Job ID = 14520641 SRX = SRX11781098 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:30:18 prefetch.2.10.7: 1) Downloading 'SRR15481041'... 2022-01-15T10:30:18 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:31:47 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:31:47 prefetch.2.10.7: 1) 'SRR15481041' was downloaded successfully 2022-01-15T10:31:47 prefetch.2.10.7: 'SRR15481041' has 0 unresolved dependencies Read 20949215 spots for SRR15481041/SRR15481041.sra Written 20949215 spots for SRR15481041/SRR15481041.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:26 20949215 reads; of these: 20949215 (100.00%) were paired; of these: 3810556 (18.19%) aligned concordantly 0 times 14025079 (66.95%) aligned concordantly exactly 1 time 3113580 (14.86%) aligned concordantly >1 times ---- 3810556 pairs aligned concordantly 0 times; of these: 145265 (3.81%) aligned discordantly 1 time ---- 3665291 pairs aligned 0 times concordantly or discordantly; of these: 7330582 mates make up the pairs; of these: 4135422 (56.41%) aligned 0 times 2540440 (34.66%) aligned exactly 1 time 654720 (8.93%) aligned >1 times 90.13% overall alignment rate Time searching: 00:22:26 Overall time: 00:22:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1189833 / 17281978 = 0.0688 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:12:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:12:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:12:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:12:06: 1000000 INFO @ Sat, 15 Jan 2022 20:12:11: 2000000 INFO @ Sat, 15 Jan 2022 20:12:16: 3000000 INFO @ Sat, 15 Jan 2022 20:12:22: 4000000 INFO @ Sat, 15 Jan 2022 20:12:27: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:12:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:12:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:12:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:12:33: 6000000 INFO @ Sat, 15 Jan 2022 20:12:36: 1000000 INFO @ Sat, 15 Jan 2022 20:12:39: 7000000 INFO @ Sat, 15 Jan 2022 20:12:42: 2000000 INFO @ Sat, 15 Jan 2022 20:12:45: 8000000 INFO @ Sat, 15 Jan 2022 20:12:48: 3000000 INFO @ Sat, 15 Jan 2022 20:12:51: 9000000 INFO @ Sat, 15 Jan 2022 20:12:54: 4000000 INFO @ Sat, 15 Jan 2022 20:12:57: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:13:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:13:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:13:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:13:00: 5000000 INFO @ Sat, 15 Jan 2022 20:13:03: 11000000 INFO @ Sat, 15 Jan 2022 20:13:07: 6000000 INFO @ Sat, 15 Jan 2022 20:13:07: 1000000 INFO @ Sat, 15 Jan 2022 20:13:10: 12000000 INFO @ Sat, 15 Jan 2022 20:13:13: 7000000 INFO @ Sat, 15 Jan 2022 20:13:13: 2000000 INFO @ Sat, 15 Jan 2022 20:13:16: 13000000 INFO @ Sat, 15 Jan 2022 20:13:20: 8000000 INFO @ Sat, 15 Jan 2022 20:13:20: 3000000 INFO @ Sat, 15 Jan 2022 20:13:23: 14000000 INFO @ Sat, 15 Jan 2022 20:13:26: 9000000 INFO @ Sat, 15 Jan 2022 20:13:27: 4000000 INFO @ Sat, 15 Jan 2022 20:13:29: 15000000 INFO @ Sat, 15 Jan 2022 20:13:33: 10000000 INFO @ Sat, 15 Jan 2022 20:13:33: 5000000 INFO @ Sat, 15 Jan 2022 20:13:35: 16000000 INFO @ Sat, 15 Jan 2022 20:13:39: 11000000 INFO @ Sat, 15 Jan 2022 20:13:39: 6000000 INFO @ Sat, 15 Jan 2022 20:13:42: 17000000 INFO @ Sat, 15 Jan 2022 20:13:45: 12000000 INFO @ Sat, 15 Jan 2022 20:13:46: 7000000 INFO @ Sat, 15 Jan 2022 20:13:48: 18000000 INFO @ Sat, 15 Jan 2022 20:13:52: 13000000 INFO @ Sat, 15 Jan 2022 20:13:52: 8000000 INFO @ Sat, 15 Jan 2022 20:13:54: 19000000 INFO @ Sat, 15 Jan 2022 20:13:58: 14000000 INFO @ Sat, 15 Jan 2022 20:13:58: 9000000 INFO @ Sat, 15 Jan 2022 20:14:01: 20000000 INFO @ Sat, 15 Jan 2022 20:14:04: 15000000 INFO @ Sat, 15 Jan 2022 20:14:05: 10000000 INFO @ Sat, 15 Jan 2022 20:14:07: 21000000 INFO @ Sat, 15 Jan 2022 20:14:10: 16000000 INFO @ Sat, 15 Jan 2022 20:14:11: 11000000 INFO @ Sat, 15 Jan 2022 20:14:13: 22000000 INFO @ Sat, 15 Jan 2022 20:14:17: 17000000 INFO @ Sat, 15 Jan 2022 20:14:17: 12000000 INFO @ Sat, 15 Jan 2022 20:14:19: 23000000 INFO @ Sat, 15 Jan 2022 20:14:23: 18000000 INFO @ Sat, 15 Jan 2022 20:14:24: 13000000 INFO @ Sat, 15 Jan 2022 20:14:25: 24000000 INFO @ Sat, 15 Jan 2022 20:14:29: 19000000 INFO @ Sat, 15 Jan 2022 20:14:30: 14000000 INFO @ Sat, 15 Jan 2022 20:14:31: 25000000 INFO @ Sat, 15 Jan 2022 20:14:35: 20000000 INFO @ Sat, 15 Jan 2022 20:14:36: 15000000 INFO @ Sat, 15 Jan 2022 20:14:38: 26000000 INFO @ Sat, 15 Jan 2022 20:14:41: 21000000 INFO @ Sat, 15 Jan 2022 20:14:42: 16000000 INFO @ Sat, 15 Jan 2022 20:14:44: 27000000 INFO @ Sat, 15 Jan 2022 20:14:47: 22000000 INFO @ Sat, 15 Jan 2022 20:14:49: 17000000 INFO @ Sat, 15 Jan 2022 20:14:50: 28000000 INFO @ Sat, 15 Jan 2022 20:14:53: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:14:55: 18000000 INFO @ Sat, 15 Jan 2022 20:14:56: 29000000 INFO @ Sat, 15 Jan 2022 20:14:59: 24000000 INFO @ Sat, 15 Jan 2022 20:15:01: 19000000 INFO @ Sat, 15 Jan 2022 20:15:02: 30000000 INFO @ Sat, 15 Jan 2022 20:15:06: 25000000 INFO @ Sat, 15 Jan 2022 20:15:07: 20000000 INFO @ Sat, 15 Jan 2022 20:15:08: 31000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:15:12: 26000000 INFO @ Sat, 15 Jan 2022 20:15:13: 21000000 INFO @ Sat, 15 Jan 2022 20:15:14: 32000000 INFO @ Sat, 15 Jan 2022 20:15:18: 27000000 INFO @ Sat, 15 Jan 2022 20:15:19: 22000000 INFO @ Sat, 15 Jan 2022 20:15:21: 33000000 INFO @ Sat, 15 Jan 2022 20:15:24: 28000000 INFO @ Sat, 15 Jan 2022 20:15:25: 23000000 INFO @ Sat, 15 Jan 2022 20:15:27: 34000000 INFO @ Sat, 15 Jan 2022 20:15:30: 29000000 INFO @ Sat, 15 Jan 2022 20:15:31: 24000000 INFO @ Sat, 15 Jan 2022 20:15:33: 35000000 INFO @ Sat, 15 Jan 2022 20:15:35: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:15:35: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:15:35: #1 total tags in treatment: 15952993 INFO @ Sat, 15 Jan 2022 20:15:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:15:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:15:36: #1 tags after filtering in treatment: 9284210 INFO @ Sat, 15 Jan 2022 20:15:36: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:15:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:15:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:15:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:15:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:15:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:15:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 20:15:36: 30000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:15:37: 25000000 INFO @ Sat, 15 Jan 2022 20:15:42: 31000000 INFO @ Sat, 15 Jan 2022 20:15:44: 26000000 INFO @ Sat, 15 Jan 2022 20:15:48: 32000000 INFO @ Sat, 15 Jan 2022 20:15:50: 27000000 INFO @ Sat, 15 Jan 2022 20:15:54: 33000000 INFO @ Sat, 15 Jan 2022 20:15:56: 28000000 INFO @ Sat, 15 Jan 2022 20:16:01: 34000000 INFO @ Sat, 15 Jan 2022 20:16:02: 29000000 INFO @ Sat, 15 Jan 2022 20:16:07: 35000000 INFO @ Sat, 15 Jan 2022 20:16:08: 30000000 INFO @ Sat, 15 Jan 2022 20:16:09: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:16:09: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:16:09: #1 total tags in treatment: 15952993 INFO @ Sat, 15 Jan 2022 20:16:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:09: #1 tags after filtering in treatment: 9284210 INFO @ Sat, 15 Jan 2022 20:16:09: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:16:09: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:16:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:16:14: 31000000 INFO @ Sat, 15 Jan 2022 20:16:19: 32000000 INFO @ Sat, 15 Jan 2022 20:16:24: 33000000 INFO @ Sat, 15 Jan 2022 20:16:30: 34000000 INFO @ Sat, 15 Jan 2022 20:16:35: 35000000 INFO @ Sat, 15 Jan 2022 20:16:37: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:16:37: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:16:37: #1 total tags in treatment: 15952993 INFO @ Sat, 15 Jan 2022 20:16:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:38: #1 tags after filtering in treatment: 9284210 INFO @ Sat, 15 Jan 2022 20:16:38: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:16:38: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:16:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781098/SRX11781098.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling