Job ID = 14520634 SRX = SRX11781091 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:29:06 prefetch.2.10.7: 1) Downloading 'SRR15481034'... 2022-01-15T10:29:06 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:31:01 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:31:01 prefetch.2.10.7: 1) 'SRR15481034' was downloaded successfully 2022-01-15T10:31:01 prefetch.2.10.7: 'SRR15481034' has 0 unresolved dependencies Read 21831298 spots for SRR15481034/SRR15481034.sra Written 21831298 spots for SRR15481034/SRR15481034.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:45 21831298 reads; of these: 21831298 (100.00%) were paired; of these: 5253841 (24.07%) aligned concordantly 0 times 14657109 (67.14%) aligned concordantly exactly 1 time 1920348 (8.80%) aligned concordantly >1 times ---- 5253841 pairs aligned concordantly 0 times; of these: 335832 (6.39%) aligned discordantly 1 time ---- 4918009 pairs aligned 0 times concordantly or discordantly; of these: 9836018 mates make up the pairs; of these: 5736253 (58.32%) aligned 0 times 3507390 (35.66%) aligned exactly 1 time 592375 (6.02%) aligned >1 times 86.86% overall alignment rate Time searching: 00:23:45 Overall time: 00:23:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3368720 / 16911822 = 0.1992 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:13:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:13:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:13:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:13:09: 1000000 INFO @ Sat, 15 Jan 2022 20:13:16: 2000000 INFO @ Sat, 15 Jan 2022 20:13:22: 3000000 INFO @ Sat, 15 Jan 2022 20:13:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:13:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:13:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:13:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:13:36: 5000000 INFO @ Sat, 15 Jan 2022 20:13:40: 1000000 INFO @ Sat, 15 Jan 2022 20:13:44: 6000000 INFO @ Sat, 15 Jan 2022 20:13:47: 2000000 INFO @ Sat, 15 Jan 2022 20:13:51: 7000000 INFO @ Sat, 15 Jan 2022 20:13:54: 3000000 INFO @ Sat, 15 Jan 2022 20:13:58: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:14:01: 4000000 INFO @ Sat, 15 Jan 2022 20:14:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:14:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:14:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:14:06: 9000000 INFO @ Sat, 15 Jan 2022 20:14:09: 5000000 INFO @ Sat, 15 Jan 2022 20:14:11: 1000000 INFO @ Sat, 15 Jan 2022 20:14:14: 10000000 INFO @ Sat, 15 Jan 2022 20:14:17: 6000000 INFO @ Sat, 15 Jan 2022 20:14:19: 2000000 INFO @ Sat, 15 Jan 2022 20:14:22: 11000000 INFO @ Sat, 15 Jan 2022 20:14:25: 7000000 INFO @ Sat, 15 Jan 2022 20:14:27: 3000000 INFO @ Sat, 15 Jan 2022 20:14:30: 12000000 INFO @ Sat, 15 Jan 2022 20:14:33: 8000000 INFO @ Sat, 15 Jan 2022 20:14:35: 4000000 INFO @ Sat, 15 Jan 2022 20:14:38: 13000000 INFO @ Sat, 15 Jan 2022 20:14:41: 9000000 INFO @ Sat, 15 Jan 2022 20:14:44: 5000000 INFO @ Sat, 15 Jan 2022 20:14:46: 14000000 INFO @ Sat, 15 Jan 2022 20:14:49: 10000000 INFO @ Sat, 15 Jan 2022 20:14:52: 6000000 INFO @ Sat, 15 Jan 2022 20:14:54: 15000000 INFO @ Sat, 15 Jan 2022 20:14:57: 11000000 INFO @ Sat, 15 Jan 2022 20:15:00: 7000000 INFO @ Sat, 15 Jan 2022 20:15:02: 16000000 INFO @ Sat, 15 Jan 2022 20:15:05: 12000000 INFO @ Sat, 15 Jan 2022 20:15:08: 8000000 INFO @ Sat, 15 Jan 2022 20:15:10: 17000000 INFO @ Sat, 15 Jan 2022 20:15:13: 13000000 INFO @ Sat, 15 Jan 2022 20:15:16: 9000000 INFO @ Sat, 15 Jan 2022 20:15:19: 18000000 INFO @ Sat, 15 Jan 2022 20:15:21: 14000000 INFO @ Sat, 15 Jan 2022 20:15:24: 10000000 INFO @ Sat, 15 Jan 2022 20:15:27: 19000000 INFO @ Sat, 15 Jan 2022 20:15:30: 15000000 INFO @ Sat, 15 Jan 2022 20:15:32: 11000000 INFO @ Sat, 15 Jan 2022 20:15:35: 20000000 INFO @ Sat, 15 Jan 2022 20:15:38: 16000000 INFO @ Sat, 15 Jan 2022 20:15:40: 12000000 INFO @ Sat, 15 Jan 2022 20:15:43: 21000000 INFO @ Sat, 15 Jan 2022 20:15:46: 17000000 INFO @ Sat, 15 Jan 2022 20:15:48: 13000000 INFO @ Sat, 15 Jan 2022 20:15:51: 22000000 INFO @ Sat, 15 Jan 2022 20:15:54: 18000000 INFO @ Sat, 15 Jan 2022 20:15:56: 14000000 INFO @ Sat, 15 Jan 2022 20:15:59: 23000000 INFO @ Sat, 15 Jan 2022 20:16:02: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:16:04: 15000000 INFO @ Sat, 15 Jan 2022 20:16:07: 24000000 INFO @ Sat, 15 Jan 2022 20:16:10: 20000000 INFO @ Sat, 15 Jan 2022 20:16:12: 16000000 INFO @ Sat, 15 Jan 2022 20:16:15: 25000000 INFO @ Sat, 15 Jan 2022 20:16:18: 21000000 INFO @ Sat, 15 Jan 2022 20:16:20: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:16:23: 26000000 INFO @ Sat, 15 Jan 2022 20:16:26: 22000000 INFO @ Sat, 15 Jan 2022 20:16:28: 18000000 INFO @ Sat, 15 Jan 2022 20:16:31: 27000000 INFO @ Sat, 15 Jan 2022 20:16:35: 23000000 INFO @ Sat, 15 Jan 2022 20:16:37: 19000000 INFO @ Sat, 15 Jan 2022 20:16:40: 28000000 INFO @ Sat, 15 Jan 2022 20:16:43: 24000000 INFO @ Sat, 15 Jan 2022 20:16:45: 20000000 INFO @ Sat, 15 Jan 2022 20:16:48: 29000000 INFO @ Sat, 15 Jan 2022 20:16:51: 25000000 INFO @ Sat, 15 Jan 2022 20:16:53: 21000000 INFO @ Sat, 15 Jan 2022 20:16:56: 30000000 INFO @ Sat, 15 Jan 2022 20:16:59: 26000000 INFO @ Sat, 15 Jan 2022 20:17:01: 22000000 INFO @ Sat, 15 Jan 2022 20:17:04: 31000000 INFO @ Sat, 15 Jan 2022 20:17:06: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:17:06: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:17:06: #1 total tags in treatment: 13247107 INFO @ Sat, 15 Jan 2022 20:17:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:06: #1 tags after filtering in treatment: 7423969 INFO @ Sat, 15 Jan 2022 20:17:06: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 20:17:06: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:17:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:17:07: 27000000 INFO @ Sat, 15 Jan 2022 20:17:09: 23000000 INFO @ Sat, 15 Jan 2022 20:17:14: 28000000 INFO @ Sat, 15 Jan 2022 20:17:16: 24000000 INFO @ Sat, 15 Jan 2022 20:17:22: 29000000 INFO @ Sat, 15 Jan 2022 20:17:24: 25000000 INFO @ Sat, 15 Jan 2022 20:17:29: 30000000 INFO @ Sat, 15 Jan 2022 20:17:31: 26000000 INFO @ Sat, 15 Jan 2022 20:17:37: 31000000 INFO @ Sat, 15 Jan 2022 20:17:38: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:17:38: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:17:38: #1 total tags in treatment: 13247107 INFO @ Sat, 15 Jan 2022 20:17:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:39: 27000000 INFO @ Sat, 15 Jan 2022 20:17:39: #1 tags after filtering in treatment: 7423969 INFO @ Sat, 15 Jan 2022 20:17:39: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 20:17:39: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:17:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:17:45: 28000000 INFO @ Sat, 15 Jan 2022 20:17:52: 29000000 INFO @ Sat, 15 Jan 2022 20:17:59: 30000000 INFO @ Sat, 15 Jan 2022 20:18:05: 31000000 INFO @ Sat, 15 Jan 2022 20:18:06: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:18:06: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:18:06: #1 total tags in treatment: 13247107 INFO @ Sat, 15 Jan 2022 20:18:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:18:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:18:07: #1 tags after filtering in treatment: 7423969 INFO @ Sat, 15 Jan 2022 20:18:07: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 20:18:07: #1 finished! INFO @ Sat, 15 Jan 2022 20:18:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:18:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:18:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:18:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:18:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781091/SRX11781091.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling