Job ID = 14520638 SRX = SRX11781095 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:29:32 prefetch.2.10.7: 1) Downloading 'SRR15481038'... 2022-01-15T10:29:32 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:32:52 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:32:52 prefetch.2.10.7: 1) 'SRR15481038' was downloaded successfully 2022-01-15T10:32:52 prefetch.2.10.7: 'SRR15481038' has 0 unresolved dependencies Read 20977076 spots for SRR15481038/SRR15481038.sra Written 20977076 spots for SRR15481038/SRR15481038.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:39 20977076 reads; of these: 20977076 (100.00%) were paired; of these: 3837415 (18.29%) aligned concordantly 0 times 15419320 (73.51%) aligned concordantly exactly 1 time 1720341 (8.20%) aligned concordantly >1 times ---- 3837415 pairs aligned concordantly 0 times; of these: 602433 (15.70%) aligned discordantly 1 time ---- 3234982 pairs aligned 0 times concordantly or discordantly; of these: 6469964 mates make up the pairs; of these: 4008361 (61.95%) aligned 0 times 2071005 (32.01%) aligned exactly 1 time 390598 (6.04%) aligned >1 times 90.45% overall alignment rate Time searching: 00:16:39 Overall time: 00:16:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4762683 / 17740446 = 0.2685 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:06:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:06:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:06:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:06:48: 1000000 INFO @ Sat, 15 Jan 2022 20:06:53: 2000000 INFO @ Sat, 15 Jan 2022 20:06:58: 3000000 INFO @ Sat, 15 Jan 2022 20:07:02: 4000000 INFO @ Sat, 15 Jan 2022 20:07:07: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:07:12: 6000000 INFO @ Sat, 15 Jan 2022 20:07:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:07:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:07:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:07:16: 7000000 INFO @ Sat, 15 Jan 2022 20:07:18: 1000000 INFO @ Sat, 15 Jan 2022 20:07:21: 8000000 INFO @ Sat, 15 Jan 2022 20:07:23: 2000000 INFO @ Sat, 15 Jan 2022 20:07:25: 9000000 INFO @ Sat, 15 Jan 2022 20:07:28: 3000000 INFO @ Sat, 15 Jan 2022 20:07:30: 10000000 INFO @ Sat, 15 Jan 2022 20:07:33: 4000000 INFO @ Sat, 15 Jan 2022 20:07:35: 11000000 INFO @ Sat, 15 Jan 2022 20:07:37: 5000000 INFO @ Sat, 15 Jan 2022 20:07:39: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:07:42: 6000000 INFO @ Sat, 15 Jan 2022 20:07:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:07:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:07:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:07:44: 13000000 INFO @ Sat, 15 Jan 2022 20:07:47: 7000000 INFO @ Sat, 15 Jan 2022 20:07:48: 1000000 INFO @ Sat, 15 Jan 2022 20:07:49: 14000000 INFO @ Sat, 15 Jan 2022 20:07:51: 8000000 INFO @ Sat, 15 Jan 2022 20:07:53: 2000000 INFO @ Sat, 15 Jan 2022 20:07:53: 15000000 INFO @ Sat, 15 Jan 2022 20:07:56: 9000000 INFO @ Sat, 15 Jan 2022 20:07:58: 3000000 INFO @ Sat, 15 Jan 2022 20:07:58: 16000000 INFO @ Sat, 15 Jan 2022 20:08:00: 10000000 INFO @ Sat, 15 Jan 2022 20:08:03: 4000000 INFO @ Sat, 15 Jan 2022 20:08:03: 17000000 INFO @ Sat, 15 Jan 2022 20:08:05: 11000000 INFO @ Sat, 15 Jan 2022 20:08:08: 18000000 INFO @ Sat, 15 Jan 2022 20:08:08: 5000000 INFO @ Sat, 15 Jan 2022 20:08:10: 12000000 INFO @ Sat, 15 Jan 2022 20:08:12: 19000000 INFO @ Sat, 15 Jan 2022 20:08:13: 6000000 INFO @ Sat, 15 Jan 2022 20:08:14: 13000000 INFO @ Sat, 15 Jan 2022 20:08:17: 7000000 INFO @ Sat, 15 Jan 2022 20:08:17: 20000000 INFO @ Sat, 15 Jan 2022 20:08:19: 14000000 INFO @ Sat, 15 Jan 2022 20:08:22: 8000000 INFO @ Sat, 15 Jan 2022 20:08:22: 21000000 INFO @ Sat, 15 Jan 2022 20:08:24: 15000000 INFO @ Sat, 15 Jan 2022 20:08:27: 9000000 INFO @ Sat, 15 Jan 2022 20:08:27: 22000000 INFO @ Sat, 15 Jan 2022 20:08:28: 16000000 INFO @ Sat, 15 Jan 2022 20:08:31: 10000000 INFO @ Sat, 15 Jan 2022 20:08:31: 23000000 INFO @ Sat, 15 Jan 2022 20:08:33: 17000000 INFO @ Sat, 15 Jan 2022 20:08:36: 11000000 INFO @ Sat, 15 Jan 2022 20:08:36: 24000000 INFO @ Sat, 15 Jan 2022 20:08:38: 18000000 INFO @ Sat, 15 Jan 2022 20:08:41: 12000000 INFO @ Sat, 15 Jan 2022 20:08:41: 25000000 INFO @ Sat, 15 Jan 2022 20:08:43: 19000000 INFO @ Sat, 15 Jan 2022 20:08:45: 13000000 INFO @ Sat, 15 Jan 2022 20:08:46: 26000000 INFO @ Sat, 15 Jan 2022 20:08:48: 20000000 INFO @ Sat, 15 Jan 2022 20:08:50: 14000000 INFO @ Sat, 15 Jan 2022 20:08:51: 27000000 INFO @ Sat, 15 Jan 2022 20:08:52: 21000000 INFO @ Sat, 15 Jan 2022 20:08:55: 15000000 INFO @ Sat, 15 Jan 2022 20:08:55: 28000000 INFO @ Sat, 15 Jan 2022 20:08:57: 22000000 INFO @ Sat, 15 Jan 2022 20:08:58: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:08:58: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:08:58: #1 total tags in treatment: 12474759 INFO @ Sat, 15 Jan 2022 20:08:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:08:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:08:58: #1 tags after filtering in treatment: 6787818 INFO @ Sat, 15 Jan 2022 20:08:58: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 20:08:58: #1 finished! INFO @ Sat, 15 Jan 2022 20:08:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:08:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:08:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:08:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:08:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:09:00: 16000000 INFO @ Sat, 15 Jan 2022 20:09:02: 23000000 INFO @ Sat, 15 Jan 2022 20:09:04: 17000000 INFO @ Sat, 15 Jan 2022 20:09:07: 24000000 INFO @ Sat, 15 Jan 2022 20:09:09: 18000000 INFO @ Sat, 15 Jan 2022 20:09:11: 25000000 INFO @ Sat, 15 Jan 2022 20:09:14: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:09:16: 26000000 INFO @ Sat, 15 Jan 2022 20:09:19: 20000000 INFO @ Sat, 15 Jan 2022 20:09:21: 27000000 INFO @ Sat, 15 Jan 2022 20:09:23: 21000000 INFO @ Sat, 15 Jan 2022 20:09:26: 28000000 INFO @ Sat, 15 Jan 2022 20:09:28: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:09:28: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:09:28: #1 total tags in treatment: 12474759 INFO @ Sat, 15 Jan 2022 20:09:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:28: #1 tags after filtering in treatment: 6787818 INFO @ Sat, 15 Jan 2022 20:09:28: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 20:09:28: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:28: 22000000 INFO @ Sat, 15 Jan 2022 20:09:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:09:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:09:33: 23000000 INFO @ Sat, 15 Jan 2022 20:09:38: 24000000 INFO @ Sat, 15 Jan 2022 20:09:43: 25000000 INFO @ Sat, 15 Jan 2022 20:09:47: 26000000 INFO @ Sat, 15 Jan 2022 20:09:52: 27000000 INFO @ Sat, 15 Jan 2022 20:09:57: 28000000 INFO @ Sat, 15 Jan 2022 20:09:59: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:09:59: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:09:59: #1 total tags in treatment: 12474759 INFO @ Sat, 15 Jan 2022 20:09:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:59: #1 tags after filtering in treatment: 6787818 INFO @ Sat, 15 Jan 2022 20:09:59: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 20:09:59: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:09:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781095/SRX11781095.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling