Job ID = 14520620 SRX = SRX11781086 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:27:32 prefetch.2.10.7: 1) Downloading 'SRR15481029'... 2022-01-15T10:27:32 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:29:14 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:29:14 prefetch.2.10.7: 1) 'SRR15481029' was downloaded successfully 2022-01-15T10:29:14 prefetch.2.10.7: 'SRR15481029' has 0 unresolved dependencies Read 21669088 spots for SRR15481029/SRR15481029.sra Written 21669088 spots for SRR15481029/SRR15481029.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:44 21669088 reads; of these: 21669088 (100.00%) were paired; of these: 2764148 (12.76%) aligned concordantly 0 times 13162189 (60.74%) aligned concordantly exactly 1 time 5742751 (26.50%) aligned concordantly >1 times ---- 2764148 pairs aligned concordantly 0 times; of these: 99670 (3.61%) aligned discordantly 1 time ---- 2664478 pairs aligned 0 times concordantly or discordantly; of these: 5328956 mates make up the pairs; of these: 3281278 (61.57%) aligned 0 times 1363559 (25.59%) aligned exactly 1 time 684119 (12.84%) aligned >1 times 92.43% overall alignment rate Time searching: 00:30:44 Overall time: 00:30:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2100803 / 19002630 = 0.1106 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:18:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:18:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:18:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:18:30: 1000000 INFO @ Sat, 15 Jan 2022 20:18:36: 2000000 INFO @ Sat, 15 Jan 2022 20:18:42: 3000000 INFO @ Sat, 15 Jan 2022 20:18:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:18:54: 5000000 INFO @ Sat, 15 Jan 2022 20:18:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:18:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:18:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:19:00: 6000000 INFO @ Sat, 15 Jan 2022 20:19:00: 1000000 INFO @ Sat, 15 Jan 2022 20:19:06: 2000000 INFO @ Sat, 15 Jan 2022 20:19:06: 7000000 INFO @ Sat, 15 Jan 2022 20:19:11: 3000000 INFO @ Sat, 15 Jan 2022 20:19:11: 8000000 INFO @ Sat, 15 Jan 2022 20:19:17: 4000000 INFO @ Sat, 15 Jan 2022 20:19:18: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:19:23: 5000000 INFO @ Sat, 15 Jan 2022 20:19:24: 10000000 INFO @ Sat, 15 Jan 2022 20:19:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:19:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:19:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:19:29: 6000000 INFO @ Sat, 15 Jan 2022 20:19:29: 11000000 INFO @ Sat, 15 Jan 2022 20:19:30: 1000000 INFO @ Sat, 15 Jan 2022 20:19:34: 7000000 INFO @ Sat, 15 Jan 2022 20:19:36: 12000000 INFO @ Sat, 15 Jan 2022 20:19:37: 2000000 INFO @ Sat, 15 Jan 2022 20:19:40: 8000000 INFO @ Sat, 15 Jan 2022 20:19:42: 13000000 INFO @ Sat, 15 Jan 2022 20:19:43: 3000000 INFO @ Sat, 15 Jan 2022 20:19:46: 9000000 INFO @ Sat, 15 Jan 2022 20:19:47: 14000000 INFO @ Sat, 15 Jan 2022 20:19:49: 4000000 INFO @ Sat, 15 Jan 2022 20:19:52: 10000000 INFO @ Sat, 15 Jan 2022 20:19:53: 15000000 INFO @ Sat, 15 Jan 2022 20:19:55: 5000000 INFO @ Sat, 15 Jan 2022 20:19:57: 11000000 INFO @ Sat, 15 Jan 2022 20:19:59: 16000000 INFO @ Sat, 15 Jan 2022 20:20:01: 6000000 INFO @ Sat, 15 Jan 2022 20:20:03: 12000000 INFO @ Sat, 15 Jan 2022 20:20:05: 17000000 INFO @ Sat, 15 Jan 2022 20:20:07: 7000000 INFO @ Sat, 15 Jan 2022 20:20:09: 13000000 INFO @ Sat, 15 Jan 2022 20:20:12: 18000000 INFO @ Sat, 15 Jan 2022 20:20:13: 8000000 INFO @ Sat, 15 Jan 2022 20:20:14: 14000000 INFO @ Sat, 15 Jan 2022 20:20:18: 19000000 INFO @ Sat, 15 Jan 2022 20:20:19: 9000000 INFO @ Sat, 15 Jan 2022 20:20:20: 15000000 INFO @ Sat, 15 Jan 2022 20:20:24: 20000000 INFO @ Sat, 15 Jan 2022 20:20:26: 10000000 INFO @ Sat, 15 Jan 2022 20:20:26: 16000000 INFO @ Sat, 15 Jan 2022 20:20:30: 21000000 INFO @ Sat, 15 Jan 2022 20:20:32: 11000000 INFO @ Sat, 15 Jan 2022 20:20:32: 17000000 INFO @ Sat, 15 Jan 2022 20:20:35: 22000000 INFO @ Sat, 15 Jan 2022 20:20:38: 12000000 INFO @ Sat, 15 Jan 2022 20:20:38: 18000000 INFO @ Sat, 15 Jan 2022 20:20:41: 23000000 INFO @ Sat, 15 Jan 2022 20:20:44: 13000000 INFO @ Sat, 15 Jan 2022 20:20:44: 19000000 INFO @ Sat, 15 Jan 2022 20:20:47: 24000000 INFO @ Sat, 15 Jan 2022 20:20:50: 14000000 INFO @ Sat, 15 Jan 2022 20:20:50: 20000000 INFO @ Sat, 15 Jan 2022 20:20:53: 25000000 INFO @ Sat, 15 Jan 2022 20:20:55: 15000000 INFO @ Sat, 15 Jan 2022 20:20:56: 21000000 INFO @ Sat, 15 Jan 2022 20:20:59: 26000000 INFO @ Sat, 15 Jan 2022 20:21:01: 16000000 INFO @ Sat, 15 Jan 2022 20:21:02: 22000000 INFO @ Sat, 15 Jan 2022 20:21:05: 27000000 INFO @ Sat, 15 Jan 2022 20:21:07: 17000000 INFO @ Sat, 15 Jan 2022 20:21:08: 23000000 INFO @ Sat, 15 Jan 2022 20:21:10: 28000000 INFO @ Sat, 15 Jan 2022 20:21:13: 18000000 INFO @ Sat, 15 Jan 2022 20:21:14: 24000000 INFO @ Sat, 15 Jan 2022 20:21:16: 29000000 INFO @ Sat, 15 Jan 2022 20:21:19: 19000000 INFO @ Sat, 15 Jan 2022 20:21:20: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:21:21: 30000000 INFO @ Sat, 15 Jan 2022 20:21:25: 20000000 INFO @ Sat, 15 Jan 2022 20:21:25: 26000000 INFO @ Sat, 15 Jan 2022 20:21:27: 31000000 INFO @ Sat, 15 Jan 2022 20:21:30: 21000000 INFO @ Sat, 15 Jan 2022 20:21:31: 27000000 INFO @ Sat, 15 Jan 2022 20:21:33: 32000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:21:36: 22000000 INFO @ Sat, 15 Jan 2022 20:21:37: 28000000 INFO @ Sat, 15 Jan 2022 20:21:38: 33000000 INFO @ Sat, 15 Jan 2022 20:21:41: 23000000 INFO @ Sat, 15 Jan 2022 20:21:43: 29000000 INFO @ Sat, 15 Jan 2022 20:21:44: 34000000 INFO @ Sat, 15 Jan 2022 20:21:47: 24000000 INFO @ Sat, 15 Jan 2022 20:21:48: 30000000 INFO @ Sat, 15 Jan 2022 20:21:50: 35000000 INFO @ Sat, 15 Jan 2022 20:21:53: 25000000 INFO @ Sat, 15 Jan 2022 20:21:54: 31000000 INFO @ Sat, 15 Jan 2022 20:21:54: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:21:54: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:21:54: #1 total tags in treatment: 16808321 INFO @ Sat, 15 Jan 2022 20:21:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:21:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:21:55: #1 tags after filtering in treatment: 8894597 INFO @ Sat, 15 Jan 2022 20:21:55: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 20:21:55: #1 finished! INFO @ Sat, 15 Jan 2022 20:21:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:21:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:21:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:21:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:21:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:21:58: 26000000 INFO @ Sat, 15 Jan 2022 20:21:59: 32000000 INFO @ Sat, 15 Jan 2022 20:22:04: 27000000 INFO @ Sat, 15 Jan 2022 20:22:05: 33000000 INFO @ Sat, 15 Jan 2022 20:22:10: 28000000 INFO @ Sat, 15 Jan 2022 20:22:11: 34000000 INFO @ Sat, 15 Jan 2022 20:22:16: 29000000 INFO @ Sat, 15 Jan 2022 20:22:17: 35000000 INFO @ Sat, 15 Jan 2022 20:22:21: 30000000 INFO @ Sat, 15 Jan 2022 20:22:22: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:22:22: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:22:22: #1 total tags in treatment: 16808321 INFO @ Sat, 15 Jan 2022 20:22:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:22:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:22:22: #1 tags after filtering in treatment: 8894597 INFO @ Sat, 15 Jan 2022 20:22:22: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 20:22:22: #1 finished! INFO @ Sat, 15 Jan 2022 20:22:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:22:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:22:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:22:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:22:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:22:27: 31000000 INFO @ Sat, 15 Jan 2022 20:22:32: 32000000 INFO @ Sat, 15 Jan 2022 20:22:38: 33000000 INFO @ Sat, 15 Jan 2022 20:22:44: 34000000 INFO @ Sat, 15 Jan 2022 20:22:50: 35000000 INFO @ Sat, 15 Jan 2022 20:22:55: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:22:55: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:22:55: #1 total tags in treatment: 16808321 INFO @ Sat, 15 Jan 2022 20:22:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:22:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:22:55: #1 tags after filtering in treatment: 8894597 INFO @ Sat, 15 Jan 2022 20:22:55: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 20:22:55: #1 finished! INFO @ Sat, 15 Jan 2022 20:22:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:22:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:22:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:22:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:22:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781086/SRX11781086.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling