:10: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:31:10: #1 total tags in treatment: 10467991 INFO @ Sat, 15 Jan 2022 19:31:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:31:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:31:10: #1 tags after filtering in treatment: 5521369 INFO @ Sat, 15 Jan 2022 19:31:10: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 19:31:10: #1 finished! INFO @ Sat, 15 Jan 2022 19:31:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:31:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:31:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:31:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:31:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:31:12: 22000000 INFO @ Sat, 15 Jan 2022 19:31:16: 18000000 INFO @ Sat, 15 Jan 2022 19:31:19: 23000000 INFO @ Sat, 15 Jan 2022 19:31:23: 19000000 INFO @ Sat, 15 Jan 2022 19:31:25: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:31:25: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:31:25: #1 total tags in treatment: 10467991 INFO @ Sat, 15 Jan 2022 19:31:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:31:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:31:25: #1 tags after filtering in treatment: 5521369 INFO @ Sat, 15 Jan 2022 19:31:25: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 19:31:25: #1 finished! INFO @ Sat, 15 Jan 2022 19:31:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:31:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:31:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:31:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:31:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:31:30: 20000000 INFO @ Sat, 15 Jan 2022 19:31:37: 21000000 INFO @ Sat, 15 Jan 2022 19:31:43: 22000000 INFO @ Sat, 15 Jan 2022 19:31:49: 23000000 INFO @ Sat, 15 Jan 2022 19:31:54: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:31:54: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:31:54: #1 total tags in treatment: 10467991 INFO @ Sat, 15 Jan 2022 19:31:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:31:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:31:54: #1 tags after filtering in treatment: 5521369 INFO @ Sat, 15 Jan 2022 19:31:54: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 19:31:54: #1 finished! INFO @ Sat, 15 Jan 2022 19:31:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:31:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:31:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:31:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:31:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781101/SRX11781101.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling