Job ID = 14520621 SRX = SRX11781087 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:27:32 prefetch.2.10.7: 1) Downloading 'SRR15481030'... 2022-01-15T10:27:32 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:29:03 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:29:03 prefetch.2.10.7: 1) 'SRR15481030' was downloaded successfully 2022-01-15T10:29:03 prefetch.2.10.7: 'SRR15481030' has 0 unresolved dependencies Read 17139146 spots for SRR15481030/SRR15481030.sra Written 17139146 spots for SRR15481030/SRR15481030.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:23 17139146 reads; of these: 17139146 (100.00%) were paired; of these: 2696922 (15.74%) aligned concordantly 0 times 12522459 (73.06%) aligned concordantly exactly 1 time 1919765 (11.20%) aligned concordantly >1 times ---- 2696922 pairs aligned concordantly 0 times; of these: 283848 (10.52%) aligned discordantly 1 time ---- 2413074 pairs aligned 0 times concordantly or discordantly; of these: 4826148 mates make up the pairs; of these: 2875280 (59.58%) aligned 0 times 1590302 (32.95%) aligned exactly 1 time 360566 (7.47%) aligned >1 times 91.61% overall alignment rate Time searching: 00:13:23 Overall time: 00:13:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2746298 / 14724208 = 0.1865 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:56:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:56:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:56:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:56:05: 1000000 INFO @ Sat, 15 Jan 2022 19:56:11: 2000000 INFO @ Sat, 15 Jan 2022 19:56:16: 3000000 INFO @ Sat, 15 Jan 2022 19:56:21: 4000000 INFO @ Sat, 15 Jan 2022 19:56:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:56:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:56:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:56:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:56:32: 6000000 INFO @ Sat, 15 Jan 2022 19:56:36: 1000000 INFO @ Sat, 15 Jan 2022 19:56:37: 7000000 INFO @ Sat, 15 Jan 2022 19:56:41: 2000000 INFO @ Sat, 15 Jan 2022 19:56:42: 8000000 INFO @ Sat, 15 Jan 2022 19:56:47: 3000000 INFO @ Sat, 15 Jan 2022 19:56:48: 9000000 INFO @ Sat, 15 Jan 2022 19:56:53: 4000000 INFO @ Sat, 15 Jan 2022 19:56:53: 10000000 BedGraph に変換中... INFO @ Sat, 15 Jan 2022 19:56:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:56:59: 11000000 INFO @ Sat, 15 Jan 2022 19:57:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:57:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:57:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:57:04: 6000000 INFO @ Sat, 15 Jan 2022 19:57:05: 12000000 INFO @ Sat, 15 Jan 2022 19:57:07: 1000000 INFO @ Sat, 15 Jan 2022 19:57:10: 7000000 INFO @ Sat, 15 Jan 2022 19:57:11: 13000000 INFO @ Sat, 15 Jan 2022 19:57:14: 2000000 INFO @ Sat, 15 Jan 2022 19:57:16: 8000000 INFO @ Sat, 15 Jan 2022 19:57:17: 14000000 INFO @ Sat, 15 Jan 2022 19:57:20: 3000000 INFO @ Sat, 15 Jan 2022 19:57:22: 9000000 INFO @ Sat, 15 Jan 2022 19:57:23: 15000000 INFO @ Sat, 15 Jan 2022 19:57:27: 4000000 INFO @ Sat, 15 Jan 2022 19:57:28: 10000000 INFO @ Sat, 15 Jan 2022 19:57:29: 16000000 INFO @ Sat, 15 Jan 2022 19:57:34: 5000000 INFO @ Sat, 15 Jan 2022 19:57:34: 11000000 INFO @ Sat, 15 Jan 2022 19:57:34: 17000000 INFO @ Sat, 15 Jan 2022 19:57:40: 18000000 INFO @ Sat, 15 Jan 2022 19:57:40: 12000000 INFO @ Sat, 15 Jan 2022 19:57:41: 6000000 INFO @ Sat, 15 Jan 2022 19:57:46: 19000000 INFO @ Sat, 15 Jan 2022 19:57:46: 13000000 INFO @ Sat, 15 Jan 2022 19:57:47: 7000000 INFO @ Sat, 15 Jan 2022 19:57:52: 20000000 INFO @ Sat, 15 Jan 2022 19:57:52: 14000000 INFO @ Sat, 15 Jan 2022 19:57:54: 8000000 INFO @ Sat, 15 Jan 2022 19:57:58: 21000000 INFO @ Sat, 15 Jan 2022 19:57:58: 15000000 INFO @ Sat, 15 Jan 2022 19:58:00: 9000000 INFO @ Sat, 15 Jan 2022 19:58:04: 22000000 INFO @ Sat, 15 Jan 2022 19:58:04: 16000000 INFO @ Sat, 15 Jan 2022 19:58:07: 10000000 INFO @ Sat, 15 Jan 2022 19:58:10: 23000000 INFO @ Sat, 15 Jan 2022 19:58:10: 17000000 INFO @ Sat, 15 Jan 2022 19:58:14: 11000000 INFO @ Sat, 15 Jan 2022 19:58:15: 24000000 INFO @ Sat, 15 Jan 2022 19:58:16: 18000000 INFO @ Sat, 15 Jan 2022 19:58:21: 12000000 INFO @ Sat, 15 Jan 2022 19:58:21: 25000000 INFO @ Sat, 15 Jan 2022 19:58:22: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:58:27: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:58:27: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:58:27: #1 total tags in treatment: 11723846 INFO @ Sat, 15 Jan 2022 19:58:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:58:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:58:27: #1 tags after filtering in treatment: 7062792 INFO @ Sat, 15 Jan 2022 19:58:27: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 19:58:27: #1 finished! INFO @ Sat, 15 Jan 2022 19:58:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:58:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:58:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:58:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:58:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:58:28: 13000000 INFO @ Sat, 15 Jan 2022 19:58:28: 20000000 INFO @ Sat, 15 Jan 2022 19:58:34: 21000000 INFO @ Sat, 15 Jan 2022 19:58:34: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:58:39: 22000000 INFO @ Sat, 15 Jan 2022 19:58:41: 15000000 INFO @ Sat, 15 Jan 2022 19:58:45: 23000000 INFO @ Sat, 15 Jan 2022 19:58:48: 16000000 INFO @ Sat, 15 Jan 2022 19:58:51: 24000000 INFO @ Sat, 15 Jan 2022 19:58:55: 17000000 INFO @ Sat, 15 Jan 2022 19:58:57: 25000000 INFO @ Sat, 15 Jan 2022 19:59:02: 18000000 INFO @ Sat, 15 Jan 2022 19:59:02: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:59:02: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:59:02: #1 total tags in treatment: 11723846 INFO @ Sat, 15 Jan 2022 19:59:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:59:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:59:02: #1 tags after filtering in treatment: 7062792 INFO @ Sat, 15 Jan 2022 19:59:02: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 19:59:02: #1 finished! INFO @ Sat, 15 Jan 2022 19:59:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:59:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:59:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:59:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:59:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:59:08: 19000000 INFO @ Sat, 15 Jan 2022 19:59:15: 20000000 INFO @ Sat, 15 Jan 2022 19:59:21: 21000000 INFO @ Sat, 15 Jan 2022 19:59:28: 22000000 INFO @ Sat, 15 Jan 2022 19:59:34: 23000000 INFO @ Sat, 15 Jan 2022 19:59:40: 24000000 INFO @ Sat, 15 Jan 2022 19:59:46: 25000000 INFO @ Sat, 15 Jan 2022 19:59:52: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:59:52: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:59:52: #1 total tags in treatment: 11723846 INFO @ Sat, 15 Jan 2022 19:59:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:59:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:59:52: #1 tags after filtering in treatment: 7062792 INFO @ Sat, 15 Jan 2022 19:59:52: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 19:59:52: #1 finished! INFO @ Sat, 15 Jan 2022 19:59:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:59:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:59:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:59:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:59:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781087/SRX11781087.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling