Job ID = 14520640 SRX = SRX11781097 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:29:49 prefetch.2.10.7: 1) Downloading 'SRR15481040'... 2022-01-15T10:29:49 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:31:32 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:31:32 prefetch.2.10.7: 1) 'SRR15481040' was downloaded successfully 2022-01-15T10:31:32 prefetch.2.10.7: 'SRR15481040' has 0 unresolved dependencies Read 21119872 spots for SRR15481040/SRR15481040.sra Written 21119872 spots for SRR15481040/SRR15481040.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:56 21119872 reads; of these: 21119872 (100.00%) were paired; of these: 4410925 (20.89%) aligned concordantly 0 times 14800976 (70.08%) aligned concordantly exactly 1 time 1907971 (9.03%) aligned concordantly >1 times ---- 4410925 pairs aligned concordantly 0 times; of these: 337822 (7.66%) aligned discordantly 1 time ---- 4073103 pairs aligned 0 times concordantly or discordantly; of these: 8146206 mates make up the pairs; of these: 4606634 (56.55%) aligned 0 times 2998650 (36.81%) aligned exactly 1 time 540922 (6.64%) aligned >1 times 89.09% overall alignment rate Time searching: 00:16:56 Overall time: 00:16:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4750327 / 17044854 = 0.2787 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:03:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:03:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:03:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:03:46: 1000000 INFO @ Sat, 15 Jan 2022 20:03:54: 2000000 INFO @ Sat, 15 Jan 2022 20:04:01: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:04:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:04:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:04:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:04:08: 4000000 INFO @ Sat, 15 Jan 2022 20:04:16: 1000000 INFO @ Sat, 15 Jan 2022 20:04:17: 5000000 INFO @ Sat, 15 Jan 2022 20:04:23: 2000000 INFO @ Sat, 15 Jan 2022 20:04:24: 6000000 INFO @ Sat, 15 Jan 2022 20:04:30: 3000000 INFO @ Sat, 15 Jan 2022 20:04:32: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:04:38: 4000000 INFO @ Sat, 15 Jan 2022 20:04:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:04:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:04:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:04:40: 8000000 INFO @ Sat, 15 Jan 2022 20:04:46: 5000000 INFO @ Sat, 15 Jan 2022 20:04:46: 1000000 INFO @ Sat, 15 Jan 2022 20:04:48: 9000000 INFO @ Sat, 15 Jan 2022 20:04:53: 6000000 INFO @ Sat, 15 Jan 2022 20:04:54: 2000000 INFO @ Sat, 15 Jan 2022 20:04:56: 10000000 INFO @ Sat, 15 Jan 2022 20:05:01: 7000000 INFO @ Sat, 15 Jan 2022 20:05:02: 3000000 INFO @ Sat, 15 Jan 2022 20:05:04: 11000000 INFO @ Sat, 15 Jan 2022 20:05:09: 8000000 INFO @ Sat, 15 Jan 2022 20:05:10: 4000000 INFO @ Sat, 15 Jan 2022 20:05:12: 12000000 INFO @ Sat, 15 Jan 2022 20:05:17: 9000000 INFO @ Sat, 15 Jan 2022 20:05:18: 5000000 INFO @ Sat, 15 Jan 2022 20:05:21: 13000000 INFO @ Sat, 15 Jan 2022 20:05:25: 10000000 INFO @ Sat, 15 Jan 2022 20:05:25: 6000000 INFO @ Sat, 15 Jan 2022 20:05:28: 14000000 INFO @ Sat, 15 Jan 2022 20:05:33: 11000000 INFO @ Sat, 15 Jan 2022 20:05:33: 7000000 INFO @ Sat, 15 Jan 2022 20:05:36: 15000000 INFO @ Sat, 15 Jan 2022 20:05:40: 12000000 INFO @ Sat, 15 Jan 2022 20:05:41: 8000000 INFO @ Sat, 15 Jan 2022 20:05:45: 16000000 INFO @ Sat, 15 Jan 2022 20:05:48: 13000000 INFO @ Sat, 15 Jan 2022 20:05:49: 9000000 INFO @ Sat, 15 Jan 2022 20:05:53: 17000000 INFO @ Sat, 15 Jan 2022 20:05:56: 10000000 INFO @ Sat, 15 Jan 2022 20:05:56: 14000000 INFO @ Sat, 15 Jan 2022 20:06:01: 18000000 INFO @ Sat, 15 Jan 2022 20:06:03: 11000000 INFO @ Sat, 15 Jan 2022 20:06:04: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:06:09: 19000000 INFO @ Sat, 15 Jan 2022 20:06:11: 12000000 INFO @ Sat, 15 Jan 2022 20:06:12: 16000000 INFO @ Sat, 15 Jan 2022 20:06:17: 20000000 INFO @ Sat, 15 Jan 2022 20:06:19: 13000000 INFO @ Sat, 15 Jan 2022 20:06:19: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:06:25: 21000000 INFO @ Sat, 15 Jan 2022 20:06:27: 14000000 INFO @ Sat, 15 Jan 2022 20:06:27: 18000000 INFO @ Sat, 15 Jan 2022 20:06:33: 22000000 INFO @ Sat, 15 Jan 2022 20:06:35: 15000000 INFO @ Sat, 15 Jan 2022 20:06:35: 19000000 INFO @ Sat, 15 Jan 2022 20:06:42: 23000000 INFO @ Sat, 15 Jan 2022 20:06:43: 16000000 INFO @ Sat, 15 Jan 2022 20:06:43: 20000000 INFO @ Sat, 15 Jan 2022 20:06:50: 24000000 INFO @ Sat, 15 Jan 2022 20:06:51: 17000000 INFO @ Sat, 15 Jan 2022 20:06:51: 21000000 INFO @ Sat, 15 Jan 2022 20:06:58: 25000000 INFO @ Sat, 15 Jan 2022 20:06:58: 18000000 INFO @ Sat, 15 Jan 2022 20:06:59: 22000000 INFO @ Sat, 15 Jan 2022 20:07:06: 26000000 INFO @ Sat, 15 Jan 2022 20:07:06: 19000000 INFO @ Sat, 15 Jan 2022 20:07:07: 23000000 INFO @ Sat, 15 Jan 2022 20:07:14: 27000000 INFO @ Sat, 15 Jan 2022 20:07:14: 20000000 INFO @ Sat, 15 Jan 2022 20:07:15: 24000000 INFO @ Sat, 15 Jan 2022 20:07:22: 21000000 INFO @ Sat, 15 Jan 2022 20:07:22: 28000000 INFO @ Sat, 15 Jan 2022 20:07:22: 25000000 INFO @ Sat, 15 Jan 2022 20:07:23: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:07:23: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:07:23: #1 total tags in treatment: 12011223 INFO @ Sat, 15 Jan 2022 20:07:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:07:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:07:23: #1 tags after filtering in treatment: 6454468 INFO @ Sat, 15 Jan 2022 20:07:23: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 20:07:23: #1 finished! INFO @ Sat, 15 Jan 2022 20:07:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:07:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:07:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:07:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:07:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:07:29: 22000000 INFO @ Sat, 15 Jan 2022 20:07:30: 26000000 INFO @ Sat, 15 Jan 2022 20:07:36: 23000000 INFO @ Sat, 15 Jan 2022 20:07:37: 27000000 INFO @ Sat, 15 Jan 2022 20:07:43: 24000000 INFO @ Sat, 15 Jan 2022 20:07:44: 28000000 INFO @ Sat, 15 Jan 2022 20:07:45: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:07:45: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:07:45: #1 total tags in treatment: 12011223 INFO @ Sat, 15 Jan 2022 20:07:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:07:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:07:45: #1 tags after filtering in treatment: 6454468 INFO @ Sat, 15 Jan 2022 20:07:45: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 20:07:45: #1 finished! INFO @ Sat, 15 Jan 2022 20:07:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:07:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:07:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:07:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:07:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:07:50: 25000000 INFO @ Sat, 15 Jan 2022 20:07:57: 26000000 INFO @ Sat, 15 Jan 2022 20:08:03: 27000000 INFO @ Sat, 15 Jan 2022 20:08:10: 28000000 INFO @ Sat, 15 Jan 2022 20:08:11: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:08:11: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:08:11: #1 total tags in treatment: 12011223 INFO @ Sat, 15 Jan 2022 20:08:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:08:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:08:11: #1 tags after filtering in treatment: 6454468 INFO @ Sat, 15 Jan 2022 20:08:11: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 20:08:11: #1 finished! INFO @ Sat, 15 Jan 2022 20:08:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:08:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:08:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:08:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:08:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781097/SRX11781097.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling