Job ID = 14520642 SRX = SRX11781099 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:30:18 prefetch.2.10.7: 1) Downloading 'SRR15481042'... 2022-01-15T10:30:18 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:31:58 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:31:58 prefetch.2.10.7: 1) 'SRR15481042' was downloaded successfully 2022-01-15T10:31:58 prefetch.2.10.7: 'SRR15481042' has 0 unresolved dependencies Read 21374713 spots for SRR15481042/SRR15481042.sra Written 21374713 spots for SRR15481042/SRR15481042.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:54 21374713 reads; of these: 21374713 (100.00%) were paired; of these: 7385249 (34.55%) aligned concordantly 0 times 11950331 (55.91%) aligned concordantly exactly 1 time 2039133 (9.54%) aligned concordantly >1 times ---- 7385249 pairs aligned concordantly 0 times; of these: 299184 (4.05%) aligned discordantly 1 time ---- 7086065 pairs aligned 0 times concordantly or discordantly; of these: 14172130 mates make up the pairs; of these: 7544850 (53.24%) aligned 0 times 5495579 (38.78%) aligned exactly 1 time 1131701 (7.99%) aligned >1 times 82.35% overall alignment rate Time searching: 00:15:54 Overall time: 00:15:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2051637 / 14286599 = 0.1436 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:04:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:04:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:04:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:04:37: 1000000 INFO @ Sat, 15 Jan 2022 20:04:43: 2000000 INFO @ Sat, 15 Jan 2022 20:04:50: 3000000 INFO @ Sat, 15 Jan 2022 20:04:56: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:05:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:05:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:05:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:05:02: 5000000 INFO @ Sat, 15 Jan 2022 20:05:08: 1000000 INFO @ Sat, 15 Jan 2022 20:05:09: 6000000 INFO @ Sat, 15 Jan 2022 20:05:14: 2000000 INFO @ Sat, 15 Jan 2022 20:05:15: 7000000 INFO @ Sat, 15 Jan 2022 20:05:21: 3000000 INFO @ Sat, 15 Jan 2022 20:05:22: 8000000 INFO @ Sat, 15 Jan 2022 20:05:28: 4000000 INFO @ Sat, 15 Jan 2022 20:05:28: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:05:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:05:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:05:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:05:34: 5000000 INFO @ Sat, 15 Jan 2022 20:05:35: 10000000 INFO @ Sat, 15 Jan 2022 20:05:37: 1000000 INFO @ Sat, 15 Jan 2022 20:05:41: 6000000 INFO @ Sat, 15 Jan 2022 20:05:41: 11000000 INFO @ Sat, 15 Jan 2022 20:05:43: 2000000 INFO @ Sat, 15 Jan 2022 20:05:48: 7000000 INFO @ Sat, 15 Jan 2022 20:05:48: 12000000 INFO @ Sat, 15 Jan 2022 20:05:49: 3000000 INFO @ Sat, 15 Jan 2022 20:05:54: 8000000 INFO @ Sat, 15 Jan 2022 20:05:54: 13000000 INFO @ Sat, 15 Jan 2022 20:05:55: 4000000 INFO @ Sat, 15 Jan 2022 20:06:01: 9000000 INFO @ Sat, 15 Jan 2022 20:06:01: 5000000 INFO @ Sat, 15 Jan 2022 20:06:01: 14000000 INFO @ Sat, 15 Jan 2022 20:06:07: 6000000 INFO @ Sat, 15 Jan 2022 20:06:07: 10000000 INFO @ Sat, 15 Jan 2022 20:06:08: 15000000 INFO @ Sat, 15 Jan 2022 20:06:13: 7000000 INFO @ Sat, 15 Jan 2022 20:06:14: 11000000 INFO @ Sat, 15 Jan 2022 20:06:14: 16000000 INFO @ Sat, 15 Jan 2022 20:06:18: 8000000 INFO @ Sat, 15 Jan 2022 20:06:20: 12000000 INFO @ Sat, 15 Jan 2022 20:06:21: 17000000 INFO @ Sat, 15 Jan 2022 20:06:24: 9000000 INFO @ Sat, 15 Jan 2022 20:06:27: 13000000 INFO @ Sat, 15 Jan 2022 20:06:28: 18000000 INFO @ Sat, 15 Jan 2022 20:06:30: 10000000 INFO @ Sat, 15 Jan 2022 20:06:34: 14000000 INFO @ Sat, 15 Jan 2022 20:06:35: 19000000 INFO @ Sat, 15 Jan 2022 20:06:36: 11000000 INFO @ Sat, 15 Jan 2022 20:06:40: 15000000 INFO @ Sat, 15 Jan 2022 20:06:41: 20000000 INFO @ Sat, 15 Jan 2022 20:06:42: 12000000 INFO @ Sat, 15 Jan 2022 20:06:47: 16000000 INFO @ Sat, 15 Jan 2022 20:06:48: 13000000 INFO @ Sat, 15 Jan 2022 20:06:48: 21000000 INFO @ Sat, 15 Jan 2022 20:06:54: 17000000 INFO @ Sat, 15 Jan 2022 20:06:54: 14000000 INFO @ Sat, 15 Jan 2022 20:06:54: 22000000 INFO @ Sat, 15 Jan 2022 20:07:00: 15000000 INFO @ Sat, 15 Jan 2022 20:07:00: 18000000 INFO @ Sat, 15 Jan 2022 20:07:01: 23000000 INFO @ Sat, 15 Jan 2022 20:07:06: 16000000 INFO @ Sat, 15 Jan 2022 20:07:07: 19000000 INFO @ Sat, 15 Jan 2022 20:07:08: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:07:12: 17000000 INFO @ Sat, 15 Jan 2022 20:07:14: 20000000 INFO @ Sat, 15 Jan 2022 20:07:14: 25000000 INFO @ Sat, 15 Jan 2022 20:07:17: 18000000 INFO @ Sat, 15 Jan 2022 20:07:21: 21000000 INFO @ Sat, 15 Jan 2022 20:07:21: 26000000 INFO @ Sat, 15 Jan 2022 20:07:23: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:07:27: 22000000 INFO @ Sat, 15 Jan 2022 20:07:28: 27000000 INFO @ Sat, 15 Jan 2022 20:07:29: 20000000 INFO @ Sat, 15 Jan 2022 20:07:34: 23000000 INFO @ Sat, 15 Jan 2022 20:07:35: 21000000 INFO @ Sat, 15 Jan 2022 20:07:35: 28000000 INFO @ Sat, 15 Jan 2022 20:07:41: 24000000 INFO @ Sat, 15 Jan 2022 20:07:41: 22000000 INFO @ Sat, 15 Jan 2022 20:07:42: 29000000 INFO @ Sat, 15 Jan 2022 20:07:47: 23000000 INFO @ Sat, 15 Jan 2022 20:07:47: 25000000 INFO @ Sat, 15 Jan 2022 20:07:49: 30000000 INFO @ Sat, 15 Jan 2022 20:07:53: 24000000 INFO @ Sat, 15 Jan 2022 20:07:54: 26000000 INFO @ Sat, 15 Jan 2022 20:07:56: 31000000 INFO @ Sat, 15 Jan 2022 20:07:57: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:07:57: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:07:57: #1 total tags in treatment: 11958154 INFO @ Sat, 15 Jan 2022 20:07:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:07:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:07:57: #1 tags after filtering in treatment: 7278390 INFO @ Sat, 15 Jan 2022 20:07:57: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 20:07:57: #1 finished! INFO @ Sat, 15 Jan 2022 20:07:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:07:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:07:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:07:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:07:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:07:58: 25000000 INFO @ Sat, 15 Jan 2022 20:08:01: 27000000 INFO @ Sat, 15 Jan 2022 20:08:04: 26000000 INFO @ Sat, 15 Jan 2022 20:08:08: 28000000 INFO @ Sat, 15 Jan 2022 20:08:10: 27000000 INFO @ Sat, 15 Jan 2022 20:08:14: 29000000 INFO @ Sat, 15 Jan 2022 20:08:16: 28000000 INFO @ Sat, 15 Jan 2022 20:08:21: 30000000 INFO @ Sat, 15 Jan 2022 20:08:22: 29000000 INFO @ Sat, 15 Jan 2022 20:08:28: 31000000 INFO @ Sat, 15 Jan 2022 20:08:28: 30000000 INFO @ Sat, 15 Jan 2022 20:08:28: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:08:28: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:08:28: #1 total tags in treatment: 11958154 INFO @ Sat, 15 Jan 2022 20:08:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:08:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:08:29: #1 tags after filtering in treatment: 7278390 INFO @ Sat, 15 Jan 2022 20:08:29: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 20:08:29: #1 finished! INFO @ Sat, 15 Jan 2022 20:08:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:08:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:08:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:08:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:08:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 117 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:08:34: 31000000 INFO @ Sat, 15 Jan 2022 20:08:34: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:08:34: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:08:34: #1 total tags in treatment: 11958154 INFO @ Sat, 15 Jan 2022 20:08:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:08:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:08:35: #1 tags after filtering in treatment: 7278390 INFO @ Sat, 15 Jan 2022 20:08:35: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 20:08:35: #1 finished! INFO @ Sat, 15 Jan 2022 20:08:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:08:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:08:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:08:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:08:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781099/SRX11781099.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling