Job ID = 14520639 SRX = SRX11781096 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:29:32 prefetch.2.10.7: 1) Downloading 'SRR15481039'... 2022-01-15T10:29:32 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:31:02 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:31:02 prefetch.2.10.7: 1) 'SRR15481039' was downloaded successfully 2022-01-15T10:31:02 prefetch.2.10.7: 'SRR15481039' has 0 unresolved dependencies Read 22654027 spots for SRR15481039/SRR15481039.sra Written 22654027 spots for SRR15481039/SRR15481039.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:55:39 22654027 reads; of these: 22654027 (100.00%) were paired; of these: 5079200 (22.42%) aligned concordantly 0 times 10314671 (45.53%) aligned concordantly exactly 1 time 7260156 (32.05%) aligned concordantly >1 times ---- 5079200 pairs aligned concordantly 0 times; of these: 132205 (2.60%) aligned discordantly 1 time ---- 4946995 pairs aligned 0 times concordantly or discordantly; of these: 9893990 mates make up the pairs; of these: 6734741 (68.07%) aligned 0 times 1797025 (18.16%) aligned exactly 1 time 1362224 (13.77%) aligned >1 times 85.14% overall alignment rate Time searching: 00:55:39 Overall time: 00:55:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3367340 / 17704430 = 0.1902 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:47:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:47:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:47:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:47:33: 1000000 INFO @ Sat, 15 Jan 2022 20:47:42: 2000000 INFO @ Sat, 15 Jan 2022 20:47:51: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:47:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:47:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:47:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:48:00: 4000000 INFO @ Sat, 15 Jan 2022 20:48:01: 1000000 INFO @ Sat, 15 Jan 2022 20:48:08: 5000000 INFO @ Sat, 15 Jan 2022 20:48:08: 2000000 INFO @ Sat, 15 Jan 2022 20:48:16: 3000000 INFO @ Sat, 15 Jan 2022 20:48:16: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:48:23: 4000000 INFO @ Sat, 15 Jan 2022 20:48:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:48:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:48:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:48:24: 7000000 INFO @ Sat, 15 Jan 2022 20:48:30: 5000000 INFO @ Sat, 15 Jan 2022 20:48:32: 1000000 INFO @ Sat, 15 Jan 2022 20:48:33: 8000000 INFO @ Sat, 15 Jan 2022 20:48:38: 6000000 INFO @ Sat, 15 Jan 2022 20:48:40: 2000000 INFO @ Sat, 15 Jan 2022 20:48:41: 9000000 INFO @ Sat, 15 Jan 2022 20:48:45: 7000000 INFO @ Sat, 15 Jan 2022 20:48:49: 3000000 INFO @ Sat, 15 Jan 2022 20:48:49: 10000000 INFO @ Sat, 15 Jan 2022 20:48:53: 8000000 INFO @ Sat, 15 Jan 2022 20:48:56: 4000000 INFO @ Sat, 15 Jan 2022 20:48:57: 11000000 INFO @ Sat, 15 Jan 2022 20:49:00: 9000000 INFO @ Sat, 15 Jan 2022 20:49:03: 5000000 INFO @ Sat, 15 Jan 2022 20:49:04: 12000000 INFO @ Sat, 15 Jan 2022 20:49:07: 10000000 INFO @ Sat, 15 Jan 2022 20:49:11: 6000000 INFO @ Sat, 15 Jan 2022 20:49:11: 13000000 INFO @ Sat, 15 Jan 2022 20:49:15: 11000000 INFO @ Sat, 15 Jan 2022 20:49:19: 7000000 INFO @ Sat, 15 Jan 2022 20:49:19: 14000000 INFO @ Sat, 15 Jan 2022 20:49:22: 12000000 INFO @ Sat, 15 Jan 2022 20:49:27: 8000000 INFO @ Sat, 15 Jan 2022 20:49:27: 15000000 INFO @ Sat, 15 Jan 2022 20:49:31: 13000000 INFO @ Sat, 15 Jan 2022 20:49:34: 9000000 INFO @ Sat, 15 Jan 2022 20:49:34: 16000000 INFO @ Sat, 15 Jan 2022 20:49:38: 14000000 INFO @ Sat, 15 Jan 2022 20:49:42: 10000000 INFO @ Sat, 15 Jan 2022 20:49:42: 17000000 INFO @ Sat, 15 Jan 2022 20:49:46: 15000000 INFO @ Sat, 15 Jan 2022 20:49:49: 11000000 INFO @ Sat, 15 Jan 2022 20:49:49: 18000000 INFO @ Sat, 15 Jan 2022 20:49:53: 16000000 INFO @ Sat, 15 Jan 2022 20:49:57: 19000000 INFO @ Sat, 15 Jan 2022 20:49:57: 12000000 INFO @ Sat, 15 Jan 2022 20:50:00: 17000000 INFO @ Sat, 15 Jan 2022 20:50:04: 20000000 INFO @ Sat, 15 Jan 2022 20:50:05: 13000000 INFO @ Sat, 15 Jan 2022 20:50:07: 18000000 INFO @ Sat, 15 Jan 2022 20:50:11: 21000000 INFO @ Sat, 15 Jan 2022 20:50:12: 14000000 INFO @ Sat, 15 Jan 2022 20:50:14: 19000000 INFO @ Sat, 15 Jan 2022 20:50:19: 22000000 INFO @ Sat, 15 Jan 2022 20:50:20: 15000000 INFO @ Sat, 15 Jan 2022 20:50:21: 20000000 INFO @ Sat, 15 Jan 2022 20:50:27: 23000000 INFO @ Sat, 15 Jan 2022 20:50:27: 21000000 INFO @ Sat, 15 Jan 2022 20:50:29: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:50:34: 22000000 INFO @ Sat, 15 Jan 2022 20:50:36: 24000000 INFO @ Sat, 15 Jan 2022 20:50:38: 17000000 INFO @ Sat, 15 Jan 2022 20:50:41: 23000000 INFO @ Sat, 15 Jan 2022 20:50:44: 25000000 INFO @ Sat, 15 Jan 2022 20:50:47: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:50:49: 24000000 INFO @ Sat, 15 Jan 2022 20:50:53: 26000000 INFO @ Sat, 15 Jan 2022 20:50:56: 19000000 INFO @ Sat, 15 Jan 2022 20:50:57: 25000000 INFO @ Sat, 15 Jan 2022 20:51:02: 27000000 INFO @ Sat, 15 Jan 2022 20:51:05: 20000000 INFO @ Sat, 15 Jan 2022 20:51:06: 26000000 INFO @ Sat, 15 Jan 2022 20:51:10: 28000000 INFO @ Sat, 15 Jan 2022 20:51:13: 21000000 INFO @ Sat, 15 Jan 2022 20:51:14: 27000000 INFO @ Sat, 15 Jan 2022 20:51:19: 29000000 INFO @ Sat, 15 Jan 2022 20:51:22: 22000000 INFO @ Sat, 15 Jan 2022 20:51:23: 28000000 INFO @ Sat, 15 Jan 2022 20:51:28: 30000000 INFO @ Sat, 15 Jan 2022 20:51:31: 23000000 INFO @ Sat, 15 Jan 2022 20:51:33: 29000000 INFO @ Sat, 15 Jan 2022 20:51:37: 31000000 INFO @ Sat, 15 Jan 2022 20:51:41: 24000000 INFO @ Sat, 15 Jan 2022 20:51:42: 30000000 INFO @ Sat, 15 Jan 2022 20:51:44: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:51:44: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:51:44: #1 total tags in treatment: 14218450 INFO @ Sat, 15 Jan 2022 20:51:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:51:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:51:44: #1 tags after filtering in treatment: 6580147 INFO @ Sat, 15 Jan 2022 20:51:44: #1 Redundant rate of treatment: 0.54 INFO @ Sat, 15 Jan 2022 20:51:44: #1 finished! INFO @ Sat, 15 Jan 2022 20:51:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:51:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:51:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:51:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:51:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:51:49: 25000000 INFO @ Sat, 15 Jan 2022 20:51:51: 31000000 INFO @ Sat, 15 Jan 2022 20:51:58: 26000000 INFO @ Sat, 15 Jan 2022 20:51:58: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:51:58: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:51:58: #1 total tags in treatment: 14218450 INFO @ Sat, 15 Jan 2022 20:51:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:51:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:51:59: #1 tags after filtering in treatment: 6580147 INFO @ Sat, 15 Jan 2022 20:51:59: #1 Redundant rate of treatment: 0.54 INFO @ Sat, 15 Jan 2022 20:51:59: #1 finished! INFO @ Sat, 15 Jan 2022 20:51:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:51:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:51:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:51:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:51:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:52:05: 27000000 INFO @ Sat, 15 Jan 2022 20:52:12: 28000000 INFO @ Sat, 15 Jan 2022 20:52:18: 29000000 INFO @ Sat, 15 Jan 2022 20:52:25: 30000000 INFO @ Sat, 15 Jan 2022 20:52:32: 31000000 INFO @ Sat, 15 Jan 2022 20:52:37: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:52:37: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:52:37: #1 total tags in treatment: 14218450 INFO @ Sat, 15 Jan 2022 20:52:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:52:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:52:38: #1 tags after filtering in treatment: 6580147 INFO @ Sat, 15 Jan 2022 20:52:38: #1 Redundant rate of treatment: 0.54 INFO @ Sat, 15 Jan 2022 20:52:38: #1 finished! INFO @ Sat, 15 Jan 2022 20:52:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:52:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:52:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:52:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:52:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781096/SRX11781096.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling