Job ID = 14520633 SRX = SRX11781090 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:28:47 prefetch.2.10.7: 1) Downloading 'SRR15481033'... 2022-01-15T10:28:47 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:30:49 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:30:49 prefetch.2.10.7: 1) 'SRR15481033' was downloaded successfully 2022-01-15T10:30:49 prefetch.2.10.7: 'SRR15481033' has 0 unresolved dependencies Read 17933086 spots for SRR15481033/SRR15481033.sra Written 17933086 spots for SRR15481033/SRR15481033.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:43:37 17933086 reads; of these: 17933086 (100.00%) were paired; of these: 3268824 (18.23%) aligned concordantly 0 times 6079306 (33.90%) aligned concordantly exactly 1 time 8584956 (47.87%) aligned concordantly >1 times ---- 3268824 pairs aligned concordantly 0 times; of these: 45391 (1.39%) aligned discordantly 1 time ---- 3223433 pairs aligned 0 times concordantly or discordantly; of these: 6446866 mates make up the pairs; of these: 4443131 (68.92%) aligned 0 times 807858 (12.53%) aligned exactly 1 time 1195877 (18.55%) aligned >1 times 87.61% overall alignment rate Time searching: 00:43:37 Overall time: 00:43:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2290977 / 14708258 = 0.1558 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:30: 1000000 INFO @ Sat, 15 Jan 2022 20:28:36: 2000000 INFO @ Sat, 15 Jan 2022 20:28:43: 3000000 INFO @ Sat, 15 Jan 2022 20:28:50: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:57: 5000000 INFO @ Sat, 15 Jan 2022 20:29:00: 1000000 INFO @ Sat, 15 Jan 2022 20:29:05: 6000000 INFO @ Sat, 15 Jan 2022 20:29:08: 2000000 INFO @ Sat, 15 Jan 2022 20:29:13: 7000000 INFO @ Sat, 15 Jan 2022 20:29:16: 3000000 INFO @ Sat, 15 Jan 2022 20:29:20: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:29:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:29:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:29:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:29:23: 4000000 INFO @ Sat, 15 Jan 2022 20:29:28: 9000000 INFO @ Sat, 15 Jan 2022 20:29:31: 1000000 INFO @ Sat, 15 Jan 2022 20:29:31: 5000000 INFO @ Sat, 15 Jan 2022 20:29:35: 10000000 INFO @ Sat, 15 Jan 2022 20:29:39: 2000000 INFO @ Sat, 15 Jan 2022 20:29:39: 6000000 INFO @ Sat, 15 Jan 2022 20:29:43: 11000000 INFO @ Sat, 15 Jan 2022 20:29:47: 3000000 INFO @ Sat, 15 Jan 2022 20:29:47: 7000000 INFO @ Sat, 15 Jan 2022 20:29:50: 12000000 INFO @ Sat, 15 Jan 2022 20:29:54: 4000000 INFO @ Sat, 15 Jan 2022 20:29:55: 8000000 INFO @ Sat, 15 Jan 2022 20:29:58: 13000000 INFO @ Sat, 15 Jan 2022 20:30:02: 5000000 INFO @ Sat, 15 Jan 2022 20:30:03: 9000000 INFO @ Sat, 15 Jan 2022 20:30:06: 14000000 INFO @ Sat, 15 Jan 2022 20:30:10: 6000000 INFO @ Sat, 15 Jan 2022 20:30:11: 10000000 INFO @ Sat, 15 Jan 2022 20:30:13: 15000000 INFO @ Sat, 15 Jan 2022 20:30:18: 7000000 INFO @ Sat, 15 Jan 2022 20:30:18: 11000000 INFO @ Sat, 15 Jan 2022 20:30:21: 16000000 INFO @ Sat, 15 Jan 2022 20:30:26: 8000000 INFO @ Sat, 15 Jan 2022 20:30:26: 12000000 INFO @ Sat, 15 Jan 2022 20:30:29: 17000000 INFO @ Sat, 15 Jan 2022 20:30:33: 9000000 INFO @ Sat, 15 Jan 2022 20:30:34: 13000000 INFO @ Sat, 15 Jan 2022 20:30:36: 18000000 INFO @ Sat, 15 Jan 2022 20:30:41: 10000000 INFO @ Sat, 15 Jan 2022 20:30:42: 14000000 INFO @ Sat, 15 Jan 2022 20:30:44: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:30:49: 11000000 INFO @ Sat, 15 Jan 2022 20:30:50: 15000000 INFO @ Sat, 15 Jan 2022 20:30:51: 20000000 INFO @ Sat, 15 Jan 2022 20:30:57: 12000000 INFO @ Sat, 15 Jan 2022 20:30:58: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:30:59: 21000000 INFO @ Sat, 15 Jan 2022 20:31:05: 13000000 INFO @ Sat, 15 Jan 2022 20:31:06: 17000000 INFO @ Sat, 15 Jan 2022 20:31:06: 22000000 INFO @ Sat, 15 Jan 2022 20:31:13: 14000000 INFO @ Sat, 15 Jan 2022 20:31:13: 18000000 INFO @ Sat, 15 Jan 2022 20:31:14: 23000000 INFO @ Sat, 15 Jan 2022 20:31:20: 15000000 INFO @ Sat, 15 Jan 2022 20:31:21: 19000000 INFO @ Sat, 15 Jan 2022 20:31:22: 24000000 INFO @ Sat, 15 Jan 2022 20:31:28: 16000000 INFO @ Sat, 15 Jan 2022 20:31:29: 20000000 INFO @ Sat, 15 Jan 2022 20:31:29: 25000000 INFO @ Sat, 15 Jan 2022 20:31:36: 17000000 INFO @ Sat, 15 Jan 2022 20:31:37: 26000000 INFO @ Sat, 15 Jan 2022 20:31:37: 21000000 INFO @ Sat, 15 Jan 2022 20:31:43: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:31:43: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:31:43: #1 total tags in treatment: 12375757 INFO @ Sat, 15 Jan 2022 20:31:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:31:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:31:43: #1 tags after filtering in treatment: 4783511 INFO @ Sat, 15 Jan 2022 20:31:43: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 15 Jan 2022 20:31:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:31:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:31:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:31:44: 18000000 INFO @ Sat, 15 Jan 2022 20:31:44: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 20:31:44: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:31:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:31:45: 22000000 INFO @ Sat, 15 Jan 2022 20:31:52: 19000000 INFO @ Sat, 15 Jan 2022 20:31:53: 23000000 INFO @ Sat, 15 Jan 2022 20:31:59: 20000000 INFO @ Sat, 15 Jan 2022 20:32:01: 24000000 INFO @ Sat, 15 Jan 2022 20:32:07: 21000000 INFO @ Sat, 15 Jan 2022 20:32:08: 25000000 INFO @ Sat, 15 Jan 2022 20:32:15: 22000000 INFO @ Sat, 15 Jan 2022 20:32:16: 26000000 INFO @ Sat, 15 Jan 2022 20:32:23: 23000000 INFO @ Sat, 15 Jan 2022 20:32:23: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:32:23: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:32:23: #1 total tags in treatment: 12375757 INFO @ Sat, 15 Jan 2022 20:32:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:23: #1 tags after filtering in treatment: 4783511 INFO @ Sat, 15 Jan 2022 20:32:23: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 15 Jan 2022 20:32:23: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:23: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 20:32:23: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:32:30: 24000000 INFO @ Sat, 15 Jan 2022 20:32:37: 25000000 INFO @ Sat, 15 Jan 2022 20:32:44: 26000000 INFO @ Sat, 15 Jan 2022 20:32:50: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:32:50: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:32:50: #1 total tags in treatment: 12375757 INFO @ Sat, 15 Jan 2022 20:32:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:50: #1 tags after filtering in treatment: 4783511 INFO @ Sat, 15 Jan 2022 20:32:50: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 15 Jan 2022 20:32:50: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:50: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 20:32:50: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781090/SRX11781090.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling