Job ID = 14520636 SRX = SRX11781093 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:29:17 prefetch.2.10.7: 1) Downloading 'SRR15481036'... 2022-01-15T10:29:17 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:30:32 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:30:32 prefetch.2.10.7: 1) 'SRR15481036' was downloaded successfully 2022-01-15T10:30:32 prefetch.2.10.7: 'SRR15481036' has 0 unresolved dependencies Read 17266703 spots for SRR15481036/SRR15481036.sra Written 17266703 spots for SRR15481036/SRR15481036.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:00 17266703 reads; of these: 17266703 (100.00%) were paired; of these: 2956614 (17.12%) aligned concordantly 0 times 13096443 (75.85%) aligned concordantly exactly 1 time 1213646 (7.03%) aligned concordantly >1 times ---- 2956614 pairs aligned concordantly 0 times; of these: 261328 (8.84%) aligned discordantly 1 time ---- 2695286 pairs aligned 0 times concordantly or discordantly; of these: 5390572 mates make up the pairs; of these: 3434492 (63.71%) aligned 0 times 1710932 (31.74%) aligned exactly 1 time 245148 (4.55%) aligned >1 times 90.05% overall alignment rate Time searching: 00:14:00 Overall time: 00:14:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4317635 / 14570332 = 0.2963 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:05:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:05:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:05:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:05:24: 1000000 INFO @ Sat, 15 Jan 2022 20:05:31: 2000000 INFO @ Sat, 15 Jan 2022 20:05:38: 3000000 INFO @ Sat, 15 Jan 2022 20:05:45: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:05:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:05:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:05:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:05:52: 5000000 INFO @ Sat, 15 Jan 2022 20:05:54: 1000000 INFO @ Sat, 15 Jan 2022 20:05:59: 6000000 INFO @ Sat, 15 Jan 2022 20:06:02: 2000000 INFO @ Sat, 15 Jan 2022 20:06:07: 7000000 INFO @ Sat, 15 Jan 2022 20:06:09: 3000000 INFO @ Sat, 15 Jan 2022 20:06:14: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:06:16: 4000000 INFO @ Sat, 15 Jan 2022 20:06:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:06:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:06:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:06:21: 9000000 INFO @ Sat, 15 Jan 2022 20:06:23: 1000000 INFO @ Sat, 15 Jan 2022 20:06:24: 5000000 INFO @ Sat, 15 Jan 2022 20:06:29: 10000000 INFO @ Sat, 15 Jan 2022 20:06:30: 2000000 INFO @ Sat, 15 Jan 2022 20:06:32: 6000000 INFO @ Sat, 15 Jan 2022 20:06:37: 11000000 INFO @ Sat, 15 Jan 2022 20:06:37: 3000000 INFO @ Sat, 15 Jan 2022 20:06:40: 7000000 INFO @ Sat, 15 Jan 2022 20:06:44: 4000000 INFO @ Sat, 15 Jan 2022 20:06:45: 12000000 INFO @ Sat, 15 Jan 2022 20:06:47: 8000000 INFO @ Sat, 15 Jan 2022 20:06:50: 5000000 INFO @ Sat, 15 Jan 2022 20:06:52: 13000000 INFO @ Sat, 15 Jan 2022 20:06:55: 9000000 INFO @ Sat, 15 Jan 2022 20:06:57: 6000000 INFO @ Sat, 15 Jan 2022 20:07:00: 14000000 INFO @ Sat, 15 Jan 2022 20:07:03: 10000000 INFO @ Sat, 15 Jan 2022 20:07:04: 7000000 INFO @ Sat, 15 Jan 2022 20:07:08: 15000000 INFO @ Sat, 15 Jan 2022 20:07:10: 8000000 INFO @ Sat, 15 Jan 2022 20:07:11: 11000000 INFO @ Sat, 15 Jan 2022 20:07:16: 16000000 INFO @ Sat, 15 Jan 2022 20:07:17: 9000000 INFO @ Sat, 15 Jan 2022 20:07:19: 12000000 INFO @ Sat, 15 Jan 2022 20:07:23: 10000000 INFO @ Sat, 15 Jan 2022 20:07:24: 17000000 INFO @ Sat, 15 Jan 2022 20:07:26: 13000000 INFO @ Sat, 15 Jan 2022 20:07:30: 11000000 INFO @ Sat, 15 Jan 2022 20:07:32: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:07:34: 14000000 INFO @ Sat, 15 Jan 2022 20:07:37: 12000000 INFO @ Sat, 15 Jan 2022 20:07:40: 19000000 INFO @ Sat, 15 Jan 2022 20:07:42: 15000000 INFO @ Sat, 15 Jan 2022 20:07:43: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:07:48: 20000000 INFO @ Sat, 15 Jan 2022 20:07:50: 16000000 INFO @ Sat, 15 Jan 2022 20:07:50: 14000000 INFO @ Sat, 15 Jan 2022 20:07:55: 21000000 INFO @ Sat, 15 Jan 2022 20:07:57: 15000000 INFO @ Sat, 15 Jan 2022 20:07:58: 17000000 INFO @ Sat, 15 Jan 2022 20:08:03: 22000000 INFO @ Sat, 15 Jan 2022 20:08:04: 16000000 INFO @ Sat, 15 Jan 2022 20:08:06: 18000000 INFO @ Sat, 15 Jan 2022 20:08:07: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:08:07: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:08:07: #1 total tags in treatment: 10040537 INFO @ Sat, 15 Jan 2022 20:08:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:08:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:08:07: #1 tags after filtering in treatment: 5091384 INFO @ Sat, 15 Jan 2022 20:08:07: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 20:08:07: #1 finished! INFO @ Sat, 15 Jan 2022 20:08:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:08:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:08:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:08:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:08:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:08:11: 17000000 INFO @ Sat, 15 Jan 2022 20:08:13: 19000000 INFO @ Sat, 15 Jan 2022 20:08:17: 18000000 INFO @ Sat, 15 Jan 2022 20:08:21: 20000000 INFO @ Sat, 15 Jan 2022 20:08:24: 19000000 INFO @ Sat, 15 Jan 2022 20:08:29: 21000000 INFO @ Sat, 15 Jan 2022 20:08:31: 20000000 INFO @ Sat, 15 Jan 2022 20:08:36: 22000000 INFO @ Sat, 15 Jan 2022 20:08:38: 21000000 INFO @ Sat, 15 Jan 2022 20:08:39: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:08:39: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:08:39: #1 total tags in treatment: 10040537 INFO @ Sat, 15 Jan 2022 20:08:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:08:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:08:40: #1 tags after filtering in treatment: 5091384 INFO @ Sat, 15 Jan 2022 20:08:40: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 20:08:40: #1 finished! INFO @ Sat, 15 Jan 2022 20:08:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:08:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:08:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:08:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:08:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:08:44: 22000000 INFO @ Sat, 15 Jan 2022 20:08:47: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:08:47: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:08:47: #1 total tags in treatment: 10040537 INFO @ Sat, 15 Jan 2022 20:08:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:08:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:08:47: #1 tags after filtering in treatment: 5091384 INFO @ Sat, 15 Jan 2022 20:08:47: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 20:08:47: #1 finished! INFO @ Sat, 15 Jan 2022 20:08:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:08:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:08:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:08:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:08:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781093/SRX11781093.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling